Lenoremycin
Lenoremycin (Ro 21-6150) is a microbial metabolite. Lenoremycin decreases β-catenin and cyclin D1 proteins levels. Lenoremycin decreases cancer stem cells (CSCs) populations via inducing reactive oxygen species production.
For research use only. We do not sell to patients.
- CAS No.: 51257-84-2
- Formula: C47H78O13
- Molecular Weight:851.11
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
1.2 μM
Compound: 9
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Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs by resazurin assay
Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs by resazurin assay
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[PMID: 28029795] |
| HEK293 | EC50 |
38 nM
Compound: 9
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Modulation of unfolded protein response in tunicamycin-treated HEK293 cells after 48 hrs by ASGR-Cluc secretion assay
Modulation of unfolded protein response in tunicamycin-treated HEK293 cells after 48 hrs by ASGR-Cluc secretion assay
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[PMID: 28029795] |
Chemical Information
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CAS No. 51257-84-2
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Molecular Weight 851.11
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Formula C47H78O13
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SMILES
C[C@H]1[C@]2(O[C@](C[C@H]1O[C@@H]3O[C@@H]([C@H](CC3)OC)C)([H])[C@@H](C)/C=C(C)/C([C@H](C)C[C@H](C)C(O)=O)=O)O[C@@](CC2)([C@]4([H])O[C@@]5([C@@H](CC4)C)O[C@]([C@@]6([H])O[C@](O)([C@@H](C[C@@H]6C)C)CO)([H])C[C@@H]5C)C
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Synonyms
Ro 21-6150; Antibiotic A-130-A
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Structure Classification
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Initial Source
Streptomyces hygroscopicus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Lenoremycin Related Classifications
HY-129694 Related Classifications
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