Art. -Nr.: HY-N0322

Cholesterol (from animal)

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Based on 92 publication(s) in Google Scholar

Cholesterol (from animal) is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol (from animal) plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins. Cholesterol (from animal) is also an endogenous estrogen-related receptor α (ERRα) agonist.

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Reinheit: 99.94%
CAS. Nr.: 57-88-5
Formel: C₂₇H₄₆O
Molecular Weight:386.65

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Cholesterol (from animal) Chemical Structure

CAS. Nr.: 57-88-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (92)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cholesterol (from animal) is the major sterol in mammals. It is making up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol (from animal) plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins^[1]^[2]. Cholesterol (from animal) is also an endogenous estrogen-related receptor α (ERRα) agonist^[3].

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Alle Estrogen Receptor/ERR Isoform-spezifische Produkte anzeigen :

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>20 μM Compound: b1	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 30822712]
BJ	IC₅₀	>50 μM Compound: Cholesterol	Cytotoxicity against human BJ cells after 72 hrs by calcein AM assay Cytotoxicity against human BJ cells after 72 hrs by calcein AM assay	[PMID: 22417637]
CCRF-CEM	IC₅₀	>50 μM Compound: Cholesterol	Cytotoxicity against human CEM cells after 72 hrs by calcein AM assay Cytotoxicity against human CEM cells after 72 hrs by calcein AM assay	[PMID: 22417637]
HeLa	IC₅₀	>50 μM Compound: Cholesterol	Cytotoxicity against human HeLa cells after 72 hrs by calcein AM assay Cytotoxicity against human HeLa cells after 72 hrs by calcein AM assay	[PMID: 22417637]
HL-60	IC₅₀	>20 μM Compound: b1	Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 30822712]
HT-29	IC₅₀	>50 μM Compound: 1	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
MCF7	IC₅₀	>20 μM Compound: b1	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 30822712]
MCF7	IC₅₀	>50 μM Compound: Cholesterol	Cytotoxicity against human MCF7 cells after 72 hrs by calcein AM assay Cytotoxicity against human MCF7 cells after 72 hrs by calcein AM assay	[PMID: 22417637]
SMMC-7721	IC₅₀	>20 μM Compound: b1	Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 30822712]
SW480	IC₅₀	>20 μM Compound: b1	Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay	[PMID: 30822712]
Vero	IC₅₀	>128 μg/mL Compound: 6	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 18818073]

In Vitro

GT1-7 hypothalamic cells subjected to Cholesterol depletion in vitro produced 20-31% reductions in cellular Cholesterol content. All Cholesterol-depleted neuron-derived cells, exhibit decreased phosphorylation/activation of IRS-1 and AKT following stimulation by insulin, insulin-like growth factor-1, or the neurotrophins (NGF and BDNF). Reduction in cellular Cholesterol also results in increased basal autophagy and impairment of induction of autophagy by glucose deprivation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cholesterol can be used to create models of hyperlipidemia and atherosclerosis. The metabolic half-life of Cholesterol varies from a few hours to several years, depending on its association with different lipoproteins and the specific tissues in which it is located^[4].

Induction of Hyperlipidemia^[5]^[6]

Background

Hyperlipidemia is a group of disorders characterized by elevated concentrations of circulating lipids, including cholesterol, cholesterol esters, phospholipids and triglycerides. If the intake of cholesterol is too much, and exceeds the body's metabolic capacity, it may lead to increased plasma cholesterol levels, causing hyperlipidemia.

Specific Modeling Methods

Rat: Wistar • male • 18-week-old (period: 8 weeks)
Administration: 2% cholesterol; diet • 8 weeks

Note

(1) Rats were housed in a room maintained at a 12-h light-dark cycle and a constant temperature of 22±2 °C
(2) Wistar rats were always chosen for hyperlipidemia studies since this species shows a moderate increase in serum cholesterol and triglyceride level due to a high-cholesterol diet and no substantial atherosclerosis develops; therefore, the direct myocardial effect of hyperlipidemia, independent from atherosclerosis, can be studied in this model.

