Cat. No.: HY-N0212

Peimine

(Synonyms: Verticine; Dihydroisoimperialine)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Peimine (Verticine; Dihydroisoimperialine) is an orally active natural product. Peimine has anti-inflammatory, analgesic and cough relieving effects. Peimine can be used in cancer and inflammation related research.

For research use only. We do not sell to patients.

Purity: 99.9%
CAS No.: 23496-41-5
Formula: C₂₇H₄₅NO₃
Molecular Weight:431.65

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Peimine Chemical Structure

CAS No.: 23496-41-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Ki-67

PCNA

IL-1

IL-6

TNF-α

IL-1β

IL-10

p38

NFκB

All Interleukin Related Isoforms :

All TNF Receptor Isoforms :

All p38 MAPK Isoforms :

All NF-κB Isoforms :

In Vitro

Peimine (0-200 μM, 24 h) disrupts intracellular calcium homeostasis through the Ca²⁺/CaMKII/JNK pathway, inhibits the growth and movement of prostate cancer cells (DU-145, LNCap, and PC-3) and induces apoptosis, while having no significant effect on normal cells (RWPE-1) ^[3].
Peimine (0-25 mg/L, 1 h) has anti-inflammatory activity through regulating TNF-a, IL-6, IL-1b and IL-10 by suppressing the phosphorylation of MAPK and the activation of NF-kB in LPS-stimulated RAW 264.7 macrophages^[6].
Peimine (10-300 μM, 0-10 ms) can block the Nav1.7 ion channel with an IC₅₀ value of 47.2 μM, and can also inhibit the Kv1.3 ion channel with an IC₅₀ value of 354.4 μM^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

PC-3 cells
Concentration:

0-10 μM
Incubation Time:

24 h
Result:

Decreased the protein expression levels of Ki-67 and PCNA in PC-3 cells in a dose-dependent manner.

In Vivo

Peimine (32.5 μM/kg/day for 7days,i.g.) can effectively alleviate LPS-induced acute lung injury in mice, inhibit the expression levels of inflammatory factors TNF-α, IL-6, and IL-1 in mouse models, and has a synergistic effect when combined with Peiminine (HY-N0213) and Forsythoside. It can effectively improve its anti-inflammatory activity^[4].
The drug level of Peimine (single oral 4.25 g/kg) in the blood and tissue of male rats is significantly higher than that of female rats (Reference 3)^[5].

Pharmacokinetic Analysis in male and female SD Rats^[5]

Gender	Route	Dose (g/kg)	C_max (ng/mL)	T_max (h)	t_1/2 (h)	MRT_0-t (h)	MRT_0-∞ (h)	AUC_0-t (ng·h/mL)	AUC_0-∞ (ng·h/mL)	CL/F (L/kg/h)	V/F (L/kg)
Female	p.o.	4.25	43.7	1.5	4.2	7.8	7.9	214.2	214.3	128.9	781.3
Male	p.o.	4.25	57.6	2.9	6.2	7.2	7.4	662.4	665.3	41.5	374.1

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 23496-41-5
Appearance Solid
Molecular Weight 431.65
Formula C₂₇H₄₅NO₃
Color White to off-white
SMILES

[H][C@]12[C@]3([H])C[C@H](O)[C@@]4([H])C[C@@H](O)CC[C@]4(C)[C@@]3([H])C[C@]([H])1[C@]5([H])CN6C[C@@H](C)CC[C@]([H])6[C@@](C)(O)[C@]([H])5CC2
Synonyms

Verticine; Dihydroisoimperialine
Structure Classification
- Alkaloids
- Piperidine Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (4)

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Journal Impact Factor

Nat Commun

G-quadruplex as an essential structural element in cytomegalovirus replication origin. [Abstract]

2024 Aug 27;15(1):7353. PMID: 39191758
Phytomedicine

Total alkaloids of Fritillaria unibracteata var. wabuensis bulbus ameliorate chronic asthma via the TRPV1/Ca2+/NFAT pathway. [Abstract]

