Nicotinamide
Based on 89 publication(s) in Google Scholar
Nicotinamide is a form of vitamin B3 or niacin. Nicotinamide Hydrochloride inhibits SIRT2 activity (IC50: 2 μM). Nicotinamide also inhibits SIRT1. Nicotinamide increases cellular NAD+, ATP, ROS levels. Nicotinamide inhibits tumor growth and improves survival. Nicotinamide also has anti-HBV activity.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 98-92-0
- Formula: C6H6N2O
- Molecular Weight:122.12
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Storage:
4°C, protect from light
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Nicotinamide
More- Cancer Cell. 2024 Oct 14;42(10):1729-1746.e8. [Abstract]
- Nat Photonics. 2025 Jul 10.
- Nat Genet. 2023 Dec;55(12):2175-2188. [Abstract]
- Cell Stem Cell. 2022 Sep 1;29(9):1366-1381.e9. [Abstract]
- Mol Cell. 2025 Apr 3;85(7):1349-1365.e10. [Abstract]
- Circ Res. 2022 Aug 19;131(5):456-472. [Abstract]
- Nat Commun. 2025 Mar 5;16(1):2200. [Abstract]
- Nat Commun. 2023 Sep 22;14(1):5917. [Abstract]
- Nat Commun. 2021 Sep 20;12(1):5548. [Abstract]
- Nat Commun. 2021 Jul 9;12(1):4227. [Abstract]
- Cell Death Differ. 2023 Nov;30(11):2382-2392. [Abstract]
- Acta Pharm Sin B. 2025 Dec;15(12):6382-6398. [Abstract]
- Acta Pharm Sin B. 2025 Nov;15(11):5803-5816. [Abstract]
- Autophagy. 2020 Oct;16(10):1823-1837. [Abstract]
- Theranostics. 2019 Apr 13;9(9):2424-2438. [Abstract]
- J Adv Res. 2025 Jul 12:S2090-1232(25)00545-4. [Abstract]
- J Exp Clin Cancer Res. 2025 Feb 24;44(1):67. [Abstract]
- J Exp Clin Cancer Res. 2023 Jan 5;42(1):5. [Abstract]
- Cell Discov. 2024 Sep 17;10(1):96. [Abstract]
- Redox Biol. 2025 Aug 19:86:103834. [Abstract]
- Cell Rep Med. 2025 Feb 18;6(2):101966. [Abstract]
- Pharmacol Res. 2024 Dec 31:107573. [Abstract]
- Cancer Lett. 2024 Jun 14:597:217005. [Abstract]
- Cell Death Dis. 2021 Sep 23;12(10):865. [Abstract]
- Int J Biol Macromol. 2025 Jan 7:139619. [Abstract]
- Int J Biol Macromol. 2024 Jul 27:134120. [Abstract]
- Phytomedicine. 2025 Oct 15:148:157384. [Abstract]
- EMBO J. 2020 Sep 15;39(18):e104365. [Abstract]
- Apoptosis. 2025 Apr;30(3-4):768-783. [Abstract]
- NPJ Precis Oncol. 2025 May 16;9(1):143. [Abstract]
- Oncogene. 2024 Oct;43(43):3170-3183. [Abstract]
- Oncogene. 2020 Mar;39(11):2437-2449. [Abstract]
- Aging Cell. 2021 Oct 3;e13491. [Abstract]
- Cell Death Discov. 2022 May 17;8(1):265. [Abstract]
- Oncoimmunology. 2025 Dec;14(1):2460281. [Abstract]
- Cell Mol Life Sci. 2025 Oct 24;82(1):361. [Abstract]
- EMBO Rep. 2025 Aug;26(16):4100-4123. [Abstract]
- Cell Mol Life Sci. 2025 Apr 7;82(1):140. [Abstract]
- Cell Mol Life Sci. 2025 Apr 6;82(1):139. [Abstract]
- Ecotoxicol Environ Saf. 2024 Jul 18:282:116733. [Abstract]
- Hum Reprod. 2020 Mar 27;35(3):494-503. [Abstract]
- Hum Reprod. 2019 Jul 8;34(7):1186-1194. [Abstract]
- Cell Prolif. 2024 Apr 16:e13646. [Abstract]
- J Ginseng Res. 2022 Nov;46(6):759-770. [Abstract]
- Commun Biol. 2025 Jul 4;8(1):995. [Abstract]
- Commun Biol. 2023 Mar 2;6(1):235. [Abstract]