Modeling Indicators

Molecular changes: Significant increase in total cholesterol levels in blood samples (about 20%)

Correlated Product(s): /

Opposite Product(s): /

Induction of Atherosclerosis^[7]^[8]

Background

High levels of cholesterol in the blood, especially low-density lipoprotein cholesterol (LDL-C), can accumulate plaque on the walls of blood vessels, a process known as atherosclerosis. Over time, these plaques can block blood flow and cause serious health problems such as myocardial ischemia or myocardial infarction.

Specific Modeling Methods

Rabbits: Oryctolagus cuniculus • male • 4-6-month-old (period: 16 weeks)
Administration: 0.3% cholesterol and 3% soybean oil; diet • 16 weeks

Note

(1) The cholesterol-fed rabbit is a widely used model for experimental atherosclerosis research as cholesterol only cause atherosclerotic changes in the rabbit arterial intima, which was very similar to human atherosclerosis.
(2) As the absorption of dietary cholesterol requires fat, you must add oil into the diet. Otherwise, rabbits will use their internal fat, which makes them lean or sick. In addition, using soybean oil, which consists of unsaturated fatty acids, can prevent the levels of plasma cholesterol from becoming too high. Other vegetable oils, such as peanut oil or corn oil, can be used because they are all unsaturated fatty acids. Animal fat (saturated fatty acids) like tallow and lard is not recommended.
(3) 0.3-0.5% cholesterol diet is recommended for most experiments. Rabbits cannot tolerate a 1-2% cholesterol diet for a month as they develop severe liver dysfunction.
(4) Adult rabbits at 4 months or older can consume approximately ~150 g a day. You can either feed ab libitum or restricted (100-150 g/day/adult rabbit).
(5) Plasma lipids should be measured weekly, especially for the first 4 weeks, because you need to determine whether plasma levels of cholesterol are elevated in each animal. Non-responder rabbits can be excluded from the experiments if their plasma cholesterol levels do not increase after cholesterol diet feeding.
(6) Plasma lipoproteins can be measured at 8 and 16 weeks when the plasma levels of cholesterol are stable.
(7) The age of rabbits should be considered because young rabbits are more susceptible to aortic atherosclerosis than old rabbits even though they have similar plasma cholesterol levels. 4-6-month-old rabbits are usually used for cholesterol feeding experiments.
(8) Male and female rabbits are different in terms of response to a cholesterol diet and atherosclerosis. In our experience, female rabbits develop higher hypercholesterolemia and greater aortic lesions than their counterpart male rabbits. In general, male rabbits are recommended for experiments because estrogen may influence the results.

Modeling Indicators

Histological changes: atherosclerosis lesions can be seen on HE stained aortic arch and thoracic aorta segments

Correlated Product(s): Soybean oil (HY-108750)

Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 57-88-5
Appearance Solid
Molecular Weight 386.65
Formel C₂₇H₄₆O
Color White to off-white
SMILES

O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@@H](CCCC(C)C)C
Structure Classification
- Steroids
Initial Source
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (92)

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Journal Impact Factor

Signal Transduct Target Ther

Intranasal prime-boost RNA vaccination elicits potent T cell response for lung cancer therapy. [Abstract]

2025 Mar 24;10(1):101. PMID: 40122855
Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Nat Nanotechnol

Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase. [Abstract]

2021 Oct;16(10):1150-1160. PMID: 34354264
Mol Cancer

CircABCA1 promotes ccRCC by reprogramming cholesterol metabolism and facilitating M2 macrophage polarization through IGF2BP3-mediated stabilization of SCARB1 mRNA. [Abstract]

2025 Jul 19;24(1):199. PMID: 40684174
Mol Cancer

GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis. [Abstract]

2025 May 26;24(1):151. PMID: 40414849

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]

Analysis of interactions between GP73 and Cholesterol (0.1-10000 nM, 3 h), 25-HC, 27-HC, campesterol, and epicholesterol by MST.