2023 Sep:118:154946. PMID: 37421766
Molecules

hERG Channel Blockade and Antagonistic Interactions of Three Steroidal Alkaloids from Fritillaria Species. [Abstract]

2025 Sep 25;30(19):3882. PMID: 41097303
Molecules

Metabolite Profiling and Anti-Inflammatory Activities of Fritillaria cirrhosa D. Don Bulbs Derived from Tissue Culture. [Abstract]

2025 Jan 31;30(3):623. PMID: 39942727

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (115.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3167 mL

11.5835 mL

23.1669 mL

5 mM

0.4633 mL

2.3167 mL

4.6334 mL

10 mM

0.2317 mL

1.1583 mL

2.3167 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yi PF, et al. Peimine impairs pro-inflammatory cytokine secretion through the inhibition of the activation of NF-κB and MAPK in LPS-induced RAW264.7 macrophages. Immunopharmacol Immunotoxicol. 2013 Oct;35(5):567-72. [Content Brief]

[2]. Chen K, Lv ZT, Zhou CH, et al. Peimine suppresses interleukin‑1β‑induced inflammation via MAPK downregulation in chondrocytes. Int J Mol Med. 2019;43(5):2241-2251. [Content Brief]

[3]. Tan H, et al. Peimine inhibits the growth and motility of prostate cancer cells and induces apoptosis by disruption of intracellular calcium homeostasis through Ca2+ /CaMKII/JNK pathway. J Cell Biochem. 2020 Jan;121(1):81-92. [Content Brief]

[4]. Liu C, et al. Synergistic anti-inflammatory effects of peimine, peiminine, and forsythoside a combination on LPS-induced acute lung injury by inhibition of the IL-17-NF-κB/MAPK pathway activation. J Ethnopharmacol. 2022 Sep 15;295:115343. [Content Brief]

[5]. Chen LH, et al. Sex dependent pharmacokinetics, tissue distribution and excretion of peimine and peiminine in rats assessed by liquid chromatography-tandem mass spectrometry. J Ethnopharmacol. 2013 Jan 9;145(1):77-84. [Content Brief]

[6]. Yi PF, et al. Peimine impairs pro-inflammatory cytokine secretion through the inhibition of the activation of NF-κB and MAPK in LPS-induced RAW264.7 macrophages. Immunopharmacol Immunotoxicol. 2013 Oct;35(5):567-72. [Content Brief]

[7]. Xu J, et al. Peimine, a main active ingredient of Fritillaria, exhibits anti-inflammatory and pain suppression properties at the cellular level. Fitoterapia. 2016 Jun;111:1-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3167 mL	11.5835 mL	23.1669 mL	57.9173 mL
	5 mM	0.4633 mL	2.3167 mL	4.6334 mL	11.5835 mL
	10 mM	0.2317 mL	1.1583 mL	2.3167 mL	5.7917 mL
	15 mM	0.1544 mL	0.7722 mL	1.5445 mL	3.8612 mL
	20 mM	0.1158 mL	0.5792 mL	1.1583 mL	2.8959 mL
	25 mM	0.0927 mL	0.4633 mL	0.9267 mL	2.3167 mL
	30 mM	0.0772 mL	0.3861 mL	0.7722 mL	1.9306 mL
	40 mM	0.0579 mL	0.2896 mL	0.5792 mL	1.4479 mL
	50 mM	0.0463 mL	0.2317 mL	0.4633 mL	1.1583 mL
	60 mM	0.0386 mL	0.1931 mL	0.3861 mL	0.9653 mL
	80 mM	0.0290 mL	0.1448 mL	0.2896 mL	0.7240 mL
	100 mM	0.0232 mL	0.1158 mL	0.2317 mL	0.5792 mL

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Peimine

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Publications Citing Use of MedChemExpress (MCE) Peimine

All Interleukin Related Isoforms

All TNF Receptor Isoforms

All p38 MAPK Isoforms

All NF-κB Isoforms

Biological Activity