製品番号: HY-N2156

Paeonolide

(別名: ペオノリド)

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Based on 1 Customer Validation

Paeonolide, found in Paeonia suffruticosa, is an ERK1/2 activator. Paeonolide promotes early and late osteoblast differentiation, stimulates pre-osteoblast transmigration, and activates the BMP-Smad1/5/8, Wnt-β-catenin, JNK and p38 pathways. Paeonolide can be used for the research of osteoporosis, periodontitis.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 99.58%
CAS 番号: 72520-92-4
分子式: C₂₀H₂₈O₁₂
分子量:460.43

保管条件:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Paeonolide 構造式

CAS 番号: 72520-92-4

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

IC₅₀ & Target^[1]

ERK1

ERK2

ERK アイソフォーム固有の製品をすべて表示 :

TGF-beta/Smad アイソフォーム固有の製品をすべて表示 :

Smad1
Smad3

Wnt アイソフォーム固有の製品をすべて表示 :

JNK アイソフォーム固有の製品をすべて表示 :

p38 MAPK アイソフォーム固有の製品をすべて表示 :

体外実験

Paeonolide (0.1-100 µM; 24 h) does not affect viability or proliferation of MC3T3-E1 pre-osteoblasts^[1].
Paeonolide (1-30 µM; 24 h) enhances wound healing migration of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner^[1].
Paeonolide (1-30 µM; 7 days) promotes early osteoblast differentiation of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner, as measured by increased ALP staining and activity^[1].
Paeonolide (1-30 µM; 14 days) promotes late osteoblast differentiation and mineralized nodule formation of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner, as measured by increased ARS staining and osteoblast-specific gene expression^[1].
Paeonolide (1-10 µM; 24 h) activates the BMP-Smad1/5/8, Wnt-β-catenin, and MAPK (ERK1/2, JNK, p38) signaling pathways in OS-induced MC3T3-E1 pre-osteoblasts, with a dominant effect on ERK1/2 phosphorylation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MC3T3-E1 pre-osteoblasts
Concentration:

0.1, 1, 10, 30, and 100 µM
Incubation Time:

24 h
Result:

Showed no cytotoxic or proliferative effects across all tested concentrations, with cell viability remaining near 100% relative to untreated controls.

Cell Migration Assay ^[1]

Cell Line:

OS-induced MC3T3-E1 pre-osteoblasts
Concentration:

1, 10, 30 µM
Incubation Time:

24 h
Result:

Promoted wound healing in a dose-dependent manner.
Showed statistically significant increases compared to OS-only controls at all concentrations.

Cell Differentiation Assay^[1]

Cell Line:

OS-induced MC3T3-E1 pre-osteoblasts
Concentration:

1, 10, 30 µM
Incubation Time:

7 days
Result:

Promoted early osteoblast differentiation in a dose-dependent manner, with increased ALP staining intensity and ALP-positive cell counts observed via microscopy.
Showed statistically significant increases compared to OS-only controls at all concentrations.

Western Blot Analysis^[1]

Cell Line:

OS-induced MC3T3-E1 pre-osteoblasts
Concentration:

1, 10 µM
Incubation Time:

24 h
Result:

Increased BMP2 protein expression and phosphorylation of Smad1/5/8 in a dose-dependent manner.
Enhanced Wnt3a protein expression, phosphorylation of GSK3β, and β-catenin protein levels in a dose-dependent manner.
Increased phosphorylation of ERK1/2, JNK, and p38 in a dose-dependent manner.
Promoted phosphorylation of ERK1/2 to a greater extent than BMP2 (100 ng/mL) treatment.

化学情報

CAS 番号 72520-92-4
性状 Solid
分子量 460.43
分子式 C₂₀H₂₈O₁₂
Color White to off-white
SMILES

O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1CO[C@H](OC[C@H](O)[C@@H]2O)[C@@H]2O)OC(C=C(OC)C=C3)=C3C(C)=O
別名

ペオノリド
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (217.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1719 mL

10.8594 mL

21.7188 mL

5 mM

0.4344 mL

2.1719 mL

4.3438 mL

10 mM

0.2172 mL

1.0859 mL

2.1719 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Park KR, et al. Paeonolide as a Novel Regulator of Core-Binding Factor Subunit Alpha-1 in Bone-Forming Cells. Int J Mol Sci. 2021;22(9):4924. Published 2021 May 6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1719 mL	10.8594 mL	21.7188 mL	54.2971 mL
	5 mM	0.4344 mL	2.1719 mL	4.3438 mL	10.8594 mL
	10 mM	0.2172 mL	1.0859 mL	2.1719 mL	5.4297 mL
	15 mM	0.1448 mL	0.7240 mL	1.4479 mL	3.6198 mL
	20 mM	0.1086 mL	0.5430 mL	1.0859 mL	2.7149 mL
	25 mM	0.0869 mL	0.4344 mL	0.8688 mL	2.1719 mL
	30 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8099 mL
	40 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3574 mL
	50 mM	0.0434 mL	0.2172 mL	0.4344 mL	1.0859 mL
	60 mM	0.0362 mL	0.1810 mL	0.3620 mL	0.9050 mL
	80 mM	0.0271 mL	0.1357 mL	0.2715 mL	0.6787 mL
	100 mM	0.0217 mL	0.1086 mL	0.2172 mL	0.5430 mL

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Paeonolide

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Mass Spectrometry Natural Product Library

ERK アイソフォーム固有の製品をすべて表示

TGF-beta/Smad アイソフォーム固有の製品をすべて表示

Wnt アイソフォーム固有の製品をすべて表示

JNK アイソフォーム固有の製品をすべて表示

p38 MAPK アイソフォーム固有の製品をすべて表示

生物活性

化学情報

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Paeonolide 関連分類

HY-N2156 関連分類