- PLoS Pathog. 2026 Apr 22;22(4):e1014165. [Abstract]
- PLoS Pathog. 2024 Mar 12;20(3):e1012082. [Abstract]
- Int J Mol Sci. 2022 Aug 12;23(16):9049. [Abstract]
- Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):21. [Abstract]
- Int Immunopharmacol. 2023 Jul:120:110279. [Abstract]
- J Mol Cell Cardiol. 2022 Dec:173:154-168. [Abstract]
- Molecules. 2025 Oct 14;30(20):4082. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 May 13:167904. [Abstract]
- J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]
- iScience. 2026 Jan 27;29(2):114786. [Abstract]
- iScience. 2025 Sep 9;28(10):113520. [Abstract]
- iScience. 2025 Apr 3;28(5):112344. [Abstract]
- iScience. 2024 Aug 21;27(9):110775. [Abstract]
- Am J Physiol Heart Circ Physiol. 2021 Aug 1;321(2):H353-H368. [Abstract]
- Br J Haematol. 2024 Dec;205(6):2432-2441. [Abstract]
- Cell Signal. 2024 Sep 5:111386. [Abstract]
- Cardiovasc Toxicol. 2022 Nov;22(10-11):898-909. [Abstract]
- Exp Cell Res. 2022 Dec 15;421(2):113404. [Abstract]
- J Immunol. 2022 Aug 1;209(3):476-487. [Abstract]
- Hum Cell. 2024 Nov 4;38(1):13. [Abstract]
- Am J Cancer Res. 2023 Feb 15;13(2):464-474. [Abstract]
- J Proteomics. 2024 May 24:105213. [Abstract]
- J Proteomics. 2023 May 30:280:104889. [Abstract]
- Tissue Cell. 2025 Aug:95:102884. [Abstract]
- Chin J Integr Med. 2025 Feb;31(2):118-130. [Abstract]
- Theriogenology. 2020 Dec;158:233-238. [Abstract]
- PeerJ. 2023 May 11:11:e15258. [Abstract]
- Chronobiol Int. 2025 Dec 11:1-11. [Abstract]
- STAR Protoc. 2026 Feb 19;7(1):104381. [Abstract]
- Hong Kong Polytechnic University. 2025.
- bioRxiv. 2025 Oct 14.
- bioRxiv. 2025 Aug 02.
- Res Sq. 2025 Jul 14.
- Patent. US20250129329A1.
- bioRxiv. 2025 Apr 26:2025.04.23.650241. [Abstract]
- bioRxiv. 2025 April 30.
- bioRxiv. 2025 March 24.
- SSRN. 2024 May 30.
- bioRxiv. 2023 Jun 6.
- Research Square Preprint. 2022 Feb.
- Oxid Med Cell Longev. 2021 Jan 19;2021:6610543. [Abstract]
- bioRxiv. 2020 Mar.
- Patent. US20180263995A1.
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All Endogenous Metabolite Isoforms
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Biological Activity
|
Human Endogenous Metabolite |
SIRT2 2 μM (EC50) |
SIRT1 50-180 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
>10 μM
Compound: 17
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Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay
|
[PMID: 28499733] |
Nicotinamide (0-50 mM, 24/48 h ) reduces cell number in a time-dependent and dose-dependent manner in A375 and SK-MEL-28 cells[1].
Nicotinamide (10-50 mM, 24 h ) makes A375 cells undergo accumulation in G1 phase, reduction in S phase, and increase inthe sub-G1 (apoptosis) phase[1].
Nicotinamide (1-50 mM, 6 h ) increases NAD+, ATP and ROS levels in A375 and SK-MEL-28 cells[1].
Nicotinamide Hydrochloride (0.01-20 mM, 1 h) inhibits purified SIRT2 enzymatic activity in vitro with an EC50 of 2 μM[1].
Nicotinamide (0-64 mM) inhibits HBV replication in HepAD38 and HepG2.2.15 cells[3].
