1. Cell Cycle/DNA Damage Apoptosis
  2. CDK Apoptosis
  3. LDC000067

LDC000067  (Synonyms: LDC067)

Cat. No.: HY-15878 Purity: 98.00%
COA Handling Instructions

LDC000067 is a highly specific CDK9 inhibitor with an IC50 value of 44±10 nM in vitro.

For research use only. We do not sell to patients.

LDC000067 Chemical Structure

LDC000067 Chemical Structure

CAS No. : 1073485-20-7

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Solid + Solvent
10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 136 In-stock
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50 mg USD 504 In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products

    LDC000067 purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta. 2017 Nov 20;1865(2):354-363.  [Abstract]

    LAPC-4 cells are grown in charcoal-stripped serum medium (CSS) for 24 hours and then stimulated by R1881 alone or with different doses of LDC000067. LDC000067 is added 30 min before androgen treatment. Cellular expression of all proteins was detected by immunoblotting analysis. β-actin was included as a control for protein loading.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review


    LDC000067 is a highly specific CDK9 inhibitor with an IC50 value of 44±10 nM in vitro.

    IC50 & Target[1]

    CDK9- Cyclin T1

    44 nM (IC50)

    cdk2-cyclin A

    2441 nM (IC50)

    cdk1-cyclin B1

    5513 nM (IC50)

    cdk4-cyclin D1

    9242 nM (IC50)


    1460 nM (IC50)


    820 nM (IC50)


    3640 nM (IC50)

    In Vitro

    The selectivity of LDC000067 for CDK9 over other CDKs exceeds that of the known inhibitors flavopiridol and DRB. LDC000067 displayed 55/125/210/ >227/ >227-fold selectivity for CDK9 versus CDK2/1/4/6/7. LDC000067 inhibits in vitro transcription in an ATP-competitive and dose-dependent manner. Gene expression profiling of cells treated with LDC000067 demonstrates a selective reduction of short-lived mRNAs, including important regulators of proliferation and apoptosis[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.



    White to off-white




    Room temperature in continental US; may vary elsewhere.

    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 47 mg/mL (126.88 mM)

    *"≥" means soluble, but saturation unknown.

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6996 mL 13.4978 mL 26.9957 mL
    5 mM 0.5399 mL 2.6996 mL 5.3991 mL
    10 mM 0.2700 mL 1.3498 mL 2.6996 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.75 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.75 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (6.75 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation
    Kinase Assay

    The fluorescence resonance energy transfer (FRET)-based LANCE Ultra KinaSelect Ser/Thr kit is used to determine IC50 values for various CDK inhibitors. Briefly, a specific ULight MBP peptide substrate (50 nM final concentration) is phosphorylated by a CDK-cyclin pair in buffer (50 mM HEPES-KOH pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM dithiothreitol) containing ATP at the concentration of the Km values of the individual kinases for 1 h at room temperature. Subsequently, phosphorylation is detected by addition of specific Eu-labelled anti-phospho-antibodies (2 nM), which upon binding to the phosphopeptide give rise to a FRET signal. FRET signals are then recorded[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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    LDC000067 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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