Coelonin
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Coelonin is a dihydrophenanthrene with anti-inflammation activity. Coelonin inhibits LPS-induced PTEN phosphorylation. Coelonin inhibits NF-κB activation and p27Kip1 degradation by regulating the PI3K/AKT pathway negatively. Coelonin can inhibit IκBα phosphorylation and degradation and increases the expression of IκBα protein.
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- Pureté : 98.0%
- CAS No.: 82344-82-9
- Formule: C15H14O3
- Masse moléculaire:242.27
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Stockage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
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Akt |
NF-κB |
IL-1β |
IL-6 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | EC50 |
5.05 μg/mL
Compound: 10
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Cytotoxicity against human A549 cells after 3 days by sulforhodamine B assay
Cytotoxicity against human A549 cells after 3 days by sulforhodamine B assay
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[PMID: 19193043] |
| BV-2 | IC50 |
15.2 μM
Compound: 16
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Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess assay
Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess assay
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[PMID: 31415170] |
| DU-145 | EC50 |
4.79 μg/mL
Compound: 10
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Cytotoxicity against human DU145 cells after 3 days by sulforhodamine B assay
Cytotoxicity against human DU145 cells after 3 days by sulforhodamine B assay
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[PMID: 19193043] |
| HCT-8 | EC50 |
3.84 μg/mL
Compound: 10
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Cytotoxicity against human HCT8 cells after 3 days by sulforhodamine B assay
Cytotoxicity against human HCT8 cells after 3 days by sulforhodamine B assay
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[PMID: 19193043] |
| KB | EC50 |
3.4 μg/mL
Compound: 10
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Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay
Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay
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[PMID: 19193043] |
| MCF7 | EC50 |
1.8 μg/mL
Compound: 10
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Cytotoxicity against human MCF7 cells after 3 days by sulforhodamine B assay
Cytotoxicity against human MCF7 cells after 3 days by sulforhodamine B assay
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[PMID: 19193043] |
| Neutrophil | IC50 |
>10 μM
Compound: 15
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Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as elastase substrate preincubated for 5 mins followed by fMLP/CB-induction
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as elastase substrate preincubated for 5 mins followed by fMLP/CB-induction
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[PMID: 27525452] |
| Neutrophil | IC50 |
0.4 μM
Compound: 15
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Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/cytochalasin B-induced superoxide anion generation by measuring superoxide dismutase-inhibitable reduction of ferricytochrome c preincubated for 5 mins followed by FMLP/cytochal
Antiinflammatory activity in human neutrophils assessed as inhibition of FMLP/cytochalasin B-induced superoxide anion generation by measuring superoxide dismutase-inhibitable reduction of ferricytochrome c preincubated for 5 mins followed by FMLP/cytochal
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[PMID: 27525452] |
| PC-3 | EC50 |
2.82 μg/mL
Compound: 10
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Cytotoxicity against human PC3 cells after 3 days by sulforhodamine B assay
Cytotoxicity against human PC3 cells after 3 days by sulforhodamine B assay
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[PMID: 19193043] |
In Vitro
Coelonin (2.5 μg/mL) significantly reduces both NF-κB p65 and p105/50 phosphorylation levels[1].
Coelonin (0-5 μg/mL, 1.5 h) dose dependently reduces the increase of the phosphorylation of PTEN, AKT and IκBa induced by LPS[1].
Coelonin (10 and 20 μg/ml) mitigates particulate matter 2.5 (PM2.5)-induced inflammation by reducing the generation of inflammatory factors, including interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)[2].
The inhibition of IL-1β, IL-6 and TNF-α expression by Coelonin is independent of PTEN, whereas the inhibition of p27Kip1 degradation results in cell-cycle arrest in the G1 phase, which is dependent on PTEN[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7 cells
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Concentration:0, 1, 2.5, and 5 μg/mL
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Incubation Time:1.5 h
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Result:Dose dependently reduced the increase of p65 accumulation in the nucleus induced by LPS. Dose dependently reversed LPS-induced iNOS and COX2 expression. LPS (200 ng/mL) significantly increased the phosphorylation of PTEN, AKT and inhibitor of NF-κB (IκBa), which was dose-dependently reduced by coelonin pre-treatment.
Chemical Information
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CAS No. 82344-82-9
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Appearance Solid
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Masse moléculaire 242.27
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Formule C15H14O3
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Color Light yellow to light brown
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SMILES
OC(C=C1CC2)=CC(OC)=C1C3=C2C=C(O)C=C3
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Pureté et documentation
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Fiche technique (273 KB)
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SDS (536 KB)
- English - EN (536 KB)
- Français - FR (536 KB)
- Deutsch - DE (536 KB)
- Norwegian - NO (536 KB)
- Español - ES (536 KB)
- Swedish - SV (536 KB)
- Italian - IT (536 KB)
- Korean - KR (536 KB)
- Portuguese - PT (536 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Jiang F, et al. Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism. Int J Mol Sci. 2019 Sep 8;20(18):4422. [Content Brief]
[2]. Cheng W, et al. Inhibition of inflammation-induced injury and cell migration by coelonin and militarine in PM2.5-exposed human lung alveolar epithelial A549 cells. Eur J Pharmacol. 2021 Apr 5;896:173931. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Coelonin Related Classifications
HY-N8884 Related Classifications
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