FMF-04-159-2
Based on 5 publication(s) in Google Scholar
FMF-04-159-2 is a covalent CDK14 inhibitor. FMF-04-159-2 inhibits CDK14 and CDK2 with IC50s of 39.6 nM and 256 nM in NanoBRET assay, respectively.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.48%
- No. CAS: 2364489-81-4
- Fòrmula: C28H30Cl3N7O5S
- Peso molecular:683.01
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) FMF-04-159-2
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
CDK14/Cyclin Y 39.6 nM (IC50, in NanoBRET assay) |
CDK2 256 nM (IC50, in NanoBRET assay) |
In Vitro
FMF-04-159-2 inhibits HCT116 proliferation with an IC50 of 1,144±190 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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No. CAS 2364489-81-4
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Appearance Solid
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Peso molecular 683.01
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Fòrmula C28H30Cl3N7O5S
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Color White to off-white
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SMILES
O=C(C1=NNC=C1NC(C2=C(Cl)C=C(Cl)C=C2Cl)=O)NC3CCN(S(=O)(C4=CC=CC(NC(/C=C/CN(C)C)=O)=C4)=O)CC3
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Synonyms
AKOS-04
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
IRX3-CDK14 axis promotes glioblastoma progression by regulating LRP6-mediated canonical Wnt/β-catenin pathway. [Abstract]2025 Dec 23. PMID: 41436436 -
Cell Rep
CDK14 inhibition reduces mammary stem cell activity and suppresses triple negative breast cancer progression. [Abstract]2022 Sep 13;40(11):111331. PMID: 36103813 -
Life Sci
CDK14 is regulated by IGF2BP2 and involved in osteogenic differentiation via Wnt/β-catenin signaling pathway in vitro. [Abstract]2024 Dec 1:358:123148. PMID: 39447733 -
Commun Biol
Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells. [Abstract]2024 Dec 19;7(1):1649. PMID: 39702755 -
FEBS J
CDK14 is a critical regulator of haematopoietic stem and progenitor cell maintenance and post-transplantation proliferation. [Abstract]2026 May 4. PMID: 42080702
Solvente y solubilidad
In Vitro:
DMSO : ≥ 100 mg/mL (146.41 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.05 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4641 mL | 7.3205 mL | 14.6411 mL | 36.6027 mL |
| 5 mM | 0.2928 mL | 1.4641 mL | 2.9282 mL | 7.3205 mL | |
| 10 mM | 0.1464 mL | 0.7321 mL | 1.4641 mL | 3.6603 mL | |
| 15 mM | 0.0976 mL | 0.4880 mL | 0.9761 mL | 2.4402 mL | |
| 20 mM | 0.0732 mL | 0.3660 mL | 0.7321 mL | 1.8301 mL | |
| 25 mM | 0.0586 mL | 0.2928 mL | 0.5856 mL | 1.4641 mL | |
| 30 mM | 0.0488 mL | 0.2440 mL | 0.4880 mL | 1.2201 mL | |
| 40 mM | 0.0366 mL | 0.1830 mL | 0.3660 mL | 0.9151 mL | |
| 50 mM | 0.0293 mL | 0.1464 mL | 0.2928 mL | 0.7321 mL | |
| 60 mM | 0.0244 mL | 0.1220 mL | 0.2440 mL | 0.6100 mL | |
| 80 mM | 0.0183 mL | 0.0915 mL | 0.1830 mL | 0.4575 mL | |
| 100 mM | 0.0146 mL | 0.0732 mL | 0.1464 mL | 0.3660 mL |
FMF-04-159-2 Related Classifications
HY-127104 Related Classifications
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