Cat. No.: HY-N3651

Curzerenone

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Based on 1 publication(s) in Google Scholar

Curzerenone is an orally active sesquiterpene compound and Antibacterial agent. Curzerenone can be isolated from Curcuma zedoaria and Curcuma aeruginosa plants. Curzerenone increases ROS levels, activates Apoptotic signaling pathways, and attenuates the PI3K/AKT/mTOR signaling pathway. Curzerenone exhibits anticancer activity against liver cancer and cervical cancer. Curzerenone has antioxidant effects. Curzerenone shows weak antibacterial activity against Escherichia coli. Curzerenone can be used in research related to hepatocellular carcinoma, cervical cancer, and Escherichia coli infection.

For research use only. We do not sell to patients.

Purity: 98.55%
CAS No.: 20493-56-5
Formula: C₁₅H₁₈O₂
Molecular Weight:230.30

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Curzerenone Chemical Structure

CAS No.: 20493-56-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Anti-bacteria (E. coli)^[1]

All PI3K Isoforms :

All Akt Isoforms :

All mTOR Isoforms :

In Vitro

Curzerenone (0.5-25 µM; 24 h) exhibits dose-dependent, tumor-selective cytotoxicity against hepatocellular carcinoma HepG2 cells, but causes no damage to normal renal epithelial Vero cells^[1].
Curzerenone (7.5 µM; 24 h) increases the intracellular ROS level in human hepatocellular carcinoma HepG2 cells, and this result is confirmed by DCFH-DA fluorescence assay^[1].
Curzerenone (10-1000 μM; 15 min) potently inhibits TMEM16A-mediated I⁻influx in FRT cells stably transfected with TMEM16A^[2].
Curzerenone (10-200 μM) inhibits Eact-induced Ca²⁺-activated Cl⁻currents in FRT cells stably transfected with TMEM16A in a dose-dependent manner, with a concentration of 200 μM required for complete inhibition^[2].
Curzerenone (3.06-100 μM; 24 h) reduces the survival rate of cervical cancer HeLa and SiHa cells by approximately 40%, while inhibiting cell proliferation, migration and invasion, and promoting cell apoptosis^[3].
Curzerenone (60 min) scavenges DPPH free radicals in cell-free assays, with an IC₅₀ value of 1.563 mg/mL^[4].
Curzerenone (24 h) produces a 10.8 mm inhibition zone against planktonic Escherichia coli MTCC 443 in the 24-hour disk diffusion assay^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

human liver cancer HepG2 cells, normal kidney epithelial Vero cells
Concentration:

0.5-25 µM
Incubation Time:

24 h
Result:

Induced 3% cell death in HepG2 cells at 0.5 µM.
Induced 17% cell death in HepG2 cells at 2.5 µM.
Induced 26% cell death in HepG2 cells at 5 µM.
Induced 48% cell death in HepG2 cells at 7.5 µM (the IC₅₀ dose).
Induced 55% cell death in HepG2 cells at 10 µM.
Induced 63% cell death in HepG2 cells at 25 µM.
Induced only 14.5% cell death in Vero cells at 25 µM, showing minimal cytotoxicity toward normal cells.

In Vivo

Curzerenone (80 mg/kg; p.o.; daily) inhibits the growth of cervical cancer xenografts, reduces the expression levels of PD-L1 and Ki67, increases the contents of TNF-α and IFN-γ in tumors, and inhibits the activation of NF-κB; whereas overexpression of CSNK2B reverses the above effects^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (4-week-old female, specific pathogen-free)^[3]
Dosage:

80 mg/kg
Administration:

p.o.; daily
Result:

Reduced weekly xenograft tumor volumes compared to controls.
Lowered final tumor weight at 8 weeks significantly compared to controls.
Reduced CSNK2B, PD-L1, and Ki67 staining in tumor tissues.
Elevated concentrations of TNF-α and IFN-γ in tumor homogenates.
Reduced phosphorylation of p65 and p50/p105 (markers of NF-κB activation) in tumor tissues.
Diminished all aforementioned effects in mice receiving Curzerenone plus CSNK2B-overexpressing HeLa cells.

Chemical Information

CAS No. 20493-56-5
Appearance Liquid (Density: 1.063±0.06 g/cm³)
Molecular Weight 230.30
Formula C₁₅H₁₈O₂
Color Colorless to light yellow
SMILES

O=C1C2=C(OC=C2C)C[C@@](C=C)(C)[C@H]1C(C)=C
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Hum Cell

Curzerenone inactivates the nuclear factor-kappa B signaling to suppress malignancy and immune evasion in cervical cancer by targeting CSNK2B. [Abstract]

2024 Dec 24;38(1):35. PMID: 39718697

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (217.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3422 mL

21.7108 mL

43.4216 mL

5 mM

0.8684 mL

4.3422 mL

8.6843 mL

10 mM

0.4342 mL

2.1711 mL

4.3422 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.25 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tian Y, et al. Sesquiterpene Curzerenone Activates Apoptotic Signaling and Attenuates PI3K/AKT/mTOR Signaling in HepG2 Cells by Triggering Oxidative Stress[J]. Pharmacognosy Magazine, 2026, 22(1): 395-406.

[2]. Zhu X, et al. Inhibitory activities of curzerenone, curdione, furanodienone, curcumol and germacrone on Ca²⁺-activated chloride channels. Fitoterapia. 2020;147:104736. [Content Brief]

[3]. Sun Y, et al. Curzerenone inactivates the nuclear factor-kappa B signaling to suppress malignancy and immune evasion in cervical cancer by targeting CSNK2B. Hum Cell. 2024;38(1):35. Published 2024 Dec 24. [Content Brief]

[4]. Joshi SC, et al. Antioxidant and antibacterial activities of the leaf essential oil and its constituents furanodienone and curzerenone from Lindera pulcherrima (Nees.) Benth. ex hook. f. Pharmacognosy Res. 2012 Apr;4(2):80-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.3422 mL	21.7108 mL	43.4216 mL	108.5541 mL
	5 mM	0.8684 mL	4.3422 mL	8.6843 mL	21.7108 mL
	10 mM	0.4342 mL	2.1711 mL	4.3422 mL	10.8554 mL
	15 mM	0.2895 mL	1.4474 mL	2.8948 mL	7.2369 mL
	20 mM	0.2171 mL	1.0855 mL	2.1711 mL	5.4277 mL
	25 mM	0.1737 mL	0.8684 mL	1.7369 mL	4.3422 mL
	30 mM	0.1447 mL	0.7237 mL	1.4474 mL	3.6185 mL
	40 mM	0.1086 mL	0.5428 mL	1.0855 mL	2.7139 mL
	50 mM	0.0868 mL	0.4342 mL	0.8684 mL	2.1711 mL
	60 mM	0.0724 mL	0.3618 mL	0.7237 mL	1.8092 mL
	80 mM	0.0543 mL	0.2714 mL	0.5428 mL	1.3569 mL
	100 mM	0.0434 mL	0.2171 mL	0.4342 mL	1.0855 mL

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Curzerenone

Anti-Infection Compound Library

Apoptosis Compound Library

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Publications Citing Use of MedChemExpress (MCE) Curzerenone

All PI3K Isoforms

All Akt Isoforms

All mTOR Isoforms

Biological Activity

Chemical Information

Publications (1)