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Cat. No.: HY-183575

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JQ1-JX5

JQ1-JX5 is a DCAF16-based BRD4 PROTAC degrader. JQ1-JX5 covalently modifies Cys58 of DCAF16, promotes ternary complex formation with BRD4, enables BRD4 ubiquitination and proteasomal degradation. JQ1-JX5 induces time-dependent degradation of BRD4 long and short isoforms in AGS cells with DC₅₀ of 43.97 and 16.77 nM. JQ1-JX5 can be used for the research of cancer, such as acute myeloid leukemia.
(Pink: BRD4 ligand (HY-78695); Blue: DCAF16 ligand (HY-W005799); Black: linker).

For research use only. We do not sell to patients.

Formula: C₂₉H₂₄ClN₇O₂S₂
Molecular Weight:602.13

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

JQ1-JX5 Chemical Structure

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

JQ1-JX5 is a DCAF16-based BRD4 PROTAC degrader. JQ1-JX5 covalently modifies Cys58 of DCAF16, promotes ternary complex formation with BRD4, enables BRD4 ubiquitination and proteasomal degradation. JQ1-JX5 induces time-dependent degradation of BRD4 long and short isoforms in AGS cells with DC₅₀ of 43.97 and 16.77 nM. JQ1-JX5 can be used for the research of cancer, such as acute myeloid leukemia^[1]. (Pink: BRD4 ligand (HY-78695); Blue: DCAF16 ligand (HY-W005799); Black: linker).

IC₅₀ & Target^[1]

BRD4

DCAF16

All PROTACs Isoforms :

All Epigenetic Reader Domain Isoforms :

In Vitro

JQ1-JX5 (1.0 μM; 3-36 h) induces time-dependent degradation of BRD4 long and short isoforms in AGS cells with DC₅₀ of 43.97 and 16.77 nM, and ~90% loss of both isoforms achieved by 24 hours^[1].
JQ1-JX5 (0.01-5 μM; 12 h) induces degradation of BRD4 long and short isoforms in AGS, MDA-MB-453, AsPC1, MV-4-11, MDA-MB-231, and HEK293T cells^[1].
JQ1-JX5 inhibits AGS cells proliferation with an IC₅₀ of 0.4993 μM^[1].
JQ1-JX5 mediates the degradation of BRD4 by covalent recruitment of DCAF16^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

AGS cancer cells
Concentration:

1.0 μM
Incubation Time:

3, 6, 9, 12, 24, 36 h
Result:

Induced time-dependent degradation of BRD4, with detectable reduction in both long and short isoforms within 3 h.
Caused ~90% loss of both BRD4 isoforms after 24 h of treatment.
Led to near-complete depletion of both BRD4 isoforms after 36 h.

Chemical Information

Molecular Weight 602.13
Formula C₂₉H₂₄ClN₇O₂S₂
SMILES

CC1=NN=C2[C@@H](N=C(C3=C(N21)SC(C)=C3C)C4=CC=C(C=C4)Cl)CC(NCCOC5=CC=C6N=C(SC6=C5)C#N)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhang J, et al. New Reversible Covalent Warheads: Cyanobenzothiazoles Recruiting DCAF16 for Targeted Protein Degradation. J Med Chem. 2026;69(9):11620-11637. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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JQ1-JX5

All PROTACs Isoforms

All Epigenetic Reader Domain Isoforms