Cat. No.: HY-N2156

Paeonolide

Customer Review

Based on 1 Customer Validation

Paeonolide, found in Paeonia suffruticosa, is an ERK1/2 activator. Paeonolide promotes early and late osteoblast differentiation, stimulates pre-osteoblast transmigration, and activates the BMP-Smad1/5/8, Wnt-β-catenin, JNK and p38 pathways. Paeonolide can be used for the research of osteoporosis, periodontitis.

For research use only. We do not sell to patients.

Purity: 99.58%
CAS No.: 72520-92-4
Formula: C₂₀H₂₈O₁₂
Molecular Weight:460.43

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Paeonolide Chemical Structure

CAS No.: 72520-92-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

ERK1

ERK2

All ERK Isoforms :

All TGF-beta/Smad Isoforms :

Smad1
Smad3

All Wnt Isoforms :

All JNK Isoforms :

All p38 MAPK Isoforms :

In Vitro

Paeonolide (0.1-100 µM; 24 h) does not affect viability or proliferation of MC3T3-E1 pre-osteoblasts^[1].
Paeonolide (1-30 µM; 24 h) enhances wound healing migration of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner^[1].
Paeonolide (1-30 µM; 7 days) promotes early osteoblast differentiation of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner, as measured by increased ALP staining and activity^[1].
Paeonolide (1-30 µM; 14 days) promotes late osteoblast differentiation and mineralized nodule formation of OS-induced MC3T3-E1 pre-osteoblasts in a dose-dependent manner, as measured by increased ARS staining and osteoblast-specific gene expression^[1].
Paeonolide (1-10 µM; 24 h) activates the BMP-Smad1/5/8, Wnt-β-catenin, and MAPK (ERK1/2, JNK, p38) signaling pathways in OS-induced MC3T3-E1 pre-osteoblasts, with a dominant effect on ERK1/2 phosphorylation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MC3T3-E1 pre-osteoblasts
Concentration:

0.1, 1, 10, 30, and 100 µM
Incubation Time:

24 h
Result:

Showed no cytotoxic or proliferative effects across all tested concentrations, with cell viability remaining near 100% relative to untreated controls.

Cell Migration Assay ^[1]

Cell Line:

OS-induced MC3T3-E1 pre-osteoblasts
Concentration:

1, 10, 30 µM
Incubation Time:

24 h
Result:

Promoted wound healing in a dose-dependent manner.
Showed statistically significant increases compared to OS-only controls at all concentrations.

Cell Differentiation Assay^[1]

Cell Line:

OS-induced MC3T3-E1 pre-osteoblasts
Concentration:

1, 10, 30 µM
Incubation Time:

7 days
Result:

Promoted early osteoblast differentiation in a dose-dependent manner, with increased ALP staining intensity and ALP-positive cell counts observed via microscopy.
Showed statistically significant increases compared to OS-only controls at all concentrations.

Western Blot Analysis^[1]

Cell Line:

OS-induced MC3T3-E1 pre-osteoblasts
Concentration:

1, 10 µM
Incubation Time:

24 h
Result:

Increased BMP2 protein expression and phosphorylation of Smad1/5/8 in a dose-dependent manner.
Enhanced Wnt3a protein expression, phosphorylation of GSK3β, and β-catenin protein levels in a dose-dependent manner.
Increased phosphorylation of ERK1/2, JNK, and p38 in a dose-dependent manner.
Promoted phosphorylation of ERK1/2 to a greater extent than BMP2 (100 ng/mL) treatment.

Chemical Information

CAS No. 72520-92-4
Appearance Solid
Molecular Weight 460.43
Formula C₂₀H₂₈O₁₂
Color White to off-white
SMILES

O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1CO[C@H](OC[C@H](O)[C@@H]2O)[C@@H]2O)OC(C=C(OC)C=C3)=C3C(C)=O
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (217.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1719 mL

10.8594 mL

21.7188 mL

5 mM

0.4344 mL

2.1719 mL

4.3438 mL

10 mM

0.2172 mL

1.0859 mL

2.1719 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Park KR, et al. Paeonolide as a Novel Regulator of Core-Binding Factor Subunit Alpha-1 in Bone-Forming Cells. Int J Mol Sci. 2021;22(9):4924. Published 2021 May 6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1719 mL	10.8594 mL	21.7188 mL	54.2971 mL
	5 mM	0.4344 mL	2.1719 mL	4.3438 mL	10.8594 mL
	10 mM	0.2172 mL	1.0859 mL	2.1719 mL	5.4297 mL
	15 mM	0.1448 mL	0.7240 mL	1.4479 mL	3.6198 mL
	20 mM	0.1086 mL	0.5430 mL	1.0859 mL	2.7149 mL
	25 mM	0.0869 mL	0.4344 mL	0.8688 mL	2.1719 mL
	30 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8099 mL
	40 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3574 mL
	50 mM	0.0434 mL	0.2172 mL	0.4344 mL	1.0859 mL
	60 mM	0.0362 mL	0.1810 mL	0.3620 mL	0.9050 mL
	80 mM	0.0271 mL	0.1357 mL	0.2715 mL	0.6787 mL
	100 mM	0.0217 mL	0.1086 mL	0.2172 mL	0.5430 mL

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Paeonolide

Immunology/Inflammation Compound Library

MAPK Compound Library

Stem Cell Signaling Compound Library

TGF-beta/Smad Compound Library

Wnt/Hedgehog/Notch Compound Library

Natural Product Library

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Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

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Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Pain-Related Compound Library

Extracellular Vesicles (EVs) Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

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Lactylation Compound Library

Mass Spectrometry Natural Product Library

All ERK Isoforms

All TGF-beta/Smad Isoforms

All Wnt Isoforms

All JNK Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Paeonolide Related Classifications

HY-N2156 Related Classifications