2. Others Apoptosis Cell Cycle/DNA Damage
  3. Drug Derivative Apoptosis CDK ASK1
  4. MG16

MG16 is a prodrug of 10-Methoxycamptothecin (HY-N0446). MG16 downregulates CDK6 and upregulates ASK1. MG16 induces cell cycle arrest and Apoptosis. MG16 exhibits anticancer activity against Lewis lung carcinoma, small cell lung cancer, and non-small cell lung cancer.

For research use only. We do not sell to patients.

MG16

MG16 Chemical Structure

CAS No. : 2349329-01-5

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

MG16 Related Antibodies

Top Publications Citing Use of Products

View All CDK Isoform Specific Products:

View All Isoforms
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

MG16 is a prodrug of 10-Methoxycamptothecin (HY-N0446). MG16 downregulates CDK6 and upregulates ASK1. MG16 induces cell cycle arrest and Apoptosis. MG16 exhibits anticancer activity against Lewis lung carcinoma, small cell lung cancer, and non-small cell lung cancer[1].

IC50 & Target[1]

CDK6

 

In Vitro

MG16 (48 h) potently inhibits the proliferation of LLC, A549 and H446 lung cancer cells in vitro, with IC50 values of 408.2 ± 32.4, 613.1 ± 54.7 and 140.4 ± 10.4 nM[1], respectively.
Treatment with MG16 (0.1-100 nM; 48 h) induces dose-dependent apoptosis in A549 and H446 lung cancer cells, and its pro-apoptotic activity in both cell lines is significantly stronger than that of SN-38[1].
MG16 (90.1-1 μM for A549, 10-100 nM for H446; 24 h) regulates the expression of key apoptotic proteins and induces robust Caspase-3 activation in A549 and H446 lung cancer cells after 24 h of treatment, with a more prominent Caspase-3 activation effect than SN-38[1].
MG16 (613.1 nM; 48 h) induces cell cycle arrest at the G0/G1 phase in A549 lung cancer cells following 48 h of treatment at its IC50 concentration[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MG16 Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 lung cancer cells, H446 lung cancer cells
Concentration: 0.1-1 μM (A549 cells); 10-100 nM (H446 cells)
Incubation Time: 24 h
Result: Upregulated the proapoptotic protein Bax, downregulated the antiapoptotic protein Bcl-2, and strongly activated caspase-3 in A549 cells, with significantly higher cleaved caspase-3 levels than SN-38 at both tested concentrations.
Upregulated Bax, downregulated Bcl-2, and activated caspase-3 in H446 cells, with significantly higher cleaved caspase-3 levels than SN-38 at both tested concentrations, especially at 100 nM.
In Vivo

MG16 (5 mg/kg; i.v.; every other day) achieves a 75.11% tumor inhibition rate, showing potent lung cancer growth suppression in a mouse LLC subcutaneous tumor model[1].
MG16 (5 mg/kg; i.v.; every other day) achieves an 80.5% tumor inhibition rate, showing potent lung cancer growth suppression in a mouse A549 subcutaneous tumor model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

471.89

Formula

C23H22ClN3O6
CAS No.
SMILES

CC[C@]1(C(OCC2=C1C=C3C4=NC5=CC=C(C=C5C=C4CN3C2=O)OC)=O)OC(CN)=O.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

MG16 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MG162349329-01-5MG 16MG-16Drug DerivativeApoptosisCDKASK1Drug derivativeCyclin dependent kinaseApoptosis signal-regulating kinase 1MAP3K5MAPKKK5Mitogen-activated protein kinase kinase kinase 5MEK kinase 5human lung cancer xenograft mouse modelsLewis lung carcinoma cellsmitochondrial apoptotic pathwayslung cancer cellshuman lung cancer cell linesG0/G1 cell cycle arrestcyclin-dependent kinase 6G1-to-S phase cell cycle transitionsyngeneic Lewis lung carcinoma mouse modelsapoptosis signal-regulating kinase 1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
MG16
Cat. No.:
HY-182071
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.