Nicotinamide (10 mM, on day 13) promotes pancreatic cell differentiation from human embryonic stem cells (hESCs) through CK1 and ROCK inhibition[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A375, SK-MEL-28, mouse B16-F10 cell
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Concentration:0, 1, 20, 50 mM
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Incubation Time:24 h, 48 h
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Result:Reduced cell number in a dose-dependent manner with a strong inhibitory effect at 20mM and an almost complete effect at 50 mM.
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Cell Line:A375, SK-MEL-28
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Concentration:10, 20, 50 mM
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Incubation Time:24 h
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Result:Arrested A375 cells in G1 phase.
Nicotinamide (Intraperitoneal injection, 1800 mg/Kg, once a day, murine metastatic melanoma model) affects IFN-γ (a key mediator of cell-mediated anti-tumor immunity), increases the plasma levels of Eotaxin and IL-5, reduces the plasma levels of IL-3, IL-12, RANTES and IL-10[1].
Nicotinamide (vein injection, 0-200 mg/kg, 5 days) inhibits HBV replication in HBV-transgenic mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice (subcutaneous injected with B16-F10 cells)[1]
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Dosage:1000, 1500, 1800 mg/Kg.
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Administration:Intraperitoneal injection, 5 days per week (followed by 2-day rest) or once a day.
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Result:Inhibited tumor growth at 1500 and 1800 mg/Kg, and had no effect on the body weight. Increased the frequency of IFN-γ producing cells and modulated the protein levels of cytokines and chemokines in the plasma of tumor-bearing mice.
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Animal Model:HBV-transgenic mice[3]
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Dosage:0-200 mg/kg
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Administration:Vein injection, 5 days
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Result:Reduced serum HBV DNA.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 98-92-0
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Appearance Solid
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Molecular Weight 122.12
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Formula C6H6N2O
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Color White to off-white
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SMILES
O=C(C1=CC=CN=C1)N
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Synonyms
Niacinamide; Nicotinic acid amide
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
Publications (89)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Fusobacterium nucleatum facilitates anti-PD-1 therapy in microsatellite stable colorectal cancer. [Abstract]2024 Oct 14;42(10):1729-1746.e8. PMID: 39303724 -
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Nat Genet
Multiomic analysis of cervical squamous cell carcinoma identifies cellular ecosystems with biological and clinical relevance. [Abstract]2023 Dec;55(12):2175-2188. PMID: 37985817 -
Cell Stem Cell
Hepatic cytochrome P450 8B1 and cholic acid potentiate intestinal epithelial injury in colitis by suppressing intestinal stem cell renewal. [Abstract]2022 Sep 1;29(9):1366-1381.e9. PMID: 36055192 -
Mol Cell
Spatial control of m6A deposition on enhancer and promoter RNAs through co-acetylation of METTL3 and H3K27 on chromatin. [Abstract]2025 Apr 3;85(7):1349-1365.e10. PMID: 40101711 -
Circ Res
2022 Aug 19;131(5):456-472. PMID: 35920168 -
Nat Commun
2025 Mar 5;16(1):2200. PMID: 40038279 -
Nat Commun
SCARB2 drives hepatocellular carcinoma tumor initiating cells via enhanced MYC transcriptional activity. [Abstract]2023 Sep 22;14(1):5917. PMID: 37739936 -
Nat Commun
Isonicotinylation is a histone mark induced by the anti-tuberculosis first-line drug isoniazid. [Abstract]2021 Sep 20;12(1):5548. PMID: 34545082 -
Nat Commun
mTORC1 activity regulates post-translational modifications of glycine decarboxylase to modulate glycine metabolism and tumorigenesis. [Abstract]2021 Jul 9;12(1):4227. PMID: 34244482
Nicotinamide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jul 9;12(1):4227. [Abstract]
Nicotinamide (80 μM; 4-8 h) markedly increases GLDC K514 acetylation in U251 and U87 cells.
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Cell Death Differ
Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression. [Abstract]2023 Nov;30(11):2382-2392. PMID: 37828084
Nicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Nov;30(11):2382-2392. [Abstract]
Nicotinamide (NAM) (10 mM, 4 h) enhances RACK1 acetylation levels in OVCAR3 and SKOV3 cells in combination with Trichostatin A (TSA) (HY-15144) (0.2 μM, 4 h).
