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SP600125

612 Cited Publications
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Based on 612 publication(s) in Google Scholar

SP600125 is an orally active, reversible, and ATP-competitive JNK inhibitor with IC50s of 40, 40 and 90 nM for JNK1, JNK2 and JNK3, respectively. SP600125 is a potent ferroptosis inhibitor. SP600125 induces the transformation of bladder cancer cells from autophagy to apoptosis.

For research use only. We do not sell to patients.

  • Purity: 99.46%
  • CAS No.: 129-56-6
  • Formula: C14H8N2O
  • Molecular Weight:220.23
  • Storage:
    Powder   -20°C, 3 years , 4°C, 2 years ; In solvent   -80°C, 2 years , -20°C, 1 year
  • SP600125 Chemical Structure
    SP600125 Chemical Structure
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
  • Experimental Validation Results for SP600125
CAS No.: 129-56-6
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  • SP600125 Chemical Structure
    SP600125 Chemical Structure
    1/16
  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Adv Biol (Weinh). 2025 Jun 4:e00761. [Abstract]

    Customer Validation
     Macrophages were pretreated with inhibitors of the ERK (HY‐112287; ERK1/2 inhibitor 1), JNK (HY‐12041; SP600125), p38 MAPK (HY‐12839; p38 MAPK-IN-1), and p65 NF‐kB (HY‐138537; NF-κB-IN-1) pathways prior to incubation with pyroptotic‐CM for 4 h.The MET formation by macrophages was evaluated with SYTOX Green staining and detected by flow cytometry.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Adv Biol (Weinh). 2025 Jun 4:e00761. [Abstract]

    Customer Validation
     Macrophages were pretreated with inhibitors of the ERK (HY‐112287; ERK1/2 inhibitor 1), JNK (HY‐12041; SP600125), p38 MAPK (HY‐12839; p38 MAPK-IN-1), and p65 NF‐kB (HY‐138537; NF-κB-IN-1) pathways prior to incubation with pyroptotic‐CM for 4 h. The p‐p38 and p‐p65 levels in macrophages were measured by western blotting.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Mar 25;9(1):74. [Abstract]

    Customer Validation
     SP600125 (10 μM; 12 h) can significantly reduce the phosphorylation level of c-JNK stimulated by E protein (50 μg/mL; 12 h) in BEAS-2B cells.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2024 Jan 20;10(1):5. [Abstract]

    Customer Validation
     Percentage cell proliferation of CT26 cells treated with MEK inhibitor U0126-EtOH (HY-12031; 25 µM), JNK inhibitor SP600125 (HY-12041; 25 µM), P38/MAPK inhibitor SB202190 (HY-10295; 20 µM), GSK3β inhibitor TWS119 (HY-10590; 20 µM), or AR-A014418 (HY-10512; 20 µM), and vehicle or Cori.ST1911. Cell OD was measured using the CCK8 method and normalised to cell proliferation of the control. Inhibitor treatment started 4 h before Cori.ST1911 treatment. Data are represented as mean ± SEM (n = 3 independent tests), analysed by one-way analysis of variance (ANOVA) with Bonferroni’s post-hoc test. n.s., no significance.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2023 Nov 25;10(1):56. [Abstract]

    Customer Validation
     Western blotting was used to determine the level of p-IKK, IKK, p-IĸBα, IĸBα, p-p65, p65, p-MLK3, p-MKK7, p-JNK and JNK in GPR65-KO, GPR65-overexpressed, various pH-treated and GPR65 agonist/inhibitor-treated HMs. The specific inhibitors of JNK, SP600125 (10 μmol/L) and JNK-IN-8 (5 μmol/L), as well as the specific inhibitors of NF-κB, BAY 11–7082 (5 μmol/L) and APDC (20 μmol/L), were used to treat GPR65-overexpressed HMs for 24 h.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Nat Metab. 2023 Mar;5(3):481-494. [Abstract]

    Customer Validation
     Mt2 gene expression in beige adipocytes treated with indicated compounds (50 ng ml−1 TNF, 2 μM BMS345541, 1 μM SCH772984, 5 μM SB202190 and 5 μM SP600125; n = 4 biological independent samples).

