2. Membrane Transporter/Ion Channel Neuronal Signaling PI3K/Akt/mTOR
  3. Piezo Channel Calcium Channel mTOR PI3K Akt
  4. D-GsMTx4

D-GsMTx4 is a spider peptide and the D enantiomer of GsMTx4 (HY-P1410). D-GsMTx4 inhibits the mechanosensitive ion channel Piezo2. D-GsMTx4 inhibits [Ca2+]i elevation. D-GsMTx4 inhibits mTOR and PI3K-Akt signaling pathways. D-GsMTx4 inhibits mechanical allodynia and thermal hyperalgesia. D-GsMTx4 can be used in researches of mechanical stress, chronic pain and idiopathic pulmonary fibrosis.

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Based on 3 publication(s) in Google Scholar

Other Forms of D-GsMTx4:

D-GsMTx4 Related Antibodies

Top Publications Citing Use of Products

    D-GsMTx4 purchased from MedChemExpress. Usage Cited in: Am J Physiol Lung Cell Mol Physiol. 2025 Aug 1;329(2):L296-L306.  [Abstract]

    D-GsMTx4 (1 μM). The short-term effect of stretch in fASM cell proliferation. fASM cells on Flexcell plates were pretreated with piezo inhibitors for 1h and then were exposed to 5% static stretch with 5% oscillatory stretch (5% CPAP) with continuous presence of the inhibitors. Control cells were exposed to 5% oscillatory stretch only. Cells were harvested after 24h treatment and counted.

    D-GsMTx4 purchased from MedChemExpress. Usage Cited in: Am J Physiol Lung Cell Mol Physiol. 2025 Aug 1;329(2):L296-L306.  [Abstract]

    D-GsMTx4 (1 μM). The protein expressions of Ki67 in fASM cells were determined by JESS.

    D-GsMTx4 purchased from MedChemExpress. Usage Cited in: Am J Physiol Lung Cell Mol Physiol. 2025 Aug 1;329(2):L296-L306.  [Abstract]

    D-GsMTx4 (1 μM). The protein expressions of PCNA in fASM cells were determined by JESS.

    D-GsMTx4 purchased from MedChemExpress. Usage Cited in: Am J Physiol Lung Cell Mol Physiol. 2025 Aug 1;329(2):L296-L306.  [Abstract]

    D-GsMTx4 (1 μM). The protein expressions of Cyclin E1 in fASM cells were determined by JESS.

    D-GsMTx4 purchased from MedChemExpress. Usage Cited in: Am J Physiol Lung Cell Mol Physiol. 2025 Aug 1;329(2):L296-L306.  [Abstract]

    The long-term effect of stretch in fASM cell proliferation. fASM cells on Flexcell plates were pretreated with D-GsMTx4 (1 μM) for 1h and then were exposed to 5% static stretch with 5% oscillatory stretch (5% CPAP) with or without the inhibitors. Control cells were exposed to 5% oscillatory stretch only. Cells were harvested after 24h treatment and seeded into 96-well plates. After 3-day culture without stretch, cell proliferation was measured by CyQUANT assay.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    D-GsMTx4 is a spider peptide and the D enantiomer of GsMTx4 (HY-P1410). D-GsMTx4 inhibits the mechanosensitive ion channel Piezo2. D-GsMTx4 inhibits [Ca2+]i elevation. D-GsMTx4 inhibits mTOR and PI3K-Akt signaling pathways. D-GsMTx4 inhibits mechanical allodynia and thermal hyperalgesia. D-GsMTx4 can be used in researches of mechanical stress, chronic pain and idiopathic pulmonary fibrosis[1][2][3][4].

    In Vitro

    D-GsMTx4 (5-10 µM) dose-dependently and reversibly inhibits Piezo2-mediated mechanosensitive inward currents in QGP-1 cells and human Piezo2-transfected HEK-293 cells[1].
    D-GsMTx4 decreases phosphorylation levels of proteins in the mTOR and PI3K-Akt signaling pathways in primary human lung fibroblasts (HLFs)[2].
    D-GsMTx4 (5 µM) inhibits mechanical stimulation-evoked [Ca2+]i increases in cultured DRG neurons from CYP-induced cystitis rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    D-GsMTx4 Related Antibodies
    In Vivo

    D-GsMTx4 (10 μg/10 μL; s.c.; 1 h after IL-1β injection) significantly inhibits mechanical allodynia and thermal hyperalgesia in the IL-1β-induced orofacial inflammatory pain rat model[4].
    D-GsMTx4 (5-10 μg/50 μL; s.c. or intracisternally) significantly reduces mechanical allodynia in the orofacial neuropathic pain rat model induced by inferior alveolar nerve injury[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Sprague Dawley rats with IL-1β-induced inflammatory pain[4].
    Dosage: 10 μg/10 μL (normal saline)
    Administration: Subcutaneous injection, 1 h after IL-1β injection
    Result: Significantly blocked IL-1β-induced mechanical allodynia and thermal hyperalgesia, with antiallodynic effects lasting 3-7 h.
    Molecular Weight

    4095.84

    Formula

    C185H273N49O45S6
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    d-(Gly-Cys-Leu-Glu-Phe-Trp-Trp-Lys-Cys-Asn-Pro-Asn-Asp-Asp-Lys-Cys-Cys-Arg-Pro-Lys-Leu-Lys-Cys-Ser-Lys-Leu-Phe-Lys-Leu-Cys-Asn-Phe-Ser-Phe)-NH2 (Disulfide bridge:Cys2-Cys17, Cys9-Cys23, Cys16-Cys30)
    Sequence Shortening

    d-(GCLEFWWKCNPNDDKCCRPKLKCSKLFKLCNFSF)-NH2 (Disulfide bridge:Cys2-Cys17, Cys9-Cys23, Cys16-Cys30)
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (24.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.2442 mL 1.2208 mL 2.4415 mL
    5 mM 0.0488 mL 0.2442 mL 0.4883 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
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    Purity & Documentation

    Purity: 99.44%

    SDS (252 KB)

    COA (272 KB) RP-HPLC (112 KB) MS (215 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.2442 mL 1.2208 mL 2.4415 mL 6.1038 mL
    5 mM 0.0488 mL 0.2442 mL 0.4883 mL 1.2208 mL
    10 mM 0.0244 mL 0.1221 mL 0.2442 mL 0.6104 mL
    15 mM 0.0163 mL 0.0814 mL 0.1628 mL 0.4069 mL
    20 mM 0.0122 mL 0.0610 mL 0.1221 mL 0.3052 mL
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    D-GsMTx4 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    D-GsMTx4Piezo ChannelCalcium ChannelmTORPI3KAktCa2+ channelsCa channelsMammalian target of RapamycinPhosphoinositide 3-kinasePKBProtein kinase BMSCsmechanical stressInhibitorinhibitorinhibit

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