2. Epigenetics TGF-beta/Smad Cell Cycle/DNA Damage
  3. PKC DNA/RNA Synthesis
  4. CGP-53353

CGP-53353 (DAPH-7) is an potent PKC inhibitor with IC50s of 0.41 μM and 3.8 μM for PKCβII and PKCβI, respectively. CGP-53353 can inhibit glucose-induced cell proliferation and DNA synthesis in AoSMC and A10 cells. CGP-53353 can be used for researching atherosclerosis of diabetic patients.

For research use only. We do not sell to patients.

CGP-53353 Chemical Structure

CGP-53353 Chemical Structure

CAS No. : 145915-60-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 176 In-stock
Solution
10 mM * 1 mL in DMSO USD 176 In-stock
Solid
5 mg USD 160 In-stock
10 mg USD 250 In-stock
25 mg USD 550 In-stock
50 mg USD 950 In-stock
100 mg USD 1550 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

CGP-53353 (DAPH-7) is an potent PKC inhibitor with IC50s of 0.41 μM and 3.8 μM for PKCβII and PKCβI, respectively. CGP-53353 can inhibit glucose-induced cell proliferation and DNA synthesis in AoSMC and A10 cells. CGP-53353 can be used for researching atherosclerosis of diabetic patients[1].

IC50 & Target[1]

PKCβII

0.41 mM (IC50)

PKCβI

3.8 mM (IC50)

In Vitro

CGP-53353 (DAPH-7) (1 μM; 48-96 hours) inhibits glucose-induced cell proliferation in A10 cells[1].
CGP-53353 (1 μM ; 0-48 hours) inhibits the glucose-induced increase and acceleration of DNA synthesis in A10, also blocks the glucose-induced increase of S-phase cell percentage[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CGP-53353 Related Antibodies

Cell Proliferation Assay

Cell Line: A10 (cell proliferation induced by glucose)[1]
Concentration: 1 μM
Incubation Time: 48-96 hours
Result: Inhibited glucose-induced cell proliferation, although A10 cell proliferation was stimulated by increasing glucose concentrations.
Molecular Weight

365.33

Formula

C20H13F2N3O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1NC(C2=C1C=C(NC3=CC=C(F)C=C3)C(NC4=CC=C(F)C=C4)=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (136.86 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7373 mL 13.6863 mL 27.3725 mL
5 mM 0.5475 mL 2.7373 mL 5.4745 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7373 mL 13.6863 mL 27.3725 mL 68.4313 mL
5 mM 0.5475 mL 2.7373 mL 5.4745 mL 13.6863 mL
10 mM 0.2737 mL 1.3686 mL 2.7373 mL 6.8431 mL
15 mM 0.1825 mL 0.9124 mL 1.8248 mL 4.5621 mL
20 mM 0.1369 mL 0.6843 mL 1.3686 mL 3.4216 mL
25 mM 0.1095 mL 0.5475 mL 1.0949 mL 2.7373 mL
30 mM 0.0912 mL 0.4562 mL 0.9124 mL 2.2810 mL
40 mM 0.0684 mL 0.3422 mL 0.6843 mL 1.7108 mL
50 mM 0.0547 mL 0.2737 mL 0.5475 mL 1.3686 mL
60 mM 0.0456 mL 0.2281 mL 0.4562 mL 1.1405 mL
80 mM 0.0342 mL 0.1711 mL 0.3422 mL 0.8554 mL
100 mM 0.0274 mL 0.1369 mL 0.2737 mL 0.6843 mL
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CGP-53353 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CGP-53353145915-60-2DAPH-7CGP53353CGP 53353DAPH7DAPH 7PKCDNA/RNA SynthesisProtein kinase CVSMCCell proliferationGlucoseDiabetesInhibitorinhibitorinhibit

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