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Cytotherapy. 2023 Jul;25(7):728-738. [Abstract]

    Customer Validation
     SP600125 (10 μM; 24 h) decreases the protein levels of p-JNK and p-c-Jun in THP-1 and U937 cells.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2020 Sep;331:113374. [Abstract]

    Customer Validation
     Effects of SP600125 attenuated p-JNK and RIPK3-mediated necroptosis after I/R injury. Representative western blot images and quantification of the protein expression of p-JNK, IL-6, pRIPK3 and AIF in the group pretreated with SP600125 before I/R.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2019 Apr 1;51(4):365-374. [Abstract]

    Customer Validation
     MAPKs inhibitors suppress LPS-induced COX-2 expression and AKT1 phosphorylation. RAW264.7 cells are pretreated for 1 h with U0126, SB202190, SP600125 alone, or all the three inhibitors, respectively, and then exposed to 40 ng/ml LPS for 30 min or 12 h. The phosphorylated protein kinases and COX-2 expression are detected by western blot analysis using their corresponding antibodies.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):3898-3910. [Abstract]

    Customer Validation
     Cells pretreated with SP600125 show a decreased proapoptotic Bax expression and increase antiapoptotic Bcl-2 formation when JNK pathway is blocked. Inhibition of p38 by SB202190 significantly enhanced Bcl-2 expression. Pretreatment with BAY 11-7082 to inhibit NF‐κB markedly enhance Baxm and decrease Bcl-2 expression compared with the ACR-treated group.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Jun 15;15(1):184. [Abstract]

    Customer Validation
     Representative immunoblots of total lysates from BV2 cells treated with MPP+or/and U0126 (10 μM), SP600125 (SP, 10 μM) and SB203580 (SB, 10 μM) using the antibodies against DICER.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291. [Abstract]

    Customer Validation
     Blocking the three MAPK signaling pathways through specific inhibitors (U0126; SB202190; and SP600125) significantly decrease the infection-induced neuroinflammatory response via real-time PCR analysis.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291. [Abstract]

    Customer Validation
     Blocking the three MAPK signaling pathways through specific inhibitors (U0126; SB202190; and SP600125) significantly decrease the infection-induced neuroinflammatory response via real-time PCR analysis.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2018 Mar 15:42:152-163. [Abstract]

    Customer Validation
     Cells are pretreated with SP600125 (20 μM), SB203580 (20 μM) or U0126 (20 μM) in presence or absence of KLA, then incubated with LPS (1 μg/mL) for certain time. Cell lysates are subjected to western blot.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2018 Aug 20:124:205-213. [Abstract]

    Customer Validation
     Total and phosphorylation levels of JNK in MCF7 and MDAMB231 after SP600125 treatment at 24 h.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2018 Dec;175(23):4338-4352. [Abstract]

    Customer Validation
     Treatment of macrophages with inhibitor of p38 (SB203580) or JNK (SP600125) inhibits the synthesis of pro-IL-1β in ZFP91-overexpressing THP-1 cells.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Dec:108:1294-1302. [Abstract]

    Customer Validation
     The protein levels of IL-1β and TNF-α are sharply downregulated by the addition of inhibitors SB203580, SP600125, and U0126.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Dec:108:1294-1302. [Abstract]

    Customer Validation
     The protein level of MMP-9 is downregulated by inhibitors SB203580, SP600125, and U0126.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Dec:108:1294-1302. [Abstract]

    Customer Validation
     As for both 0 g and 3 g groups, the p-ERK1/2/ERK1/2 is notably reduced by inhibitors SB203580, SP600125, and U0126.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Dec:108:1294-1302. [Abstract]