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]

Analysis of interactions between Cholesterol (0.1-1000 nM, 3 h) and mouse GP73 (WT), the GP73 CRAC domain deletion mutant (∆CRAC) and the CARC Phe90/CRAC Tyr99 double mutant of mouse GP73 (F90I-Y99I, DM) by MST.

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2025 May 26;24(1):151. [Abstract]

Titration curves showing changes in the intrinsic fluorescence of WT, Y99I, F90I-Y99I (DM), or scrambled peptides (5 µM) in the presence of increasing concentrations of Cholesterol (0-140 μM).
Cell Metab

Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury. [Abstract]

2024 Nov 5;36(11):2402-2418.e10. PMID: 39389061
Nat Biomed Eng

Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells. [Abstract]

2025 Feb;9(2):215-233. PMID: 39730914
Immunity

Modulation of the mevalonate pathway by TCR engagement regulates T follicular helper cell generation in homeostasis and autoimmunity. [Abstract]

2026 May 13:S1074-7613(26)00171-8. PMID: 42134327
Immunity

25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages. [Abstract]

2024 May 14;57(5):1087-1104.e7. PMID: 38640930
Cancer Commun (Lond)

Lipid metabolism reprograming by SREBP1-PCSK9 targeting sensitizes pancreatic cancer to immunochemotherapy. [Abstract]

2025 Aug;45(8):1010-1037. PMID: 40439109
Nat Aging

Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPases. [Abstract]

2025 Aug 26. PMID: 40858817
Cell Host Microbe

A CLOCK-targeting lncRNA induces trained immunity against tuberculosis. [Abstract]

2025 Dec 30:S1931-3128(25)00521-9. PMID: 41475336
Nat Commun

Tubeimosides are pan-coronavirus and filovirus inhibitors that can block their fusion protein binding to Niemann-Pick C1. [Abstract]

2024 Jan 2;15(1):162. PMID: 38167417

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jan 2;15(1):162. [Abstract]

Detection of the exogenous cholesterol activity in SARS2 infection. Indicated Caco2 cells were infected with SARS2-Spseudoviruses and treated with cholesterol (0-200 μg/mL) at indicated concentrations. After 48 hours, viral infection was determined by measuring the intracellular luciferase activity. RLU, relative light unit.
Adv Sci (Weinh)

Targeting the HSPA8-CMA-ATP6V1A Axis Triggers Lysosomal Hyperacidification and Catastrophic Vacuolation in Prostate Cancer. [Abstract]

2026 Jun 19:e76165. PMID: 42318659
Adv Sci (Weinh)

An Insulin-Exosome-TNFAIP8 Axis Drives Stromal Fibrosis and Therapeutic Resistance in Pancreatic Cancer. [Abstract]

2026 Apr;13(24):e15606. PMID: 41709860
Adv Sci (Weinh)

Brachyury-Activated Fucoidan Hydrogel Microspheres Rejuvenate Degenerative Intervertebral Discs Microenvironment. [Abstract]

2025 Jun 20:e04195. PMID: 40539715

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun 20:e04195. [Abstract]

Dil staining of GF and BLNP （the formulation (50:38.5:10:1.5) included ionizable lipids (D‐Lin‐MC3‐DMA), Cholesterol, DSPC, and DMG‐PEG2000‐NH2, which were homogeneously mixed in the ethanol phase (12 mM)）@GF microspheres (scale bar = 50 µm).
Adv Sci (Weinh)

CircUGP2 Suppresses Intrahepatic Cholangiocarcinoma Progression via p53 Signaling Through Interacting With PURB to Regulate ADGRB1 Transcription and Sponging miR-3191-5p. [Abstract]

2024 Aug 9:e2402329. PMID: 39120980
Adv Sci (Weinh)

Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle. [Abstract]

2024 Jul 1:e2403640. PMID: 38946588
Adv Sci (Weinh)

Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC. [Abstract]

2023 Sep;10(27):e2206878. PMID: 37490552

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]

Effects of cholesterol repletion (20 μg/mL; 4 days) on colony formation potential in SCC-1cisR and SCC-23cisR cells with SQLE depletion in the presence of cisplatin (n = 4) were assessed.

