Referencia número: HY-N3181

Nodosin

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Nodosin is an orally active diterpenoid compound that can be isolated from Isodon serra. Nodosin can inhibit the proliferation and induce cell cycle arrest and apoptosis of tumor cells. Nodosin can also inhibit oxidative stress, inflammatory responses, and ferroptosis. Nodosin has anti-inflammatory and anti-tumor activities.

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Pureza: 98.61%
No. CAS: 10391-09-0
Fòrmula: C₂₀H₂₆O₆
Peso molecular:362.42

Almacenamiento:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Nodosin Chemical Structure

No. CAS: 10391-09-0

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Actividad biológica
- Descripciòn
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

Ver todos los productos específicos de isoformas Wnt :

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HO-2

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Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 16	Cytotoxicity against human A549 cells after 48 hrs Cytotoxicity against human A549 cells after 48 hrs	[PMID: 19697926]
A549	IC₅₀	>40 μM Compound: 9	Cytotoxicity against human A549 cells after 48 hrs by MTS assay Cytotoxicity against human A549 cells after 48 hrs by MTS assay	[PMID: 24219809]
HepG2	IC₅₀	1.92 μM Compound: 16	Cytotoxicity against human HepG2 cells after 48 hrs Cytotoxicity against human HepG2 cells after 48 hrs	[PMID: 19697926]
HL-60	IC₅₀	15 μM Compound: 9	Cytotoxicity against human HL60 cells after 48 hrs by MTS assay Cytotoxicity against human HL60 cells after 48 hrs by MTS assay	[PMID: 24219809]
K562	IC₅₀	0.54 μM Compound: 16	Cytotoxicity against human K562 cells after 48 hrs Cytotoxicity against human K562 cells after 48 hrs	[PMID: 19697926]
MCF7	IC₅₀	23.5 μM Compound: 9	Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 24219809]
RAW264.7	IC₅₀	18.4 μM Compound: 9	Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 24219809]
SMMC-7721	IC₅₀	27 μM Compound: 9	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay	[PMID: 24219809]
SW480	IC₅₀	12.1 μM Compound: 9	Cytotoxicity against human SW480 cells after 48 hrs by MTS assay Cytotoxicity against human SW480 cells after 48 hrs by MTS assay	[PMID: 24219809]
SW780	IC₅₀	9.99 μM Compound: Nodosin	Cytotoxicity against human SW780 cells assessed as reduction in cell growth by CCK8 assay Cytotoxicity against human SW780 cells assessed as reduction in cell growth by CCK8 assay	[PMID: 35976233]
T-24	IC₅₀	4.91 μM Compound: Nodosin	Cytotoxicity against human T24 cells assessed as reduction in cell growth by CCK8 assay Cytotoxicity against human T24 cells assessed as reduction in cell growth by CCK8 assay	[PMID: 35976233]
UMUC3	IC₅₀	3.45 μM Compound: Nodosin	Cytotoxicity against human UMUC3 cells assessed as reduction in cell growth by CCK8 assay Cytotoxicity against human UMUC3 cells assessed as reduction in cell growth by CCK8 assay	[PMID: 35976233]

In Vitro

Nodosin (0-30 μM; 18-72 h) can inhibit the growth of multiple tumor cell lines (the IC₅₀ values for SNU638, SK-HEP-1, A549, HCT116, and MDA-MB-231 are 7.53, 5.33, 13.73, 4.05, and 7.12 μM, respectively), and induce cell cycle arrest and apoptosis in HCT116 cells. The mechanism involves the inhibition of the Wnt/β-catenin signaling pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HCT116 cells
Concentration:

0, 7.5, 15 and 30 μM
Incubation Time:

18 h
Result:

Significantly suppressed Wnt/β-catenin target protein expressions including β-catenin, p-GSK-3β, c-Myc, and Survivin.

In Vivo

Nodosin (2-4 mg/kg; oral administration; 7 days) has an ameliorating effect in a rat model of acute kidney injury^[2].
Nodosin (100 μg/mL; tail vein injection; single dose) has a protective effect in a rat liver transplantation model. It can reduce the pathological damage of rat liver tissue, decrease the number of apoptotic hepatocytes, and promote hepatocyte proliferation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague-Dawley rats (180±20 g, 8 weeks old) treated LPS (HY-D1056) to establish the acute kidney injury model^[2]
Dosage:

2 and 4 mg/kg
Administration:

Oral administration; 7 days
Result:

Significantly decreased Serum Scr, BUN, urine NGAL, and KIM-1 levels.
Decreased the levels of kidney MDA and 4-HNE, increased the levels of antioxidant enzymes (SOD, CAT, GPx, GST) and Nrf2, NQO1, HO-1, and reduced Keap-1 mRNA levels.
Reversed excessive ferroptosis in the kidneys, as indicated by increased mRNA and protein levels of GPX4, SLC7A11, and FTH-1.
Significantly reduced the levels of inflammatory markers (TLR4, MYD88, NF-κB p65, IKKβ, IL-1β) and increased IL-10 levels.
Seduced Histopathological changes in the kidneys.

Chemical Information

No. CAS 10391-09-0
Appearance Solid
Peso molecular 362.42
Fòrmula C₂₀H₂₆O₆
Color White to off-white
SMILES

O[C@H]1[C@@]([C@]2(C(C3=C)=O)C[C@H]3C1)([H])[C@@]([C@@](CC4)([H])OC2=O)(CO5)[C@](C4(C)C)([H])[C@@H]5O
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Plants
- other families
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (344.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7592 mL

13.7961 mL

27.5923 mL

5 mM

0.5518 mL

2.7592 mL

5.5185 mL

10 mM

0.2759 mL

1.3796 mL

2.7592 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (289 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Bae ES, et al. Anti-Proliferative Activity of Nodosin, a Diterpenoid from Isodon serra, via Regulation of Wnt/β-Catenin Signaling Pathways in Human Colon Cancer Cells. Biomol Ther (Seoul). 2020 Sep 1;28(5):465-472. [Content Brief]

[2]. Su C, et al. Protective effects of nodosin against lipopolysaccharide-induced acute kidney injury through regulation of oxidative stress, inflammation, and ferroptosis in rats. Naunyn Schmiedebergs Arch Pharmacol. 2024 Oct;397(10):8009-8022 [Content Brief]

[3]. Zhou X, et al. Effect of nodosin on accelerating hepatocyte regeneration after partial liver transplantation in rats. Organ Transplantation, 2016: 132-138.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7592 mL	13.7961 mL	27.5923 mL	68.9807 mL
	5 mM	0.5518 mL	2.7592 mL	5.5185 mL	13.7961 mL
	10 mM	0.2759 mL	1.3796 mL	2.7592 mL	6.8981 mL
	15 mM	0.1839 mL	0.9197 mL	1.8395 mL	4.5987 mL
	20 mM	0.1380 mL	0.6898 mL	1.3796 mL	3.4490 mL
	25 mM	0.1104 mL	0.5518 mL	1.1037 mL	2.7592 mL
	30 mM	0.0920 mL	0.4599 mL	0.9197 mL	2.2994 mL
	40 mM	0.0690 mL	0.3449 mL	0.6898 mL	1.7245 mL
	50 mM	0.0552 mL	0.2759 mL	0.5518 mL	1.3796 mL
	60 mM	0.0460 mL	0.2299 mL	0.4599 mL	1.1497 mL
	80 mM	0.0345 mL	0.1725 mL	0.3449 mL	0.8623 mL
	100 mM	0.0276 mL	0.1380 mL	0.2759 mL	0.6898 mL

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Nodosin

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