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Acta Pharm Sin B
2025 Dec;15(12):6382-6398. PMID: 41477334 -
Acta Pharm Sin B
CRTAC1 derived from senescent FLSs induces chondrocyte mitochondrial dysfunction via modulating NRF2/SIRT3 axis in osteoarthritis progression. [Abstract]2025 Nov;15(11):5803-5816. PMID: 41311393 -
Autophagy
The role of the key autophagy kinase ULK1 in hepatocellular carcinoma and its validation as a treatment target. [Abstract]2020 Oct;16(10):1823-1837. PMID: 31986961 -
Theranostics
EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. [Abstract]2019 Apr 13;9(9):2424-2438. PMID: 31131045 -
J Adv Res
SIRT7 facilitates ferroptosis resistance of melanocytes via activating the SMAD3-ATF3-GPX4 signaling pathway in vitiligo. [Abstract]2025 Jul 12:S2090-1232(25)00545-4. PMID: 40659088 -
J Exp Clin Cancer Res
CD47 blockade reverses resistance to HDAC inhibitor by liberating anti-tumor capacity of macrophages. [Abstract]2025 Feb 24;44(1):67. PMID: 39994810 -
J Exp Clin Cancer Res
KLF14 regulates the growth of hepatocellular carcinoma cells via its modulation of iron homeostasis through the repression of iron-responsive element-binding protein 2. [Abstract]2023 Jan 5;42(1):5. PMID: 36600258 -
Cell Discov
Targeting SNRNP200-induced splicing dysregulation offers an immunotherapy opportunity for glycolytic triple-negative breast cancer. [Abstract]2024 Sep 17;10(1):96. PMID: 39285160 -
Redox Biol
Oxidative stress-induced ZEB1 acetylation drives a hybrid epithelial-mesenchymal phenotype and promotes lung metastasis in triple-negative breast cancer. [Abstract]2025 Aug 19:86:103834. PMID: 40850192 -
Cell Rep Med
KRASG12D-driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC. [Abstract]2025 Feb 18;6(2):101966. PMID: 39970873 -
Pharmacol Res
YAP K236 acetylation facilitates its nucleic export and deprived the protection against cardiac hypertrophy in mice. [Abstract]2024 Dec 31:107573. PMID: 39746498
Nicotinamide purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 Dec 31:107573. [Abstract]
Nicotinamide (NAM) enhanced ISO (10 μM)-induced YAP acetylation in neonatal rat cardiomyocytes (NRCMs).
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Cancer Lett
Acetylation-dependent deubiquitinase USP26 stabilizes BAG3 to promote breast cancer progression. [Abstract]2024 Jun 14:597:217005. PMID: 38880224 -
Cell Death Dis
Fibroblast growth factor 21 (FGF21) alleviates senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the SIRT1-mTOR signaling pathway. [Abstract]2021 Sep 23;12(10):865. PMID: 34556628 -
Int J Biol Macromol
SIRT5 desuccinylating IDH2 to alleviate oxidative stress in bovine mammary epithelial cells induced by ammonia. [Abstract]2025 Jan 7:139619. PMID: 39788270 -
Int J Biol Macromol
Inhibition of SIRT1 relieves hepatocarcinogenesis via alleviating autophagy and inflammation. [Abstract]2024 Jul 27:134120. PMID: 39074701 -
Phytomedicine
Diosgenin exerts dual impacts on Th17 cell differentiation in adjuvant-induced arthritis rats by impairing SIRT1-mediated deacetylation. [Abstract]2025 Oct 15:148:157384. PMID: 41101071 -
EMBO J
2020 Sep 15;39(18):e104365. PMID: 32696520 -
Apoptosis
Brazilin alleviates acute lung injury via inhibition of ferroptosis through the SIRT3/GPX4 pathway. [Abstract]2025 Apr;30(3-4):768-783. PMID: 39720978
Nicotinamide purchased from MedChemExpress. Usage Cited in: Apoptosis. 2025 Apr;30(3-4):768-783. [Abstract]
Nicotinamide (NAM) (20 mM; pretreatment for 24 hours before LPS exposure in MLE-12 cells) increases GPX4 acetylation levels in MLE-12 cells.
