Paeonolide
Based on 1 Customer Validation
Paeonolide, found in Paeonia suffruticosa, is an ERK1/2 activator. Paeonolide promotes early and late osteoblast differentiation, stimulates pre-osteoblast transmigration, and activates the BMP-Smad1/5/8, Wnt-β-catenin, JNK and p38 pathways. Paeonolide can be used for the research of osteoporosis, periodontitis.
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- Purity: 99.89%
- CAS No.: 72520-92-4
- 화학식: C20H28O12
- 분자량:460.43
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
ERK1 |
ERK2 |
In Vitro
Paeonolide (0.1-100 µM; 24 h) does not affect viability or proliferation of MC3T3-E1 pre-osteoblasts[1].
Paeonolide (1-30 µM; 24 h) enhances wound healing migration of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner[1].
Paeonolide (1-30 µM; 7 days) promotes early osteoblast differentiation of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner, as measured by increased ALP staining and activity[1].
Paeonolide (1-30 µM; 14 days) promotes late osteoblast differentiation and mineralized nodule formation of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner, as measured by increased ARS staining and osteoblast-specific gene expression[1].
Paeonolide (1-10 µM; 24 h) activates the BMP-Smad1/5/8, Wnt-β-catenin, and MAPK (ERK1/2, JNK, p38) signaling pathways in OS-induced MC3T3-E1 pre-osteoblasts, with a dominant effect on ERK1/2 phosphorylation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MC3T3-E1 pre-osteoblasts
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Concentration:0.1, 1, 10, 30, and 100 µM
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Incubation Time:24 h
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Result:Showed no cytotoxic or proliferative effects across all tested concentrations, with cell viability remaining near 100% relative to untreated controls.
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Cell Line:OS-induced MC3T3-E1 pre-osteoblasts
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Concentration:1, 10, 30 µM
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Incubation Time:24 h
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Result:Promoted wound healing in a dose-dependent manner.
Showed statistically significant increases compared to OS-only controls at all concentrations.
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Cell Line:OS-induced MC3T3-E1 pre-osteoblasts
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Concentration:1, 10, 30 µM
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Incubation Time:7 days
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Result:Promoted early osteoblast differentiation in a dose-dependent manner, with increased ALP staining intensity and ALP-positive cell counts observed via microscopy.
Showed statistically significant increases compared to OS-only controls at all concentrations.
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Cell Line:OS-induced MC3T3-E1 pre-osteoblasts
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Concentration:1, 10 µM
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Incubation Time:24 h
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Result:Increased BMP2 protein expression and phosphorylation of Smad1/5/8 in a dose-dependent manner.
Enhanced Wnt3a protein expression, phosphorylation of GSK3β, and β-catenin protein levels in a dose-dependent manner.
Increased phosphorylation of ERK1/2, JNK, and p38 in a dose-dependent manner.
Promoted phosphorylation of ERK1/2 to a greater extent than BMP2 (100 ng/mL) treatment.
Chemical Information
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CAS No. 72520-92-4
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Appearance Solid
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분자량 460.43
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화학식 C20H28O12
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Color White to off-white
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SMILES
O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1CO[C@H](OC[C@H](O)[C@@H]2O)[C@@H]2O)OC(C=C(OC)C=C3)=C3C(C)=O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
DMSO : 100 mg/mL (217.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1719 mL | 10.8594 mL | 21.7188 mL | 54.2971 mL |
| 5 mM | 0.4344 mL | 2.1719 mL | 4.3438 mL | 10.8594 mL | |
| 10 mM | 0.2172 mL | 1.0859 mL | 2.1719 mL | 5.4297 mL | |
| 15 mM | 0.1448 mL | 0.7240 mL | 1.4479 mL | 3.6198 mL | |
| 20 mM | 0.1086 mL | 0.5430 mL | 1.0859 mL | 2.7149 mL | |
| 25 mM | 0.0869 mL | 0.4344 mL | 0.8688 mL | 2.1719 mL | |
| 30 mM | 0.0724 mL | 0.3620 mL | 0.7240 mL | 1.8099 mL | |
| 40 mM | 0.0543 mL | 0.2715 mL | 0.5430 mL | 1.3574 mL | |
| 50 mM | 0.0434 mL | 0.2172 mL | 0.4344 mL | 1.0859 mL | |
| 60 mM | 0.0362 mL | 0.1810 mL | 0.3620 mL | 0.9050 mL | |
| 80 mM | 0.0271 mL | 0.1357 mL | 0.2715 mL | 0.6787 mL | |
| 100 mM | 0.0217 mL | 0.1086 mL | 0.2172 mL | 0.5430 mL |
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