Cat. No.: HY-15311

Avermectin B1

(Synonyms: Abamectin; Avermectin B1a-Avermectin B1b mixt.)

5 Cited Publications

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Based on 5 publication(s) in Google Scholar

Avermectin B1 (Abamectin) is a mixture of two similar segments of avermectin. Avermectin B1 is an orally anti-infection agent, which can be used in the research of parasitic worms, insect pests, agriculture and animal husbandry. Avermectin B1 can also induce the production of ROS and induces cytotoxicity, apoptosis and autophagy.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Pureté: 99.48%
CAS No.: 71751-41-2
Formule: C₄₈H₇₂O₁₄ (for Avermectin B1a)
Masse moléculaire:873.09

Stockage:

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Avermectin B1 Chemical Structure

CAS No.: 71751-41-2

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Activité biologique
- Description
- In Vitro
- In Vivo
- Essai clinique
Chemical Information
Publications (5)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

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In Vitro

Avermectin B1 (0-80 μM, 12 h) induces cytotoxicity through MAPK and ATM/ATR pathway in mouse embryonic fibroblast (MEF) cells, and induces ROS-mediated DNA damage^[1].
Avermectin B1 (36 μg/mL, 72 h) has strong nematicidal efect of on G. pallida in aqueous solution, and negatively influences viability and infectivity of G. pallida J2 detected in potato roots(cv. Spunta)^[2].
Abamectin (10 μM, 24 h) induces significant cytotoxicity by overproduction of ROS in haemocytes of Erocheir sinensis^[3].
Abamectin (4 μM, 24 h) induces apoptosis and autophagy by inhibiting ROS-mediated PI3K/AKT signaling in MGC803 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Mouse embryonic fibroblast (MEF) cells
Concentration:

0, 0.5, 5, 10, 20, 40, 80 μM
Incubation Time:

12 h
Result:

Reduced cell viability with an IC₅₀ value of 45.6 μM.

Western Blot Analysis^[3]

Cell Line:

MGC803 cells
Concentration:

0-4 μM
Incubation Time:

24 h
Result:

Increased active caspase-3 and expression of Bax/Bcl-2, decreased MMP in a dose-dependent manner.

In Vivo

Avermectin B1 (oral administration, 0.2 mg/kg for a single dose) is highly efficacious against intestinal strongyles and Onchocera microfilaria in horses^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Horses (had a pruritic dermatosis)^[5]
Dosage:

0.2 mg/kg, a single dose.
Administration:

Oral administration
Result:

Decreased in mean strongyle egg counts 14, 28 and 42 d after treatment, and resulted in zero microfilaria counts in all horses 14 d after treatment.

Animal Model:

Healthy adult female sheep (Pharmacokinetic assay)^[6]
Dosage:

0.2 mg/kg
Administration:

Subcutaneous administration (in the left neck area of each sheep)

Result:

Pharmacokinetic profiles of Avermectin B1

Parameters	Mean
K_cl (/day)	0.17
t_1/2cl (day)	4.36
K_ab (/day)	0.24
t_1/2ab (day)	3.15
C_max (ng/mL)	6.24
t_max (day)	4.20
AUC_(0-27) (ng/day/mL)	80.2
AU_C(0-∞) (ng/day/mL)	84.7
MRT (day)	8.80

Essai clinique

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 71751-41-2
Appearance Solid
Masse moléculaire 873.09
Formule C₄₈H₇₂O₁₄ (for Avermectin B1a)
Color White to off-white
SMILES

C[C@@H](CC)[C@]1([H])[C@@H](C)C=C[C@@]2(O[C@@]3([H])C[C@]([H])(OC([C@@]4([H])[C@@](/C(CO5)=C/C=C/[C@H](C)[C@H](O[C@@]6([H])C[C@H](OC)[C@@H](O[C@]7([H])O[C@@H](C)[C@H](O)[C@@H](OC)C7)[C@H](C)O6)/C(C)=C/C3)(O)[C@@]5([H])[C@H](O)C(C)=C4)=O)C2)O1.C[C@H]8C=C[C@@]9(O[C@@]%10([H])C[C@]([H])(OC([C@@]%11([H])[C@@](/C(CO%12)=C/C=C/[C@H](C)[C@H](O[C@@]%13([H])C[C@H](OC)[C@@H](O[C@]%14([H])O[C@@H](C)[C@H](O)[C@@H](OC)C%14)[C@H](C)O%13)/C(C)=C/C%10)(O)[C@@]%12([H])[C@H](O)C(C)=C%11)=O)C9)O[C@@H]8C(C)C
Synonyms

Abamectin; Avermectin B1a-Avermectin B1b mixt.
Structure Classification
- Antibiotics
- Macrolide Antibiotics
- Antibiotics
- Animal Husbandry
Initial Source
- Microorganisms
Streptomyces avermitilis
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (5)

All Cell Proliferation/Viability AssayCell Imaging/StainingFlow CytometryIFWB

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Journal Impact Factor

Mol Pharm

Machine Learning Models Identify New Inhibitors for Human OATP1B1. [Abstract]

2022 Nov 7;19(11):4320-4332. PMID: 36269563
J Biochem Mol Toxicol

ROS accumulation contributes to abamectin-induced apoptosis and autophagy via the inactivation of PI3K/AKT/mTOR pathway in TM3 Leydig cells. [Abstract]

2020 Aug;34(8):e22505. PMID: 32275808
J Biochem Mol Toxicol

Abamectin induces apoptosis and autophagy by inhibiting reactive oxygen species-mediated PI3K/AKT signaling in MGC803 cells. [Abstract]

2019 Jul;33(7):e22336. PMID: 30958899

Avermectin B1 purchased from MedChemExpress. Usage Cited in: J Biochem Mol Toxicol. 2019 Jul;33(7):e22336. [Abstract]

MTS assays were carried out to assess cell viability inhibition after MGC803 cells were treated with Avermectin B1 (ABA) (1, 2, 4, 8, and 16 μM) for 24 and 48 hours.