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NPJ Precis Oncol
Hepatocellular carcinoma cells downregulate PGAM2 via SIRT2-mediated deacetylation modification to enhance aerobic glycolysis. [Abstract]2025 May 16;9(1):143. PMID: 40379975 -
Oncogene
USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2. [Abstract]2024 Oct;43(43):3170-3183. PMID: 39256572 -
Oncogene
Stabilization of FASN by ACAT1-mediated GNPAT acetylation promotes lipid metabolism and hepatocarcinogenesis. [Abstract]2020 Mar;39(11):2437-2449. PMID: 31974474 -
Aging Cell
Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation. [Abstract]2021 Oct 3;e13491. PMID: 34605151 -
Cell Death Discov
Acetylation stabilizes stathmin1 and promotes its activity contributing to gallbladder cancer metastasis. [Abstract]2022 May 17;8(1):265. PMID: 35581193
Nicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 May 17;8(1):265. [Abstract]
Nicotinamide (NAM) (6-8 h) upregulates stathmin1 acetylation in 293T cells transfected with stathmin1-Flag plasmids.
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Oncoimmunology
Leptin decreases Th17/Treg ratio to facilitate neuroblastoma via inhibiting long-chain fatty acid catabolism in tumor cells. [Abstract]2025 Dec;14(1):2460281. PMID: 39902867
Nicotinamide purchased from MedChemExpress. Usage Cited in: Oncoimmunology. 2025 Dec;14(1):2460281. [Abstract]
Nicotinamide (NAM) (6 h) promotes CPT2 acetylation in SK-N-AS cells.
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Cell Mol Life Sci
β-Hydroxybutyrate enhances malate dehydrogenase 2 β-hydroxybutyrylation to alleviate hepatic steatosis in MASLD. [Abstract]2025 Oct 24;82(1):361. PMID: 41134319 -
EMBO Rep
PCAF-mediated acetylation regulates RAD51 dynamic localization on chromatin during HR repair. [Abstract]2025 Aug;26(16):4100-4123. PMID: 40664718 -
Cell Mol Life Sci
Loss of NgBR causes neuronal damage through decreasing KAT7-mediated RFX1 acetylation and FGF1 expression. [Abstract]2025 Apr 7;82(1):140. PMID: 40192836 -
Cell Mol Life Sci
Single-cell transcriptomics reveals liver developmental trajectory during lineage reprogramming of human induced hepatocyte-like cells. [Abstract]2025 Apr 6;82(1):139. PMID: 40188417 -
Ecotoxicol Environ Saf
Nicotinamide improves the impaired extravillous trophoblast cell invasion induced by PM2.5 exposure-associated increase of TNFα secretion through the ROS/NF-κB/FLT1 pathway. [Abstract]2024 Jul 18:282:116733. PMID: 39029224 -
Hum Reprod
Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility. [Abstract]2020 Mar 27;35(3):494-503. PMID: 32142584
Nicotinamide purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2020 Mar 27;35(3):494-503. [Abstract]
Nicotinamide (NAM) (40 mM; 1 h) increases the global level of lysine 2-hydroxyisobutyrylation along the human sperm tail.
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Hum Reprod
2019 Jul 8;34(7):1186-1194. PMID: 31194865
Nicotinamide purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2019 Jul 8;34(7):1186-1194. [Abstract]
Nicotinamide (NAM) (20-40 mM; 1 h) increases global levels of Kac (lysine acetylation) and Kglu (lysine glutarylation) in human sperm.
