Thalidomide 5-fluoride
Based on 1 Customer Validation
Thalidomide 5-fluoride is a thalidomide-based Cereblon ligand used to recruit Cereblon protein. Thalidomide 5-fluoride can be linked to target protein ligands (e.g. IRAK4) through a linker to form PROTAC molecules (e.g. PROTAC IRAK4 degrader-1). PROTAC IRAK4 degrader-1 caused <20%, >20-50%, and >50% IRAK4 protein degradation in OCI-LY-10 cells at concentrations of 0.01, 0.1, and 1 μM, respectively.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.96%
- CAS No.: 835616-61-0
- Formule: C13H9FN2O4
- Masse moléculaire:276.22
-
Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Pureté et documentation
- Références
-
Help & FAQs
-
HY-41547
Thalidomide 4-fluorideLigands for E3 Ligase -
HY-23095
Thalidomide-5-OHLigands for E3 Ligase -
HY-103596
Thalidomide-4-OHCereblon Ligand -
HY-W039116
Thalidomide-4-BrBochemical Reagent -
HY-A0003
LenalidomideImmunomodulator -
HY-42424
(S,R,S)-AHPC-Me hydrochlorideLigands for E3 Ligase -
HY-14658
ThalidomideCereblon Inhibitor -
HY-10984
PomalidomideImmunomodulatory Agent -
HY-23095R
Thalidomide-5-OH (Standard)Ligands for E3 Ligase -
HY-W093272
Thalidomide-5,6-FLigand for E3 Ligase -
HY-139539
Thalidomide-5-COOHLigand for E3 Ligase -
HY-103596R
Thalidomide-4-OH (Standard)Reference Standard
Activité biologique
Description
IC50 & Target
|
Cereblon |
KRas G12D |
In Vitro
Chemical Information
-
CAS No. 835616-61-0
-
Appearance Solid
-
Masse moléculaire 276.22
-
Formule C13H9FN2O4
-
SMILES
O=C1N(C(CC2)C(NC2=O)=O)C(C3=C1C=CC(F)=C3)=O
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 50 mg/mL (181.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
-
Fiche technique (274 KB)
-
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
-
Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6203 mL | 18.1015 mL | 36.2030 mL | 90.5076 mL |
| 5 mM | 0.7241 mL | 3.6203 mL | 7.2406 mL | 18.1015 mL | |
| 10 mM | 0.3620 mL | 1.8102 mL | 3.6203 mL | 9.0508 mL | |
| 15 mM | 0.2414 mL | 1.2068 mL | 2.4135 mL | 6.0338 mL | |
| 20 mM | 0.1810 mL | 0.9051 mL | 1.8102 mL | 4.5254 mL | |
| 25 mM | 0.1448 mL | 0.7241 mL | 1.4481 mL | 3.6203 mL | |
| 30 mM | 0.1207 mL | 0.6034 mL | 1.2068 mL | 3.0169 mL | |
| 40 mM | 0.0905 mL | 0.4525 mL | 0.9051 mL | 2.2627 mL | |
| 50 mM | 0.0724 mL | 0.3620 mL | 0.7241 mL | 1.8102 mL | |
| 60 mM | 0.0603 mL | 0.3017 mL | 0.6034 mL | 1.5085 mL | |
| 80 mM | 0.0453 mL | 0.2263 mL | 0.4525 mL | 1.1313 mL | |
| 100 mM | 0.0362 mL | 0.1810 mL | 0.3620 mL | 0.9051 mL |
Thalidomide 5-fluoride Related Classifications
HY-W087383 Related Classifications
Powered by Bioz