Fucosterol
Based on 3 publication(s) in Google Scholar
Fucosterol is a sterol isolated from algae, seaweed or diatoms. Fucosterol exhibits various biological activities, including antioxidant, anti-adipogenic, blood cholesterol reducing, anti-diabetic and anti-cancer activities. Fucosterol regulates adipogenesis via inhibition of PPARα and C/EBPα expression and can be used for anti-obesity agents development research.
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- Purity : 98.27%
- CAS No.: 17605-67-3
- 화학식: C29H48O
- 분자량:412.69
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Fucosterol (Standard)
Publications Citing Use of MedChemExpress (MCE) Fucosterol
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Customer Validation & Images
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RT-PCR
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WB
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Cell Proliferation/Viability Assay
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Flow Cytometry
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IHC
All Endogenous Metabolite Isoforms
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Biological Activity
제품 설명
IC50 & Target
PPARα; C/EBPα[1]
In Vitro
Fucosterol (0-50 μM; 7 days) suppresses the expression of PPARα and C/EBPα when compared to fully differentiated control adipocytes[1]. Fucosterol shows cytotoxicity against T47D and HT29 cell lines with IC50 values of 27.94 and 70.41 μg/ml, respectively[3].Fucosterol decreases cell HEK293, MCF-7, and SiHa cells proliferation with IC50 values of 185.4, 43.3, and 34.0 μg/ml, respectively[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:3T3-L1 adipocytes
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Concentration:0 μM; 25 μM; 50 μM
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Incubation Time:7 days
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Result:Inhibited PPARα and C/EBPα expression.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 17605-67-3
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Appearance Solid
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분자량 412.69
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화학식 C29H48O
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Color White to off-white
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SMILES
C/C=C(C(C)C)/CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4CC(O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Structure Classification
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Fucosterol ameliorates depressive-like behaviours by suppressing microglial activation and neuroinflammation via inhibition the MAPK/ERK1/2 signaling pathway. [Abstract]2025 Jun 17:158:114821. PMID: 40349406 -
Atheroscler Plus
Fucosterol exerts an anti-atherosclerotic action via NF-κB and p38/Erk MAPK signaling pathways. [Abstract]2025 May 13:60:51-59. PMID: 40502621
Fucosterol purchased from MedChemExpress. Usage Cited in: Atheroscler Plus. 2025 May 13:60:51-59. [Abstract]
RT-qPCR analysis of expression levels of adhesion molecules and inflammatory cytokines in mouse aorta treated with Fucosterol (100 mg/kg, i.g.)
Fucosterol purchased from MedChemExpress. Usage Cited in: Atheroscler Plus. 2025 May 13:60:51-59. [Abstract]
Western blot analysis of protein levels of adhesion molecules and inflammatory cytokines in mouse aorta treated with Fucosterol (100 mg/kg, i.g.).
Fucosterol purchased from MedChemExpress. Usage Cited in: Atheroscler Plus. 2025 May 13:60:51-59. [Abstract]
Cell viability in treated HUVECs was detected by CCK-8 assay treated with Fucosterol (50 μM).
Fucosterol purchased from MedChemExpress. Usage Cited in: Atheroscler Plus. 2025 May 13:60:51-59. [Abstract]
Flow cytometry analysis of apoptosis in treated HUVECs treated with Fucosterol (50 μM).
Fucosterol purchased from MedChemExpress. Usage Cited in: Atheroscler Plus. 2025 May 13:60:51-59. [Abstract]
Immunohistochemical staining for PCSK9 in aortic root treated with Fucosterol (100 mg/kg, i.g.).
용액&용해도
In Vitro:
Ethanol : 16.67 mg/mL (40.39 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (4.05 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Corn Oil
Solubility: 10 mg/mL (24.23 mM); Clear solution; Need ultrasonic and warming and heat to 45°C
Add each solvent one by one: 17% Solutol HS-15 in Saline
Solubility: 6.25 mg/mL (15.14 mM); Suspended solution; Need ultrasonic and warming and heat to 42°C
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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3T3-L1 preadipocyte-to-adipocyte differentiation
3T3-L1 preadipocytes are induced to differentiate after growth arrest using adipogenic media containing insulin, dexamethasone, and IBMX; differentiation is assessed by lipid-droplet accumulation, triglyceride increase, Oil Red O staining, and adipocyte-marker induction such as PPARγ and C/EBPα.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
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Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.
순도&문서
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Data Sheet (286 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Jung HA, et al. Anti-adipogenic activity of the edible brown alga Ecklonia stolonifera and its constituent fucosterol in 3T3-L1 adipocytes. Arch Pharm Res. 2014 Jun;37(6):713-20. [Content Brief]
[2]. Lee YS, et al. Anti-diabetic activities of fucosterol from Pelvetia siliquosa. Arch Pharm Res. 2004 Nov;27(11):1120-2. [Content Brief]
[3]. Qudeer Ahmed Abdul, et al. Health benefit of fucosterol from marine algae: a review. J Sci Food Agric. 2016 Apr;96(6):1856-66 [Content Brief]
[4]. Khanavi M, et al. Cytotoxicity of fucosterol containing fraction of marine algae against breast and colon carcinoma cell line. Pharmacogn Mag. 2012 Jan;8(29):60-4. [Content Brief]
[5]. Caamal-Fuentes E, et al. Cytotoxic and antiproliferative constituents from Dictyota ciliolata, Padina sanctae-crucis and Turbinaria tricostata. Pharm Biol. 2014 Oct;52(10):1244-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.4231 mL | 12.1156 mL | 24.2313 mL | 60.5782 mL |
| 5 mM | 0.4846 mL | 2.4231 mL | 4.8463 mL | 12.1156 mL | |
| 10 mM | 0.2423 mL | 1.2116 mL | 2.4231 mL | 6.0578 mL | |
| 15 mM | 0.1615 mL | 0.8077 mL | 1.6154 mL | 4.0385 mL | |
| 20 mM | 0.1212 mL | 0.6058 mL | 1.2116 mL | 3.0289 mL | |
| 25 mM | 0.0969 mL | 0.4846 mL | 0.9693 mL | 2.4231 mL | |
| 30 mM | 0.0808 mL | 0.4039 mL | 0.8077 mL | 2.0193 mL | |
| 40 mM | 0.0606 mL | 0.3029 mL | 0.6058 mL | 1.5145 mL |
Fucosterol Related Classifications
HY-N4103 Related Classifications
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