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Cell Prolif
Deletion of Tgm2 suppresses BMP-mediated hepatocyte-to-cholangiocyte metaplasia in ductular reaction. [Abstract]2024 Apr 16:e13646. PMID: 38623945 -
J Ginseng Res
Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons. [Abstract]2022 Nov;46(6):759-770. PMID: 36312736 -
Commun Biol
Endometrial stromal Menin supports endometrial receptivity by maintaining homeostasis of WNT signaling pathway through H3K4me3 during WOI. [Abstract]2025 Jul 4;8(1):995. PMID: 40610728 -
Commun Biol
Bacterial PncA improves diet-induced NAFLD in mice by enabling the transition from nicotinamide to nicotinic acid. [Abstract]2023 Mar 2;6(1):235. PMID: 36864222 -
PLoS Pathog
Nicotinamide metabolism is essential for Hepatitis C Virus replication and the production of infectious Lipo-Viro-Particles. [Abstract]2026 Apr 22;22(4):e1014165. PMID: 42018568 -
PLoS Pathog
KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation. [Abstract]2024 Mar 12;20(3):e1012082. PMID: 38470932 -
Int J Mol Sci
Aberrant Retinal Pigment Epithelial Cells Derived from Induced Pluripotent Stem Cells of a Retinitis Pigmentosa Patient with the PRPF6 Mutation. [Abstract]2022 Aug 12;23(16):9049. PMID: 36012314 -
Invest Ophthalmol Vis Sci
PRPF8 Mutation-Induced Defects in Human iPSC-Derived RPE Are Rescued by Adenine Base Editing. [Abstract]2026 Jan 5;67(1):21. PMID: 41533919 -
Int Immunopharmacol
Echinacoside ameliorates 5-fluorouracil-induced endothelial injury and senescence through SIRT1 activation. [Abstract]2023 Jul:120:110279. PMID: 37187128 -
J Mol Cell Cardiol
Deletion of SIRT6 in vascular smooth muscle cells facilitates vascular calcification via suppression of DNA damage repair. [Abstract]2022 Dec:173:154-168. PMID: 36367517 -
Molecules
Anti-Aging, Anti-Inflammatory, and Cytoprotective Properties of Lactobacillus- and Kombucha-Fermented C. pepo L. Peel and Pulp Extracts with Prototype Skin Toner Development. [Abstract]2025 Oct 14;30(20):4082. PMID: 41157100 -
Biochim Biophys Acta Mol Basis Dis
METTL3 promotes human amniotic epithelial stem cells differentiation into insulin-producing cells by regulation of MaFA expression. [Abstract]2025 May 13:167904. PMID: 40374016 -
J Mol Med (Berl)
2019 Aug;97(8):1183-1193. PMID: 31201471 -
iScience
Biomimetic hydrogel for the construction of patient-derived bladder cancer organoids with aggressive growth. [Abstract]2026 Jan 27;29(2):114786. PMID: 41704753 -
iScience
ALKBH5-IGF2BP2 axis mediates prostate cancer progression and docetaxel resistance via m6A-stabilized CLSPN RNA. [Abstract]2025 Sep 9;28(10):113520. PMID: 41069850 -
iScience
2025 Apr 3;28(5):112344. PMID: 40276762 -
iScience
Global isonicotinylome analysis identified SMAD3 isonicotinylation promotes liver cancer cell epithelial-mesenchymal transition and invasion. [Abstract]2024 Aug 21;27(9):110775. PMID: 39286495 -
Am J Physiol Heart Circ Physiol
Soluble epoxide hydrolase deletion attenuated nicotine-induced arterial stiffness via limiting the loss of SIRT1. [Abstract]2021 Aug 1;321(2):H353-H368. PMID: 34142887 -
Br J Haematol
Nicotinamide enhances Treg differentiation by promoting Foxp3 acetylation in immune thrombocytopenia. [Abstract]2024 Dec;205(6):2432-2441. PMID: 39406393 -
Cell Signal
HDAC5 deacetylates c-Myc and facilitates cell cycle progression in hepatocellular carcinoma cells. [Abstract]2024 Sep 5:111386. PMID: 39243916 -
Cardiovasc Toxicol
Genistein Alleviates Oxidative Stress and Inflammation in the Hypothalamic Paraventricular Nucleus by Activating the Sirt1/Nrf2 Pathway in High Salt-Induced Hypertension. [Abstract]2022 Nov;22(10-11):898-909. PMID: 35986807 -
Exp Cell Res
Inhibition of 14-3-3ε by K50 acetylation activates YAP1 to promote cholangiocarcinoma growth. [Abstract]2022 Dec 15;421(2):113404. PMID: 36341908 -
J Immunol
Deacetylation of K481 and K484 on Penaeid Shrimp Hemocyanin Is Critical for Antibacterial Activity. [Abstract]2022 Aug 1;209(3):476-487. PMID: 35851542 -
Hum Cell
