Nummularine B
Nummularine B (Daechuine S27; N-Demethylamphibine H) is an anti-parasite agent. Nummularine B inhibits calmodulin-dependent phosphodiesterase activity with an IC50 of 16.8 μM. Nummularine B inhibits the growth of Plasmodium and Leishmania donovani in vitro. Nummularine B inhibits the calmodulin-dependent activity of actomyosin Ca2+-ATPase. Nummularine B is applicable to research related to malaria and visceral leishmaniasis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 53947-96-9
- 分子式: C32H41N5O6
- 分子量:591.71
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
体外実験
Nummularine B inhibits the growth of the K1 strain of Plasmodium falciparum, with an IC50 of 3.6 μM[1].
Nummularine B (20 min) inhibits calmodulin-dependent Ca2+-ATPase activity in actomyosin from rat posterior thigh muscle, with an IC50 of 27.0 μM[2].
Nummularine B (10 min) inhibits the activity of calmodulin-dependent phosphodiesterase from bovine brain-derived enzymes, with an IC50 value of 16.8 μM[2].
Nummularine B (Compound 11) (72 h (T. b. rhodesiense, L. donovani, P. falciparum); 96 h (T. cruzi)) potently inhibits Plasmodium falciparum with an IC50 of 14.7 μM; it also inhibits Leishmania donovani with an IC50 of 44.8 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 53947-96-9
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分子量 591.71
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分子式 C32H41N5O6
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SMILES
C([C@@H](NC([C@@H](NC)C)=O)C(C)C)(=O)N1[C@]2([C@@](OC=3C=C(C(OC)=CC3)/C=C\NC(=O)[C@H](CC4=CC=CC=C4)NC2=O)(CC1)[H])[H]
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別名
Daechuine S27; N-Demethylamphibine H
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配列
{Me-Ala}-Val-Cyclo({N-[(1Z)-2-(5-hydroxy-2-methoxyphenyl)ethenyl]-Pro}-Phe) (lactam bridge: Pro3-Phe4)
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シーケンスの短縮
{Me-Ala}-V-Cyclo({N-[(1Z)-2-(5-hydroxy-2-methoxyphenyl)ethenyl]-Pro}-F) (lactam bridge: Pro3-Phe4)
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Structure Classification
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
純度とドキュメンテーション
参考文献
[1]. Tuenter E, et al. Antiplasmodial Activity, Cytotoxicity and Structure-Activity Relationship Study of Cyclopeptide Alkaloids. Molecules. 2017;22(2):224. Published 2017 Feb 2. [Content Brief]
[2]. Hwang KH, et al. Inhibition of calmodulin-dependent calcium-ATPase and phosphodiesterase by various cyclopeptides and peptide alkaloids from the Zizyphus species. Arch Pharm Res. 2001;24(3):202-206. [Content Brief]
[3]. Sevik Kilicaslan O, et al. Antiprotozoal activity of natural products from Nigerien plants used in folk medicine. Front Pharmacol. 2023;14:1190241. Published 2023 Jun 23. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Nummularine B 関連分類
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