Cholesterol (from animal) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Sep;10(27):e2206878. [Abstract]

SCC-1cisR and SCC-23cisR cells transfected with siCTRL or siSQLE were incubated in medium with or without 20 μg/mL Cholesterol for 24 h.
Nat Chem Biol

Structures of the ADGRG2-Gs complex in apo and ligand-bound forms. [Abstract]

2022 Nov;18(11):1196-1203. PMID: 35982227
Theranostics

ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3. [Abstract]

2021 Jan 1;11(2):841-860. PMID: 33391508
Exp Mol Med

Cholesterol biosynthesis induced by radiotherapy inhibits cGAS-STING activation and contributes to colorectal cancer treatment resistance. [Abstract]

2025 May;57(5):1089-1105. PMID: 40355720
J Nanobiotechnology

Targeted delivery and ROS-responsive release of Resolvin D1 by platelet chimeric liposome ameliorates myocardial ischemia-reperfusion injury. [Abstract]

2022 Oct 20;20(1):454. PMID: 36266658
Redox Biol

Dysregulated lipids homeostasis disrupts CHAC1-mediated ferroptosis driving fibroblast growth factor receptor tyrosine kinase inhibitor AZD4547 resistance in gastric cancer. [Abstract]

2025 Jul:84:103693. PMID: 40460553
Redox Biol

Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironment. [Abstract]

2023 Jun:62:102678. PMID: 36940607
J Control Release

Antimicrobial peptide-derived lipid nanoparticles enable lung-targeted mRNA therapy for multidrug-resistant bacterial pneumonia. [Abstract]

2026 May 23:396:115047. PMID: 42178062
J Control Release

Impact of ionizable lipid source on quality and stability of siRNA-loaded lipid nanoparticles. [Abstract]

2026 Mar 14:114825. PMID: 41839265
J Control Release

Functional lipid nanoparticles for safe delivery of macromolecular antibiotics to gram-negative bacteria. [Abstract]

2025 May 30:113907. PMID: 40451556
Cell Rep Med

Synthetic cleavage-resistant TREM2 boosts macrophage efferocytosis to treat inflammatory diseases. [Abstract]

2026 Feb 17;7(2):102580. PMID: 41616764
Cell Rep Med

Synergistic mechanical and therapeutic modulation of engineered tumor cell-derived microparticles for enhanced cancer treatment. [Abstract]

2026 Feb 17;7(2):102591. PMID: 41666924
Mater Today Bio

Ultrasound-triggered dissolving microneedle platform for cGAS-STING-mediated precision immunotherapy of melanoma. [Abstract]

2026 Mar 2:37:102990. PMID: 41815866
Nat Struct Mol Biol

Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS. [Abstract]

2025 May;32(5):896-904. PMID: 39824976
Adv Healthc Mater

Liposomal Quercetin-Loaded Biomimetic Scaffolds Manage Mechanical Overload in Maxillofacial Regeneration by Stabilizing the β-Actin/YAP Mechanosensing Axis. [Abstract]

2026 Jun;15(22):e05818. PMID: 42027072
Adv Healthc Mater

An Injectable Liposome-Hydrogel Composite for Targeted Delivery of a Non-Lactylated Peptide to Rebalance Bone Metabolism in Hyperlipidemia. [Abstract]

2026 Feb 21:e05684. PMID: 41721628
Sci China Life Sci

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry. [Abstract]

2022 Feb;65(2):341-361. PMID: 34047913
Small Methods

Cholesterol-Derived Mannosylated Polypeptide-Formed Lipid Nanoparticles for Efficient in Vivo mRNA Delivery. [Abstract]

