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Cat. No.: HY-189700

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JL-15-DNBS

JL-15-DNBS is an Hsp70-Bim interaction disruptor. JL-15-DNBS undergoes GSH-responsive activation to release active JL-15, and GSH depletion attenuates its cytotoxicity. JL-15-DNBS downregulates the oncogenic client proteins AKT and eIF4E. JL-15-DNBS can be used for research on chronic myeloid leukemia (CML).

For research use only. We do not sell to patients.

CAS No.: 3048341-50-7
Formula: C₃₄H₃₂ClN₅O₈S
Molecular Weight:706.16

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

JL-15-DNBS Chemical Structure

CAS No.: 3048341-50-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

HSP70

Bim

Akt

eIF4E

All HSP Isoforms :

All Bcl-2 Family Isoforms :

All Akt Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
K562	EC50	2.5 μM	Induction of apoptosis in human K562 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation. Induction of apoptosis in human K562 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation.	42732692
BV-173	EC50	1.6 μM	Induction of apoptosis in human BV173 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation. Induction of apoptosis in human BV173 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation.	42732692
HEK293	EC50	188 μM	Induction of apoptosis in human HEK293 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation. Induction of apoptosis in human HEK293 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation.	42732692
BaF3	EC50	225 μM	Induction of apoptosis in BaF3 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation. Induction of apoptosis in BaF3 cells assessed by FITC-conjugated Annexin V staining and flow cytometry after 48 hrs incubation.	42732692

In Vitro

Oxidative StressAnti-cancerApoptosisOthers

Oxidative Stress

JL-15-DNBS (100 μM; 4-6 h) is efficiently activated by 1 mM GSH in cell-free buffer, releasing active JL-15^[1].

Anti-cancer

Apoptosis

JL-15-DNBS (gradient concentrations; 48 h) selectively induces apoptosis in K562, BV173, and K562-R3 CML cells with EC₅₀ values of 2.5, 1.6, and 0.78 μM, respectively, and has a selective index of 90.0^[1].

Anti-cancer

Apoptosis

Oxidative Stress

JL-15-DNBS (gradient concentrations; 48 h) cytotoxicity in K562 cells is GSH/RSH-dependent, with BSO depletion increasing EC₅₀ to 123.5 μM while NAC has minimal effect^[1].

Anti-cancer

JL-15-DNBS (gradient concentrations; 48 h) disrupts Hsp70-Bim PPI in K562 cells with potency comparable to JL-15^[1].
JL-15-DNBS (48-96 h) downregulates AKT and eIF4E in K562 cells, consistent with efficient intracellular activation and on-target action^[1].

Others

JL-15-DNBS (300 μM) shows no detectable Hsp70 binding by ITC, indicating the masked hydroxyl is required for target engagement^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

K562 cells
Concentration:

5 μM
Incubation Time:

48 h; 96 h
Result:

Reduced AKT and eIF4E protein levels in K562 cells.

Related Protocols :

Chemical Information

CAS No. 3048341-50-7
Molecular Weight 706.16
Formula C₃₄H₃₂ClN₅O₈S
SMILES

O=S(C1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)(OC2=CC(C3=CC=C(OC4=CC(C)=C(Cl)C(C)=C4)C=C3)=CC(NCCN5CCCCC5)=C2C#N)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Sun Q, et al. Structure-guided design of a GSH-activated Hsp70 targeting prodrug with enhanced tumor selectivity. Bioorganic chemistry. 2026 Sep 09;182:110488.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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JL-15-DNBS

All HSP Isoforms

All Bcl-2 Family Isoforms

All Akt Isoforms

Biological Activity

Description

IC50 & Target

Cellular Effect

In Vitro

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

JL-15-DNBS Related Classifications

HY-189700 Related Classifications

Bioz

Keywords

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