    Customer Validation
     As for both 0 g and 3 g groups, the p-p38&p38 is downregulated by inhibitors SB203580, SP600125, and U0126.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2018 Dec:108:1294-1302. [Abstract]

    Customer Validation
     As for both 0 g and 3 g groups, the p-JNK1&JNK1 is downregulated by inhibitors SB203580, SP600125, and U0126.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Autoimmun. 2018 May:89:30-40. [Abstract]

    Customer Validation
     The protein expression of S100A7 in keratinocytes incubated with DMSO or JNK inhibitor SP600125 for 48 h after transfection with siRNA for 24 h.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2018 Mar;75(6):1117-1132. [Abstract]

    Customer Validation
     Sertoli cells (SC) are pretreated with the SP600125 for 1 h followed by a 24-h treatment with MC-LR. Expression levels of MMP-8, c-Jun, c-Fos, p-ERK, ERK, p-JNK, and JNK are determined by western blotting.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2018 Jul;75(14):2627-2641. [Abstract]

    Customer Validation
     RAW264.7 macrophages are pre-treated with the inhibitor of ERK, JNK, P38, P65, and AKT signal pathway.Western blot analyzes the non- and phosphorylation of ERK, JNK, P38, P65, and AKT.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2018 Jun 27;66(25):6317-6325. [Abstract]

    Customer Validation
     HepG2 cells are pre-incubated with 20 µM SB203580 (Adezmapimod), SP600125 and PD98059 for 1 h and then treated with tangeretin(20µM) for 24 h.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: FASEB J. 2018 May;32(5):2722-2734. [Abstract]

    Customer Validation
     Cultured L02 cells exposed to 1% BSA or 200 mM PA for 12 h are pretreated with JNK inhibitor (SP600125) for 2 h. Intracellular and released HMGB1 are analyzed.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2018 Apr 23;8(1):6379. [Abstract]

    Customer Validation
     Immunoblot analysis of lysates from Anisomycin pre-treatment cells reveals an increase of CHIP protein compared with OGD treated only cells, contrasting with a decrease in RIPK3 and p-MLKL protei. Pre-treatment cells with SP600125 results in the expected attenuation in JNK phosphorylation levels.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Mol Endocrinol. 2018 Feb;60(2):145-157. [Abstract]

    Customer Validation
     SP600125 decreases Keap1 expression through inhibition of JNK activity.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Onco Targets Ther. 2018 Nov 28:11:8435-8444. [Abstract]

    Customer Validation
     Western blot results of active caspase 3 and JNK pathway-related genes in the treatment of CON, XRCC1-ON and SP600125.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):903-909. [Abstract]

    Customer Validation
     Inactivation of JNK using SP600125 in the PTP1B overexpressing cancer cells largely restores the expression levels of Twist, vimentin and Ecadherin.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2018;46(5):1779-1792. [Abstract]

    Customer Validation
     Western blot bands of p38, phospho-p38, ZO-1, Clau-din-1, and Occludin. JNK inhibitor SP600125 and p38 inhibitor SB203580 are used.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: bioRxiv. August 2, 2018.

    Customer Validation
     AGS-EBV and B95.8 cells are pretreated with SP600125 (0, 50, and 100 nM) for 24. Equal amounts of cell lysates are prepared and western blot analysis is performed to determine the levels of total and phosphorylated ERKs, p38, and JNKs and EBV lytic proteins.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2017 Mar;24(3):492-499. [Abstract]

    Customer Validation
     LPS stimulates ICER expression via p38-CREB pathway. Effect of MAPK and IKK inhibitors on LPS-induced CREB phosphorylation in peritoneal macrophages.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2017 May 1:393:22-32. [Abstract]

