ZLY025
Based on 1 Customer Validation
ZLY025 is a potent and orally active CCNK molecular glue degrader with an DC50 of 42.7 nM. ZLY025 exhibits broad-spectrum antiproliferative activity against various tumor cells with IC50 values ranging from 0.08 to 2.45 μM. ZLY025 can induce cells apoptosis and G1 phase arrest. ZLY025 can be used for the research of cancer, such as lung cancer.
For research use only. We do not sell to patients.
- Purity: 99.40%
- CAS No.: 3070379-34-6
- Formula: C15H13N3O2S
- Molecular Weight:299.35
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Orally Active Compound Library
HY-L061
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Anti-Breast Cancer Compound Library
HY-L074
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Anti-Lung Cancer Compound Library
HY-L075
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Anti-Blood Cancer Compound Library
HY-L079
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Molecular Glue Compound Library
HY-L137
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Cell Death Library
HY-L162
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Anti-Hematopathy Compound Library
HY-L171
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
ZLY025 exhibits broad-spectrum antiproliferative activity against various tumor cell lines including lung cancer, breast cancer, liver cancer, and leukemia, with IC50 values ranging from 0.08 to 2.45 μM[1].
ZLY025 (125-500 nM; 24 h) induces A549 cells apoptosis and G1 phase arrest[1].
ZLY025 (30-1000 nM; 8-24 h) reduces expression of CCNK, CDK12, p-CTD, and BRCA1 in A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:125, 250, 500 nM
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Incubation Time:24 h
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Result:Increased apoptosis rate.
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Cell Line:A549 cells
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Concentration:125, 250, 500 nM
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Incubation Time:24 h
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Result:Induced G1 phase arrest.
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Cell Line:A549 cells
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Concentration:30, 100, 300, 1000 nM
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Incubation Time:8, 16, 24 h
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Result:Decreased expression of CCNK, CDK12, p-CTD, and BRCA1.
| Species | Dose | Route | Note | Tmax | Cmax | T1/2 | AUC0-t | CL |
|---|---|---|---|---|---|---|---|---|
| Rat | 3 mg/kg | p.o. | 文献审核 | 0.6 h | 180.18 ng/mL | 6.82 h | 1184.94 ng·h/mL | 2.6 L/h/kg |
ZLY025 (200 mg/kg, i.g., once daily for 14 days) shows extremely low subacute toxicity in ICR mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude (female, subcutaneous injection of A549 human non-small cell lung cancer (NSCLC) cells)[1]
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Dosage:10, 20, 40 mg/kg
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Administration:Oral gavage; once daily for 17 days
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Result:Reduced tumor weight and volume.
Did not affect mouse body weight or cause toxicity to major organs.
Chemical Information
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CAS No. 3070379-34-6
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Appearance Solid
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Molecular Weight 299.35
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Formula C15H13N3O2S
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Color White to off-white
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SMILES
CC1=CN=C(NC(CC2=CC=C(C=C2)C3=NC=CO3)=O)S1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 5 mg/mL (16.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (1.67 mM); Clear solution
This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3406 mL | 16.7029 mL | 33.4057 mL | 83.5143 mL |
| 5 mM | 0.6681 mL | 3.3406 mL | 6.6811 mL | 16.7029 mL | |
| 10 mM | 0.3341 mL | 1.6703 mL | 3.3406 mL | 8.3514 mL | |
| 15 mM | 0.2227 mL | 1.1135 mL | 2.2270 mL | 5.5676 mL |
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