MMP-9-IN-4
MMP-9-IN-4 is a MMP-9 inhibitor (IC50: 7.46 nM) that has H-π interactions with MMP-9. MMP-9-IN-4 also inhibits AKT activity (IC50: 8.82 nM). MMP-9-IN-4 shows cell cytotoxicity and induces cell apoptosis. MMP-9-IN-4 can be used in the research of cancers.
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- CAS No.: 2581824-39-5
- 화학식: C28H19F3N4O6
- 분자량:564.47
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
MMP-9 7.46 nM (IC50) |
MMP-1 41 nM (IC50) |
MMP-2 139 nM (IC50) |
MMP-13 113 nM (IC50) |
AKT 8.82 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
2.2 nM
Compound: 11
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Anticancer activity against human HepG2 cells assessed as reduction in cell viability by MTT assay
Anticancer activity against human HepG2 cells assessed as reduction in cell viability by MTT assay
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[PMID: 31740054] |
| MCF7 | IC50 |
3.6 nM
Compound: 11
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Anticancer activity against human MCF7 cells assessed as reduction in cell viability by MTT assay
Anticancer activity against human MCF7 cells assessed as reduction in cell viability by MTT assay
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[PMID: 31740054] |
| NFS-60 | IC50 |
3.61 nM
Compound: 11
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Anticancer activity against human NFS-60 cells assessed as reduction in cell viability by MTT assay
Anticancer activity against human NFS-60 cells assessed as reduction in cell viability by MTT assay
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[PMID: 31740054] |
| WI-38 | IC50 |
374 nM
Compound: 11
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Cytotoxicity against human WI-38 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human WI-38 cells assessed as reduction in cell viability by MTT assay
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[PMID: 31740054] |
In Vitro
MMP-9-IN-4 (compound 11, 0-1 μM approximately, 72 h) shows cytotoxicity to Wi-38, MCF-7, NFS-60, HepG-2 cells[1].
MMP-9-IN-4 (72 h) induces apoptosis in MCF-7, NFS-60 and HepG-2 cells at 3.6 nM, 3.61 nM, 2.2 nM respectively[1].
MMP-9-IN-4 (72 h) induces significant caspase 3/7 activation in MCF-7, NFS-60 and HepG-2 cells at 3.6 nM, 3.61 nM, 2.2 nM respectively [1].
MMP-9-IN-4 (2.2 nM, 24 h) inhibits cell migration in HepG-2 cells[1].
MMP-9-IN-4 has anti-bacterial activity against E.coli with a MIC value of 1.814 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Wi-38, MCF-7, NFS-60, HepG-2 cells
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Concentration:0-1 μM approximately
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Incubation Time:72 h
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Result:Showed cytotoxicity with IC50s: 374 nM (Wi-38), 3.6 nM (MCF-7), 3.61 nM (NFS-60), 2.2 nM (HepG-2).
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Cell Line:HepG-2
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Concentration:2.2 nM
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Incubation Time:24 h
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Result:Inhibited cell migration by 93.77%.
- Mitochondrial membrane-potential fluorescent assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- Mitochondrial membrane-potential and mitochondrial mass staining
- Apoptosis Solutions
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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CAS No. 2581824-39-5
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분자량 564.47
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화학식 C28H19F3N4O6
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SMILES
O=C(C(N(C1=CC=CC=C1)C(C2=CC=C(C=C2)C(F)(F)F)=O)C3=CC=C(C=C3)[N+]([O-])=O)NC4=CC=C(C=C4)[N+]([O-])=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MMP-9-IN-4 Related Classifications
HY-146217 Related Classifications
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