Avermectin B1 purchased from MedChemExpress. Usage Cited in: J Biochem Mol Toxicol. 2019 Jul;33(7):e22336. [Abstract]

Dose‐dependent effect of ABA on MGC803 cells morphology. MGC803 cells were treated with Avermectin B1 (ABA) for 24 hours and examined under a light microscope at ×200 magnification.

Avermectin B1 purchased from MedChemExpress. Usage Cited in: J Biochem Mol Toxicol. 2019 Jul;33(7):e22336. [Abstract]

Representative flow cytometry histograms following PI staining for cell‐cycle analysis after treatment with Avermectin B1 (ABA) (0, 1, 2, 3, and 4 μM) for 24 hours.

Avermectin B1 purchased from MedChemExpress. Usage Cited in: J Biochem Mol Toxicol. 2019 Jul;33(7):e22336. [Abstract]

The LC3 and DAPI staining detected MGC803 cell autophagy treated with Avermectin B1 (ABA) (0, 1, 2, 3, and 4 μM).

Avermectin B1 purchased from MedChemExpress. Usage Cited in: J Biochem Mol Toxicol. 2019 Jul;33(7):e22336. [Abstract]

Protein expression of LC3‐Ⅰ, LC3‐II, and P62 treated with Avermectin B1 (ABA) (0, 1, 2, 3, and 4 μM).
Virology

Evaluating anti-viral effect of Ivermectin on porcine epidemic diarrhea virus and analyzing the related genes and signaling pathway by RNA-seq in vitro. [Abstract]

2023 Oct:587:109877. PMID: 37688922
J Insect Physiol

Multi-omics analysis reveals AaaaNAT1 as a critical regulator of physiological homeostasis in Aedes aegypti. [Abstract]

2026 Mar:169:104956. PMID: 41722763

Solvant et solubilité

In Vitro:

DMSO : 250 mg/mL (286.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1454 mL

5.7268 mL

11.4536 mL

5 mM

0.2291 mL

1.1454 mL

2.2907 mL

10 mM

0.1145 mL

0.5727 mL

1.1454 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (2.38 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (291 KB)
SDS (623 KB)
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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. [1] Yiran Liang, et al. Abamectin induces cytotoxicity via the ROS, JNK, and ATM/ATR pathways. PLoS One. Environ Sci Pollut Res Int. 2020 Apr;27(12):13726-13734. [Content Brief]

[2]. Nicola Sasanelli, et al. Abamectin Efficacy on the Potato Cyst Nematode Globodera pallida. Plants (Basel). 2019 Dec 19;9(1):12. [Content Brief]

[3]. Yi Huang, et al. Cytotoxicity induced by abamectin exposure in haemocytes of Chinese mitten crab, Eriocheir sinensis. Environ Toxicol Pharmacol. 2020 Jul;77:103384. [Content Brief]

[4]. Shanshan Zhu, et al. Abamectin induces apoptosis and autophagy by inhibiting reactive oxygen species-mediated PI3K/AKT signaling in MGC803 cells. J Biochem Mol Toxicol. 2019 Jul;33(7):e22336. [Content Brief]

[5]. Mogg TD, et al. Efficacy of avermectin B1 given orally against equine intestinal strongyles and Onchocera microfilaria. Aust Vet J. 1990 Nov;67(11):399-401. [Content Brief]

[6]. Peyami Sari, et al. Pharmacokinetics of Abamectin/Levamisole Combination in a Medium Chain Mono and Diglyceride-Based Vehicle and an In Vitro Release and In Vitro In Vivo Correlation Study for Levamisole. AAPS PharmSciTech. 2017 May;18(4):1254-1260. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1454 mL	5.7268 mL	11.4536 mL	28.6339 mL
	5 mM	0.2291 mL	1.1454 mL	2.2907 mL	5.7268 mL
	10 mM	0.1145 mL	0.5727 mL	1.1454 mL	2.8634 mL
	15 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9089 mL
	20 mM	0.0573 mL	0.2863 mL	0.5727 mL	1.4317 mL
	25 mM	0.0458 mL	0.2291 mL	0.4581 mL	1.1454 mL
	30 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9545 mL
	40 mM	0.0286 mL	0.1432 mL	0.2863 mL	0.7158 mL
	50 mM	0.0229 mL	0.1145 mL	0.2291 mL	0.5727 mL
	60 mM	0.0191 mL	0.0954 mL	0.1909 mL	0.4772 mL
	80 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.3579 mL
	100 mM	0.0115 mL	0.0573 mL	0.1145 mL	0.2863 mL

Avermectin B1

5 Cited Publications

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Avermectin B1

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Publications Citing Use of MedChemExpress (MCE) Avermectin B1

Voir tous les produits spécifiques à Isoform Parasite

Voir tous les produits spécifiques à Isoform Antibiotic

Activité biologique

Chemical Information

Publications (5)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Avermectin B1 Related Classifications

HY-15311 Related Classifications