Hexamethylene bisacetamide
Based on 1 Customer Validation
Hexamethylene bisacetamide (HMBA) is a differentiation inducer and selective bromine domain inhibitor that can differentiate across the blood-brain barrier. Hexamethylene bisacetamide can induce tumor cell differentiation and inhibit cell proliferation, showing antitumor activity. Hexamethylene bisacetamide induces apoptosis by Notch1, Bcl-2 and p53 signaling pathways. In addition, Hexamethylene bisacetamide improves the obesity phenotype of mice.
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- Purity : 99.56%
- CAS No.: 3073-59-4
- 화학식: C10H20N2O2
- 분자량:200.28
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Erythroleukemia cell line | IC50 |
5 mM
Compound: Fig 5, Cpd 3
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Anticancer activity against mouse Erythroleukemia cell line assessed as inhibition of cell growth
Anticancer activity against mouse Erythroleukemia cell line assessed as inhibition of cell growth
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[PMID: 34591474] |
| HL-60 | IC50 |
4 mM
Compound: HMBA 1
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The compound was evaluated in vitro for cytotoxicity against HL-60 cell lines
The compound was evaluated in vitro for cytotoxicity against HL-60 cell lines
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10.1016/S0960-894X(01)80996-3 |
In Vitro
Hexamethylene bisacetamide (2.5-10 mM; 15 days) significantly inhibits the proliferation of SHG-44 cells in a dose-dependent manner, but the inhibitory effect is reversible[2].
Hexamethylene bisacetamide (5-10 mM; 15 days) changes the morphology of SHG-44 cells and increases the degree of cell differentiation[2].
Hexamethylene bisacetamide (1-20 mM; 30-480 minutes) inhibits the activation of MAPK, Akt signaling pathways and NF - κB in TNFα-treated A549 cells[3].
Hexamethylene bisacetamide (5 mM; 2 h-7 days) induces apoptosis through Notch1, Bcl - 2 and p53 signaling pathways in Molt4 cells[4].
Hexamethylene bisacetamide (0.1 mM; 2 h) regulates the expression of neuropeptides through MYH9 and ACTG1 in hypothalamic cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human malignant glioma cell line SHG-44
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Concentration:2.5, 5, 7.5 and 10 mM
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Incubation Time:15 days
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Result:Reduced cell proliferation compared with the control group.
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Cell Line:A549 cells
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Concentration:10 mM
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Incubation Time:30, 60, 120 and 240 min
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Result:Inhibited the phosphorylation levels of p44/p42 MAPK and MEK1/2.
Inhibited the phosphorylation levels of Akt.
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Cell Line:Molt4 cells
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Concentration:5 mM
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Incubation Time:2 h, 4 h, 8 h, 24 h, 48 h, 5 days and 7 days
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Result:Reduced the levels of Notch1.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice with diet-induced obese (DIO) model[5]
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Dosage:1,000 mg/kg (i.p.);
500 and 1,000 mg/kg (i.v.);
400 nmoles 2 μL (ICV) -
Administration:Intraperitoneal injection (i.p.); 7 days
Intravenous injection (i.v.); 7 days
Intracerebroventricular injection (ICV); 7 days -
Result:Ameliorated obese phenotype in DIO mice, reduced weight gain and food intake and improved metabolic parameters in whatever injection way.
Did not cause sickness behaviors in DIO mice in whatever injection way.
Regulate hypothalamic neuropeptide expression in whatever injection way.
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Research Protocol for Metabolic Diseases
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- Notch Pathway Solutions
- MTT Cell Proliferation Assay
- Subcutaneous Cell-Line-Derived Xenograft
- iPSC cell differentiation
Chemical Information
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CAS No. 3073-59-4
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Appearance Solid
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분자량 200.28
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화학식 C10H20N2O2
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Color White to off-white
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SMILES
CC(NCCCCCCNC(C)=O)=O
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Synonyms
HMBA
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
H2O : 100 mg/mL (499.30 mM; Need ultrasonic)
DMSO : 25 mg/mL (124.83 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Nilsson LM, et al. Muralidharan SV, Demir D, Welin M, Bhadury J, Logan DT, Walse B, Nilsson JA. Cancer Differentiating Agent Hexamethylene Bisacetamide Inhibits BET Bromodomain Proteins. Cancer Res. 2016 Apr 15;76(8):2376-83. [Content Brief]
[2]. Li XN, et al. Modulation effects of hexamethylene bisacetamide on growth and differentiation of cultured human malignant glioma cells. J Neurosurg. 1996 May;84(5):831-8. [Content Brief]
[3]. Dey A, et al. Hexamethylene bisacetamide (HMBA) simultaneously targets AKT and MAPK pathway and represses NF kappaB activity: implications for cancer therapy. Cell Cycle. 2008 Dec;7(23):3759-67. [Content Brief]
[4]. Cecchinato V, et al. Hexamethylene bisacetamide inhibits malignant phenotype in T-ALL cell lines. Leuk Res. 2008 May;32(5):791-7. https://pubmed.ncbi.nlm.nih.gov/17964649/ [Content Brief]
[5]. Park S, et al. HMBA ameliorates obesity by MYH9- and ACTG1-dependent regulation of hypothalamic neuropeptides. EMBO Mol Med. 2023 Dec 7;15(12):e18024. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.9930 mL | 24.9650 mL | 49.9301 mL | 124.8252 mL |
| 5 mM | 0.9986 mL | 4.9930 mL | 9.9860 mL | 24.9650 mL | |
| 10 mM | 0.4993 mL | 2.4965 mL | 4.9930 mL | 12.4825 mL | |
| 15 mM | 0.3329 mL | 1.6643 mL | 3.3287 mL | 8.3217 mL | |
| 20 mM | 0.2497 mL | 1.2483 mL | 2.4965 mL | 6.2413 mL | |
| 25 mM | 0.1997 mL | 0.9986 mL | 1.9972 mL | 4.9930 mL | |
| 30 mM | 0.1664 mL | 0.8322 mL | 1.6643 mL | 4.1608 mL | |
| 40 mM | 0.1248 mL | 0.6241 mL | 1.2483 mL | 3.1206 mL | |
| 50 mM | 0.0999 mL | 0.4993 mL | 0.9986 mL | 2.4965 mL | |
| 60 mM | 0.0832 mL | 0.4161 mL | 0.8322 mL | 2.0804 mL | |
| 80 mM | 0.0624 mL | 0.3121 mL | 0.6241 mL | 1.5603 mL | |
| 100 mM | 0.0499 mL | 0.2497 mL | 0.4993 mL | 1.2483 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Hexamethylene bisacetamide Related Classifications
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