2025 Apr 21:e2401712. PMID: 40256901
Proc Natl Acad Sci U S A

2026 Jul 7;123(27):e2523789123. PMID: 42384696
Phytomedicine

Disruption of cholesterol homeostasis triggers NLRP3-cGAS-STING axis-dependent hepatic fibrosis and honokiol intervention effects. [Abstract]

2025 May 24:143:156904. PMID: 40449452
EMBO Mol Med

LILRB1-HLA-G axis defines a checkpoint driving natural killer cell exhaustion in tuberculosis. [Abstract]

2024 Aug;16(8):1755-1790. PMID: 39030302
Phytomedicine

Diosgenin as a substitute for cholesterol alleviates NAFLD by affecting CYP7A1 and NPC1L1-related pathway. [Abstract]

2024 Mar:125:155299. PMID: 38301301
ACS Appl Mater Interfaces

Feedback-Amplified Drug Delivery of αPD-L1-Modified Lipoplatin for Chronological Synergy in NSCLC through PD-L1 Upregulation. [Abstract]

2025 Oct 8;17(40):55861-55875. PMID: 40997865
Free Radic Biol Med

25-Hydroxycholesterol inhibits Hantavirus infection by reprogramming cholesterol metabolism. [Abstract]

2024 Aug 27:S0891-5849(24)00623-3. PMID: 39209137
ACS Appl Mater Interfaces

Size-Controllable and Monodispersed Lipid Nanoparticle Production with High mRNA Delivery Efficiency Using 3D-Printed Ring Micromixers. [Abstract]

2024 Sep 4;16(35):46044-46052. PMID: 39103250
Cell Death Discov

Inhibition of lanosterol synthase linking with MAPK/JNK signaling pathway suppresses endometrial cancer. [Abstract]

2025 Feb 8;11(1):55. PMID: 39922821
Cell Death Discov

Upregulation of rate-limiting enzymes in cholesterol metabolism by PKCδ mediates endothelial apoptosis in diabetic wound healing. [Abstract]

2024 May 29;10(1):263. PMID: 38811564
Int J Nanomedicine

Intraperitoneal Co-Delivery of Claudin18.2×41BB and EpCAM×CD3 Bispecific Antibodies via mRNA-LNPs Synergistically Suppresses Gastric Cancer Peritoneal Metastasis Through T Cell Co-Activation. [Abstract]

2026 Mar 20:21:577606. PMID: 41884275
Int J Nanomedicine

Optimized Lipid Nanoparticle-Mediated mRNA Co-Delivery of SOX5/SOX9 Enables Synergistic Senescence Reversal for Osteoarthritis Therapy. [Abstract]

2025 Oct 22:20:12783-12804. PMID: 41146650
NPJ Vaccines

A self-amplifying mRNA vaccine expressing PRV gD induces robust immunity against virulent mutants. [Abstract]

2025 Aug 14;10(1):193. PMID: 40813770
Cell Biosci

Cholesterol confers resistance to Apatinib-mediated ferroptosis in gastric cancer. [Abstract]

2025 Jul 4;15(1):95. PMID: 40615893
Cell Mol Life Sci

Human cytomegalovirus infection impairs neural differentiation via repressing sterol regulatory element binding protein 2-mediated cholesterol biosynthesis. [Abstract]

2024 Jul 6;81(1):289. PMID: 38970696
Anal Chim Acta

2026 Jun 16;1418:345855.
Polym Test

2023 Dec 1, 108292.
Plant J

Unveiling the inequivalent biochemical functions of OsSMO2-1 and OsSMO2-2 in rice phytosterol biosynthesis using a customized sterol standards mixture. [Abstract]

2025 Nov;124(3):e70559. PMID: 41194406
Biochem Pharmacol

Triptolide induces the secretion of insulin, through which it alleviates the tauopathies of Alzheimer's disease via inhibiting the phosphorylation of tau. [Abstract]