Establishment and drug resistance characterization of paired organoids using human primary colorectal cancer and matched tumor deposit specimens. [Abstract]2024 Nov 4;38(1):13. PMID: 39495391 -
Am J Cancer Res
SIRT6 drives sensitivity to ferroptosis in anaplastic thyroid cancer through NCOA4-dependent autophagy. [Abstract]2023 Feb 15;13(2):464-474. PMID: 36895980 -
J Proteomics
Global proteomic analyses of lysine acetylation, malonylation, succinylation, and crotonylation in human sperm reveal their involvement in male fertility. [Abstract]2024 May 24:105213. PMID: 38797435 -
J Proteomics
Global proteomic analysis reveals lysine succinylation contributes to the pathogenesis of aortic aneurysm and dissection. [Abstract]2023 May 30:280:104889. PMID: 36966968 -
Tissue Cell
Melatonin promotes diabetic wound healing by mediating mitochondrial function in endothelial cells through the AMPK/SIRT1/HIF-1α pathway. [Abstract]2025 Aug:95:102884. PMID: 40233668 -
Chin J Integr Med
Gentiopicroside Alleviates Atherosclerosis by Suppressing Reactive Oxygen Species-Dependent NLRP3 Inflammasome Activation in Vascular Endothelial Cells via SIRT1/Nrf2 Pathway. [Abstract]2025 Feb;31(2):118-130. PMID: 39671057 -
Theriogenology
Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model. [Abstract]2020 Dec;158:233-238. PMID: 32980686 -
PeerJ
Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection. [Abstract]2023 May 11:11:e15258. PMID: 37193023 -
Chronobiol Int
Pterostilbene regulates circadian clock gene expression in high-fat diet-induced obese mice. [Abstract]2025 Dec 11:1-11. PMID: 41379079 -
STAR Protoc
Protocol for generating human tonsil immune organoids with epithelial and germinal center layers using low-Matrigel culture. [Abstract]2026 Feb 19;7(1):104381. PMID: 41722051 -
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bioRxiv
A nuclear branched-chain amino acid catabolism pathway controls histone propionylation in pancreatic cancer. [Abstract]2025 Apr 26:2025.04.23.650241. PMID: 40568091 -
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Oxid Med Cell Longev
Protective Effects of Oroxylin A against Doxorubicin-Induced Cardiotoxicity via the Activation of Sirt1 in Mice. [Abstract]2021 Jan 19;2021:6610543. PMID: 33542782 -
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Solvent & Solubility
DMSO : 50 mg/mL (409.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 50 mg/mL (409.43 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (818.87 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Francesca Scatozza, et al. Nicotinamide inhibits melanoma in vitro and in vivo. J Exp Clin Cancer Res. 2020 Oct 7;39(1):211. [Content Brief]
[2]. Hwang ES, et al. Nicotinamide is an inhibitor of SIRT1 in vitro, but can be a stimulator in cells. Cell Mol Life Sci. 2017 Sep;74(18):3347-3362. [Content Brief]
[3]. Li WY, et al. The SIRT1 inhibitor, nicotinamide, inhibits hepatitis B virus replication in vitro and in vivo. Arch Virol. 2016 Mar;161(3):621-30. [Content Brief]
[4]. Zhang Y, et al. Nicotinamide promotes pancreatic differentiation through the dual inhibition of CK1 and ROCK kinases in human embryonic stem cells. Stem Cell Res Ther. 2021 Jun 25;12(1):362. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 8.1887 mL | 40.9433 mL | 81.8867 mL | 204.7167 mL |
| 5 mM | 1.6377 mL | 8.1887 mL | 16.3773 mL | 40.9433 mL | |
| 10 mM | 0.8189 mL | 4.0943 mL | 8.1887 mL | 20.4717 mL | |
| 15 mM | 0.5459 mL | 2.7296 mL | 5.4591 mL | 13.6478 mL | |
| 20 mM | 0.4094 mL | 2.0472 mL | 4.0943 mL | 10.2358 mL | |
| 25 mM | 0.3275 mL | 1.6377 mL | 3.2755 mL | 8.1887 mL | |
| 30 mM | 0.2730 mL | 1.3648 mL | 2.7296 mL | 6.8239 mL | |
| 40 mM | 0.2047 mL | 1.0236 mL | 2.0472 mL | 5.1179 mL | |
| 50 mM | 0.1638 mL | 0.8189 mL | 1.6377 mL | 4.0943 mL | |
| 60 mM | 0.1365 mL | 0.6824 mL | 1.3648 mL | 3.4119 mL | |
| 80 mM | 0.1024 mL | 0.5118 mL | 1.0236 mL | 2.5590 mL | |
| 100 mM | 0.0819 mL | 0.4094 mL | 0.8189 mL | 2.0472 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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