L67
Based on 2 publication(s) in Google Scholar
L67 (DNA Ligase Inhibitor) is a competitive DNA ligase inhibitor that effectively inhibits DNA ligases I/III (both IC50 are 10 μM). L67 can cause nuclear DNA damage by reducing levels of mitochondrial DNA and increasing levels of mitochondrially-generated ROS. L67 also activates the Caspase 1-dependent apoptosis pathway in cancer cells, can be used in cancer research.
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- Reinheit : 98.0%
- CAS. Nr.: 325970-71-6
- Formel: C16H14Br2N4O4
- Molecular Weight:486.11
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) L67
MoreAlle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen
MoreAlle Caspase Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Beschreibung
|
DNA Ligase |
DNA ligases I 10 μM (IC50) |
DNA ligases III 10 μM (IC50) |
Caspase-1 |
In Vitro
L67 (10, 15 μM; 24 h) promotes nuclear DNA damage and (0-50 μM) increases the level of mSOX by inhibiting mitochondrial LigIIIα in HeLa cells[1].
L67 (10 μM; 24 h) induces changes in mitochondrial function that cause a reduction in OCR and mitochondrial DNA, and abnormal mitochondrial morphology in HeLa[1].
L67 (10, 100 μM; 24 h) induces apoptosis in cancer cells[1].
L67 (0-30 μM; 24 h) selectively induces cell death in cancer cells by activating a caspase 1-dependent apoptotic pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:10, 15 μM; 0-50 μM
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Incubation Time:24 h
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Result:Increased the formation of nuclear γH2AX foci and steady state levels of γH2AX when at 10 or 15 μM. (γH2AX: a sign of DNA double-strand breaks).
Resulted in a concentration (0-50 μM)-dependent increase in mSOX (mitochondrial superoxide) levels. (mSOX is a major cause of the cellular oxidative damage).
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Cell Line:HeLa cells
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Concentration:10 μM
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Incubation Time:24 h
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Result:Reduced oxygen consumption rate (OCR) approximately 20%.
Resulted in about a 25% reduction in mitochondrial DNA.
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Cell Line:HeLa cells
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Concentration:10, 100 μM
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Incubation Time:24 h
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Result:Result:Induced apoptosis, and at 100 μM with apoptotic cells constituting about 50% of the HeLa cell population.
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Cell Line:HeLa cells
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Concentration:0-30 μM
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Incubation Time:24 h
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Result:Activates a caspase 1-dependent cell death pathway in cancer cells.
Chemical Information
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CAS. Nr. 325970-71-6
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Appearance Solid
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Molecular Weight 486.11
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Formel C16H14Br2N4O4
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Color Light yellow to yellow
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SMILES
BrC1=CC(NCC(N/N=C/C2=CC([N+]([O-])=O)=CC=C2O)=O)=CC(Br)=C1C
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Synonyms
DNA Ligase Inhibitor
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Nat Methods
2023 Sep;20(9):1388-1399. PMID: 37474806 -
Trends Biotechnol
Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion. [Abstract]2025 Apr 7:S0167-7799(25)00083-6. PMID: 40199626
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (205.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (701 KB)
- English - EN (701 KB)
- Français - FR (701 KB)
- Deutsch - DE (701 KB)
- Norwegian - NO (701 KB)
- Español - ES (701 KB)
- Swedish - SV (701 KB)
- Italian - IT (701 KB)
- Korean - KR (701 KB)
- Portuguese - PT (701 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Sallmyr A, et al. Inhibiting Mitochondrial DNA Ligase IIIα Activates Caspase 1-Dependent Apoptosis in Cancer Cells. Cancer Res. 2016 Sep 15;76(18):5431-41. [Content Brief]
[2]. Chen X, et al. Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair. Cancer Res. 2008 May 1;68(9):3169-77. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0571 mL | 10.2857 mL | 20.5715 mL | 51.4287 mL |
| 5 mM | 0.4114 mL | 2.0571 mL | 4.1143 mL | 10.2857 mL | |
| 10 mM | 0.2057 mL | 1.0286 mL | 2.0571 mL | 5.1429 mL | |
| 15 mM | 0.1371 mL | 0.6857 mL | 1.3714 mL | 3.4286 mL | |
| 20 mM | 0.1029 mL | 0.5143 mL | 1.0286 mL | 2.5714 mL | |
| 25 mM | 0.0823 mL | 0.4114 mL | 0.8229 mL | 2.0571 mL | |
| 30 mM | 0.0686 mL | 0.3429 mL | 0.6857 mL | 1.7143 mL | |
| 40 mM | 0.0514 mL | 0.2571 mL | 0.5143 mL | 1.2857 mL | |
| 50 mM | 0.0411 mL | 0.2057 mL | 0.4114 mL | 1.0286 mL | |
| 60 mM | 0.0343 mL | 0.1714 mL | 0.3429 mL | 0.8571 mL | |
| 80 mM | 0.0257 mL | 0.1286 mL | 0.2571 mL | 0.6429 mL | |
| 100 mM | 0.0206 mL | 0.1029 mL | 0.2057 mL | 0.5143 mL |
L67 Related Classifications
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