AS 1411 sodium
Based on 3 publication(s) in Google Scholar
AS 1411 (AGRO-100) sodium is an oligonucleotide aptamer targeting nucleoproteins. AS 1411 sodium inhibits tumor cell proliferation by affecting the activity of nucleoprotein-containing complexes and can be used as a carrier to precisely deliver nanoparticles, oligonucleotides and small molecules to cancer cells. AS 1411 sodium reduces PRMT5 expression to inhibit tumor growth in DU145 prostate cancer cells. S 1411 sodium works by blocking the binding of nucleoproteins to bcl-2 mRNA in MCF-7 breast cancer cells. S 1411 sodium-coupled Jin nanospheres can inhibit breast cancer cell proliferation in vitro and in mouse models, has the ability to cross the blood-brain barrier with low tissue toxicity
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- 純度 : 91.58%
- 分子量:8272.31
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 AS 1411 sodium
MoreHistone Methyltransferase アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
IC50 & Target
[2]|
Bcl-2 |
体外実験
AS 1411 (10 μM, 0-48 h) sodium alters the nuclear localization of PRMT5 in DU145 cells[1].
AS 1411 (10 μM, 24 h) sodium reduces PRMT5 expression and increases the expression of cyclin E2 and ST7[1].
AS 1411 (0-20 μM, 0-96 h) sodium inhibits MCF-7 cell proliferation without affecting MCF-10A cells[2].
AS 1411 (2 μM, 1 h) sodium distributes in the nuclei of both MCF-7 and MCF-10A cells, but its cytoplasmic distribution is higher in MCF-7 cells[2].
AS 1411 (10 μM, 72 h) sodium inhibits the stability of Bcl-2 mRNA in MCF-7 and MDA-MB-231 cells, with no effect on MCF-10A cells[2].
AS 1411 (0-10 μM, 72 h) sodium inhibits the binding of nucleoproteins to bcl-2 mRNA in MCF-7 cells[2].
AS 1411 sodium conjugated with gold nanoparticles (0-800 nM, 72 h) exerts a stronger antiproliferative effect on MCF-7 and MDA-MB-231 cells compared to AS 1411 alone, without affecting MCF-10A cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7; MDA-MB-231; MCF-10A
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Concentration:0, 200, 400, 600, 800 nM
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Incubation Time:72 h
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Result:Inhibited MCF-7 cell survival by more than 80% at 200 nM, whereas neither AS1411 alone nor control GNS had a significant effect under these conditions. AS1411GNS (88% cell death at 200 nM) was significantly increased in MDA-MB-231 cells compared to AS1411 (27% cell death at 10 μM).
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Cell Line:MCF-7, MCF-10A
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Concentration:10 μM
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Incubation Time:72 h
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Result:Reduced the stability of bcl-2 mRNA, reducing the half-life of bcl-2 from 10h to 1h in MCF-7 cells and from 11h to 1.2h in MDA-MB-231 cells, but had no effect on MCF-10A cells.
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Cell Line:DU145; DU145 cells transfected with siRNA; NM23
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Concentration:10 μM
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Incubation Time:0, 1, 4, 24, 48 h
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Result:Reduced nucleolar protein-associated PRMT5 in the nucleus of DU145 cells and increased nucleolar protein-associated PRMT5 in the cytoplasm, which was related to time and dose.
Reduced the expression of PRMT5 in DU145 cells, upregulated the expression of PRMT5 cyclin2 and ST7 and inhibited tumor proliferation.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female nude mice bearing MDA-MB-231 xenografts[3]
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Dosage:22 μg; once a day; 12 days
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Administration:Intravenous injection (i.v.)
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Result:Took up and retained in tumors and cleared in normal tissues.
- RNA extraction experimental
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- Patient-Derived Orthotopic Xenograft (PDOX)
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Patient-Derived Xenograft (PDX)
- MTT Cell Proliferation Assay
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
化学情報
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性状 Solid
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分子量 8272.31
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Color White to off-white
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SMILES
[AS 1411 (sodium)]
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別名
AGRO-100 sodium
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Phytomedicine
2025 Aug:144:156932. PMID: 40482618 -
Int J Pharm X
An aptamer-conjugated mesoporous polydopamine formulation for synergistic targeted and photothermal therapy of hepatocellular carcinoma. [Abstract]2025 Apr 24:9:100335. PMID: 40927752 -
純度とドキュメンテーション
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データシート (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (1518 KB)
参考文献
[1]. Teng Y, et al. AS1411 alters the localization of a complex containing protein arginine methyltransferase 5 and nucleolin. Cancer Res. 2007;67(21):10491-10500. [Content Brief]
[2]. Soundararajan S, et al. The nucleolin targeting aptamer AS1411 destabilizes Bcl-2 messenger RNA in human breast cancer cells. Cancer Res. 2008;68(7):2358-2365. [Content Brief]
[3]. Mohammad T Malik, et al. AS1411-conjugated gold nanospheres and their potential for breast cancer therapy. Oncotarget. 2015 Sep 8;6(26):22270-81. [Content Brief]
Calculators
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AS 1411 sodium 関連分類
HY-147081A 関連分類
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