2025 Aug 8;242(Pt 3):117223. PMID: 40784568
Biomacromolecules

Replacing PEG-Lipid with Amphiphilic Polycarbonates in mRNA-Loaded Lipid Nanoparticles: Impact of Polycarbonate Structure on Physicochemical and Transfection Properties. [Abstract]

2025 Jun 9;26(6):3563-3575. PMID: 40347133
J Ethnopharmacol

Tylophora yunnanensis extract inhibits cholesterol biosynthesis to suppress triple negative breast cancer. [Abstract]

2025 Apr 24:119877. PMID: 40287115
Int J Pharm

Development of a proliposomal pretomanid dry powder inhaler as a novel alternative approach for combating pulmonary tuberculosis. [Abstract]

2024 Aug 18:124608. PMID: 39163929
Commun Biol

SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2. [Abstract]

2026 Feb 7;9(1):373. PMID: 41654690
Life Sci

CKN reduces TLR4-mediated inflammation and cerebral I/R injury by activating the LXRα/ABCA1 pathway in microglia. [Abstract]

2025 Jun 1:370:123571. PMID: 40107493
Commun Biol

Mevalonate pathway inhibition reduces bladder cancer metastasis by modulating RhoB protein stability and integrin β1 localization. [Abstract]

2024 Nov 9;7(1):1476. PMID: 39521858
Life Sci

Cordycepin reprogramming lipid metabolism to block metastasis and EMT via ERO1A/mTOR/SREBP1 axis in cholangiocarcinoma. [Abstract]

2023 Aug 15:327:121698. PMID: 37080351
One Health Adv

2026 Jun 11;4(1):17.
Respir Res

Activation of LXR signaling ameliorates apoptosis of alveolar epithelial cells in Bronchopulmonary dysplasia. [Abstract]

2024 Nov 7;25(1):399. PMID: 39511537
Bioorg Chem

Design and synthesis of novel ionizable lipids enables highly efficient mRNA delivery via lipid nanoparticles. [Abstract]

2026 Apr:171:109526. PMID: 41621179
Nanomedicine

Quantitative analysis of mRNA-lipid nanoparticle stability in human plasma and serum by size-exclusion chromatography coupled with dual-angle light scattering. [Abstract]

2024 Jun:58:102745. PMID: 38499167
iScience

CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer. [Abstract]

2024 Apr 6;27(5):109676. PMID: 38665208
Antiviral Res

Targeting 7-dehydrocholesterol reductase against EV-A71 replication by upregulating interferon response. [Abstract]

2023 Jan:209:105497. PMID: 36528172
Sci Rep

Serum starvation-induced cholesterol reduction increases melanoma cell susceptibility to cytotoxic T lymphocyte killing. [Abstract]

2025 May 26;15(1):18364. PMID: 40419522
J Pharmacol Exp Ther

2025 Dec 24.
J Pharm Sci

Development of new nanoniosome carriers for vorinostat: Evaluation of anticancer efficacy in vitro. [Abstract]

2024 May 25:S0022-3549(24)00199-0. PMID: 38801974
Insect Biochem Mol Biol

Interaction of ML1 and SRB1-12 mediates cholesterol transport in the brown planthopper. [Abstract]

2026 Mar:188:104499. PMID: 41548698
Cell Signal

Activated PARP1/FAK/COL5A1 signaling facilitates the tumorigenesis of cholesterol-resistant ovarian cancer cells through promoting EMT. [Abstract]

2024 Sep 16:111419. PMID: 39293744
Heliyon

2026 Jan 22;12(2):e44534.
Viruses

Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection. [Abstract]

2022 Mar 3;14(3):514. PMID: 35336921
Pathol Res Pract

Alendronate-functionalized ZIF-8 nanoplatform for targeted delivery of SHH siRNA and docetaxel to treat prostate cancer bone metastasis. [Abstract]