    Customer Validation
     Effects of p38 MAPK inhibitor (SB203580), ERK inhibitor (U0126), JNK inhibitor (SP600125), caspase inhibitor (Z-VAD-FMK) and NAC on SGC-7901 and MGC-803 treated with DOX/VCPA combination treatment. VCPA pretreatment strategy is the same as above. SB203580 (20 μM), U0126 (10 μM), SP600125 (20 μM), Z-VAD-FMK (10 μM) and NAC (5 mM) are treated 2 h before DOX (2 μg/mL) added into the culture, respectively. MAPK pathway protein levels are determined.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2017 Dec 26;7(4):e1412910. [Abstract]

    Customer Validation
     The down-regulated phosphorylation of SAPK/JNK pathway by 10µM SP600125 is detected in RAW264.7 by Western blot. The down-regulated phosphorylation of SAPK/JNK pathway by 15mg/kg SP600125 is detected in peritoneal macrophages in vivo by Western blot.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2017 Jan;39(1):71-80. [Abstract]

    Customer Validation
     Inhibition of c-Jun N-terminal kinase (JNK) expression enhances the promoting effects of miR-214 on adipocyte differentiation and decreases p-JNK protein expression in bone marrow-derived mesenchymal stem cells (BMSCs) following the overexpression of miR-214. p-JNK protein expression is even more significantly suppressed by treatment of the cells with JNK inhibitor as shown by (A) western blot analysis and (B) statistical analysis of p-JNK protein expression.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Chem Biol Interact. 2017 Nov 1:277:62-73. [Abstract]

    Customer Validation
     Effects of silencing p53 on colistin-induced ROS production and p-JNK expression level in PC-12 cells. The protein levels of p53. The protein levels of p-JNK by western blot.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Mol Immunol. 2017 Jul:87:161-170. [Abstract]

    Customer Validation
     Effect of TLR2 on autophagy activation via ERK signaling pathway in MG-infected RAW264.7 cells. RAW264.7 cells transfected with TLR2 siRNA/control siRNA are infected with MG for 30 min prior to SP600125. The expression levels of Beclin1, LC3-II/I, p-JNK, p-ERK1/2 and p-p38 are examined by Western blot. The GAPDH level was used as the internal standard.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2017:2017:6175841. [Abstract]

    Customer Validation
     Involvements of MAPK signaling pathway in CPS-induced apoptosis in ALI cultures of sheep bronchial epithelial cells. Cells are pretreated with SP600125 (a JNK inhibitor, 20 μM) for 1 h, followed by exposure to CPS (100 ng/mL) or MO (MOI = 30) for 48 h. Cell lysates are subjected to Western blotting analysis using indicated antibodies.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2017:2017:7426458. [Abstract]

    Customer Validation
     Representative immunoblot analysis of p53, p16, p21, and retinoblastoma protein (Rb) in NP cells.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2016 Dec 20;7(51):85079-85096. [Abstract]

    Customer Validation
     IL-37b inhibits TNF-α mediated activation of the pSmad3L/c-myc pathway but potentiates pSmad3C/ p21 expression in SMMC-7721 cells. Serum-starved LV_NC and LV_IL1F7b-infected SMMC-7721 cells are incubated for 8 h in the absence or presence of 10 μM SP600125 and are then treated for 30 mins with 400 pM TNF-α. Expression of endogenous Smad3 phosphorylation, p21, and c-myc is analyzed in Western blot using specific Abs.

  • 1/16

    SP600125 purchased from MedChemExpress. Usage Cited in: J Mol Cell Cardiol. 2015 Dec;89(Pt B):268-79. [Abstract]

    Customer Validation
     Dose response of MAPK and Akt inhibitors on cardiac fibroblast-derived exosomes (Exo)-induced activation of MAPKs and Akt. Neonatal rat cardiomyocytes are treated with or without Exo (50 μg/mL), U0126, SP600125, MK-2206, and SB023580 for 20 min and subjected to Western blot analysis. The results are from 4 separate experiments.

  • SP600125