Chuanbeinone
Chuanbeinone (22-Epidelavinone) is an orally active alkaloid found in Fritillaria pallidiflora. Chuanbeinone shows cytotoxicity against mutiple cancer cells and can induces apoptosis and S phase arrest. Chuanbeinone downregulates Bcl-2, upregulates Bax, and activates caspase-3. Chuanbeinone exerts anti-inflammatory and antitussive effects by reducing pro-inflammatory cytokine (IL-1β, IL-6 and TNF-α) production and mRNA expression, and inhibiting TRIF-, MyD88-, NF-κB-, and MAPK-dependent signaling pathways. Chuanbeinone inhibits AChE and BChE with IC50 values of 7.7 and 0.7 μM. Chuanbeinone can be used for the researches of lung carcinoma, cough, inflammatory diseases.
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- CAS. Nr.: 103530-47-8
- Formel: C27H43NO2
- Molecular Weight:413.64
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
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BChE 0.7 μM (IC50) |
AChE 7.7 μM (IC50) |
Bax |
Bcl-2 |
Caspase 3 |
IL-1β |
IL-6 |
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Cell Line
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Type | Value | Description | References |
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| A549 | CC50 |
6.8 μM
Compound: 5
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Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
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[PMID: 38294199] |
| NCI-H460 | CC50 |
25 μM
Compound: 5
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Cytotoxicity against human NCI-H460 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 method
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[PMID: 38294199] |
Chuanbeinone inhibits the growth of LLC, A2780, HepG2, and A549 cells with IC50 values of 10.51 μg/mL, 18.16 μg/mL, 37.97 μg/mL, and 43.74 μg/mL, respectively[1].
Chuanbeinone (5-15 μg/mL; 48 h) induces dose-dependent apoptosis in LLC cells, with apoptotic rates of 12.8% to 64.8%[1].
Chuanbeinone (10 μg/mL; 24-72 h) induces time-dependent S phase arrest and decreases the cells in the G0/G1 phase[1].
Chuanbeinone (5-15 μg/mL; 48 h) downregulates Bcl-2, upregulates Bax, and activates caspase-3 in LLC cells[1].
Chuanbeinone (20-100 μM; 24 h) reduces RAW 264.7 cell viability[2].
Chuanbeinone (5-20 μM; 24 h, co-treated with 0.2 μg/mL LPS) exerts anti-inflammatory effects on LPS (HY-D1056)-induced RAW 264.7 cells by reducing pro-inflammatory cytokine production and mRNA expression, and inhibiting TRIF-, MyD88-, NF-κB-, and MAPK-dependent signaling pathways[2].
Chuanbeinone inhibits AChE with an IC50 of 7.7 ± 0.001 μM and BChE with an IC50 of 0.7 ± 0.001 μM, and exhibits 70.7 ± 3.3% enzyme inhibition at 100
μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LLC
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Concentration:10 μg/mL
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Incubation Time:24, 48, 72 h
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Result:Caused time-dependent accumulation of LLC cells in the S phase, accompanied by a decrease in G0/G1 phase cells.
Increased the sub-G1 population (apoptotic DNA fragmentation) over time.
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Cell Line:LLC
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Concentration:5, 10, 15 μg/mL
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Incubation Time:48 h
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Result:Reduced expression of antiapoptotic Bcl-2 and increased expression of proapoptotic Bax (elevating the Bax/Bcl-2 ratio).
Increased levels of cleaved caspase-3 in a dose-dependent manner.
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Cell Line:RAW 264.7 cells
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Concentration:5, 10, 20 μg/mL
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Incubation Time:24 h co-treated with 0.2 μg/mL LPS
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Result:Reduced NO, IL-1β, IL-6 and TNF-α production and mRNA expression.
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Cell Line:RAW 264.7 cells
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Concentration:5, 10, 20 μg/mL
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Incubation Time:24 h co-treated with 0.2 μg/mL LPS
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Result:Reduced TRIF, MyD88, NF-κB, and MAPK levels.
Chuanbeinone (1.5-3.0 mg/kg; p.o.; single dose) exhibits dose-dependent antitussive effects in ammonia-induced cough in Mus musculus, with 3.0 mg/kg producing a 60.49% inhibition of cough frequency[4].
Chuanbeinone (3.0 mg/kg; p.o.; single dose) does not exhibit significant expectorant activity in the tracheal phenol red secretion model in mice[4].
Chuanbeinone (1.5-3.0 mg/kg; p.o.; single dose) exhibits anti-inflammatory effects at 3.0 mg/kg in xylene-induced ear edema in Mus musculus[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:LLC-bearing C57BL/6J and S180-bearing ICR mice (male, 18-22 g)[1]
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Dosage:10, 20, 40 mg/kg
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Administration:P.o.; daily; 10 days
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Result:Achieved 40.97% and 39.15% tumor growth inhibition rate.
Increased TUNEL-positive apoptotic cell number in tumor tissues.
Reduced microvessel density via decreased CD31 expression.
Increased cleaved caspase-3 expression in tumor tissues compared to the control group.
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Animal Model:Kunming mice (either sex, 18-22 g)[4]
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Dosage:1.5, 3.0 mg/kg
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Administration:P.o.; single dose
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Result:Increased cough latent period to 58.20 ± 10.64 s at 3.0 mg/kg.
Reduced cough frequency to 24.30 ± 4.84 (60.49% inhibition) at 3.0 mg/kg.
Reduced cough frequency to 30.16 ± 5.29 (52.68% inhibition) at 1.5 mg/kg.
Chemical Information
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CAS. Nr. 103530-47-8
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Molecular Weight 413.64
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Formel C27H43NO2
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SMILES
C[C@@]12[C@]3([H])[C@](CC([C@@]1([H])C[C@H](CC2)O)=O)([H])[C@@]4([H])[C@@]([C@@]5([H])[C@@]([C@H]([C@]6([H])N(C[C@H](CC6)C)C5)C)([H])CC4)([H])C3
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Synonyms
22-Epidelavinone
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
[1]. Wang D, et al. Characterization of the Isosteroidal Alkaloid Chuanbeinone from Bulbus of Fritillaria pallidiflora as Novel Antitumor Agent In Vitro and In Vivo. Planta Med. 2016;82(3):195-204. [Content Brief]
[2]. Aga EB, et al. Four Isosteroidal Alkaloids from Fritillaria Alleviate Lipopolysaccharide-Induced Inflammation in vitro and in vivo through MyD88- and TRIF-Dependent Signaling Pathways. Chin J Integr Med. 2026 Jan;32(1):54-63. [Content Brief]
[3]. Lin BQ, et al. Inhibitors of acetylcholine esterase in vitro--screening of steroidal alkaloids from Fritillaria species. Planta Med. 2006 Jul;72(9):814-8. [Content Brief]
[4]. Wang D, et al. Antitussive, expectorant and anti-inflammatory alkaloids from Bulbus Fritillariae Cirrhosae. Fitoterapia. 2011;82(8):1290-1294. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Chuanbeinone Related Classifications
HY-107272 Related Classifications
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