2025 Sep:273:156138. PMID: 40694986
Part Part Syst Charact

2024 Jun 12.
Life Sci Alliance

Necrotic debris and STING exert therapeutically relevant effects on tumor cholesterol homeostasis. [Abstract]

2022 Jan 4;5(3):e202101256. PMID: 34983824
Vet Microbiol

Pseudorabies virus EP0 recruits TECPR2 and CK2 to promote COPII accumulation and viral egress. [Abstract]

2026 Jun 17:320:111115. PMID: 42322707
Vet Microbiol

The activation of liver X receptors in Madin-Darby bovine kidney cells and mice restricts infection by bovine viral diarrhea virus. [Abstract]

2024 Jan:288:109948. PMID: 38113573
Biochem Biophys Res Commun

Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]

2020 Feb 19;522(4):862-868. PMID: 31806372
Lett Drug Des Discov

2026 Feb 11.
Part Sci Technol

2025 Jul 03.
Gene Rep

2026 Jan 12;42:102429.
SSRN

2025 Oct 29.
Res Sq

2025 Jul 29.
Cell Biomater

2025 Aug 19.
SSRN

2025 Aug 29.
Patent

US20250248939A1.
Traditional Medicine Research

2025;10(4):20.
SSRN

2024 Feb 5.
Patent

US20200376146A1.
Patent

US20200376102A1.

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 10 mg/mL (25.86 mM; ultrasonic and warming and heat to 60°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5863 mL

12.9316 mL

25.8632 mL

5 mM

0.5173 mL

2.5863 mL

5.1726 mL

10 mM

0.2586 mL

1.2932 mL

2.5863 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.43 mg/mL (3.70 mM); Clear solution

This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 1.43 mg/mL (3.70 mM); Clear solution

This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (14.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (396 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
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Handling Instructions (2659 KB)

Verweise

[1]. Casaburi I, et al. Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment. Front Endocrinol (Lausanne). 2018 Sep 11;9:525. [Content Brief]

[2]. Dietschy JM, et al. Thematic review series: brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal. J Lipid Res. 2004 Aug;45(8):1375-97. [Content Brief]

[3]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14. [Content Brief]

[4]. Puskás LG, et al. Cholesterol diet-induced hyperlipidemia influences gene expression pattern of rat hearts: a DNA microarray study. FEBS Lett. 2004 Mar 26;562(1-3):99-104. [Content Brief]

[5]. Onody A, et al. Hyperlipidemia induced by a cholesterol-rich diet leads to enhanced peroxynitrite formation in rat hearts. Cardiovasc Res. 2003 Jun 1;58(3):663-70. [Content Brief]

[6]. Baumer Y, et al. Hyperlipidemia-induced cholesterol crystal production by endothelial cells promotes atherogenesis. Nat Commun. 2017 Oct 24;8(1):1129. [Content Brief]

[7]. Finking G, et al. Nikolaj Nikolajewitsch Anitschkow (1885-1964) established the cholesterol-fed rabbit as a model for atherosclerosis research. Atherosclerosis. 1997 Nov;135(1):1-7. [Content Brief]

[8]. Fan J, et al. Use of Rabbit Models to Study Atherosclerosis. Methods Mol Biol. 2022;2419:413-431. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	2.5863 mL	12.9316 mL	25.8632 mL	64.6580 mL
	5 mM	0.5173 mL	2.5863 mL	5.1726 mL	12.9316 mL
	10 mM	0.2586 mL	1.2932 mL	2.5863 mL	6.4658 mL
	15 mM	0.1724 mL	0.8621 mL	1.7242 mL	4.3105 mL
	20 mM	0.1293 mL	0.6466 mL	1.2932 mL	3.2329 mL
	25 mM	0.1035 mL	0.5173 mL	1.0345 mL	2.5863 mL

Cholesterol (from animal)

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Verweise

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Complete Stock Solution Preparation Table

Cholesterol (from animal) Related Classifications

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