Art. -Nr.: HY-B1839

Fluazinam

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Fluazinam is an orally active dinitroaniline fungicide. Fluazinam induces phosphorylation of JNK, activates p38 pathway, decreases Bcl-2, activates caspase-3, decreases complex I activity, increases Autophagy and Apoptosis. Fluazinam has strong antifungal activity against F. fujikuroi and B. maydis. Fluazinam has a negative impact on Brachydanio rerio and worker bees.

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Reinheit: 99.51%
CAS. Nr.: 79622-59-6
Formel: C₁₃H₄Cl₂F₆N₄O₄
Molecular Weight:465.09

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Fluazinam Chemical Structure

CAS. Nr.: 79622-59-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Fungal^[1]

Alle p38 MAPK Isoform-spezifische Produkte anzeigen :

Alle Caspase Isoform-spezifische Produkte anzeigen :

Alle JNK Isoform-spezifische Produkte anzeigen :

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

In Vitro

Fluazinam (0-5 μM; 24 h) induces SH-SY5Y cell death and nuclear damage^[1].
Fluazinam (10-20 μg/mL) has great control efficiency against B. maydis on detached corn leaves^[2].
Fluazinam has strong antifungal activity against F. fujikuroi, with EC₅₀ values ranging from 0.0621 to 0.5446 μg/mL for mycelial growth inhibition of 103 F. fujikuroi isolates^[3].
Fluazinam (0.01-0.5 μM) impairs oxidative phosphorylation and induces hyper/hypo-activity in a dose specific manner in zebrafish larvae^[4].
Fluazinam (0.03-0.40 mg/L; 24-96 h) has acute toxicity to Brachydanio rerio^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

SH-SY5Y
Concentration:

1.25, 2.5, 5 μM
Incubation Time:

10 min, 30 min, 1 h, 2 h, 4 h, 8 h, 16 h, 24 h
Result:

Induced phosphorylation of JNK.
Activated the p38 pathway.
Induced a decrease in the level of Bcl-2 protein.
Activated caspase-3 in a dose-dependent manner.
Decreased complex I activity.

In Vivo

Fluazinam (0.5-2.5 mg a.i. mL^-1; oral exposure; 24 h) reduces food consumption, damages the midgut epithelium, increases autophagy and apoptosis in the midgut, induces oxidative stress, and alters the walking behavior in Partamona helleri worker bees^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 79622-59-6
Appearance Solid
Molecular Weight 465.09
Formel C₁₃H₄Cl₂F₆N₄O₄
Color Light yellow to yellow
SMILES

FC(C1=CN=C(NC2=C([N+]([O-])=O)C=C(C(F)(F)F)C(Cl)=C2[N+]([O-])=O)C(Cl)=C1)(F)F
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (268.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1501 mL

10.7506 mL

21.5012 mL

5 mM

0.4300 mL

2.1501 mL

4.3002 mL

10 mM

0.2150 mL

1.0751 mL

2.1501 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (923 KB)
- English - EN (923 KB)
- Français - FR (923 KB)
- Deutsch - DE (923 KB)
- Norwegian - NO (923 KB)
- Español - ES (923 KB)
- Swedish - SV (923 KB)
- Italian - IT (923 KB)
- Korean - KR (923 KB)
- Portuguese - PT (923 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lee JE, et al. Fluazinam targets mitochondrial complex I to induce reactive oxygen species-dependent cytotoxicity in SH-SY5Y cells. Neurochem Int. 2012 Jun;60(8):773-81. [Content Brief]

[2]. Chen YL, et al. Activity of the dinitroaniline fungicide fluazinam against Bipolaris maydis. Pestic Biochem Physiol. 2018 Jun;148:8-15. [Content Brief]

[3]. Qu XP, et al. Effects of the dinitroaniline fungicide fluazinam on Fusarium fujikuroi and rice. Pestic Biochem Physiol. 2018 Nov;152:98-105. [Content Brief]

[4]. Wang XH, et al. Fluazinam impairs oxidative phosphorylation and induces hyper/hypo-activity in a dose specific manner in zebrafish larvae. Chemosphere. 2018 Nov;210:633-644. [Content Brief]

[5]. Wu W, et al. Acute toxicity of fluazinam to aquatic organisms and its bioaccumulation in Brachydanio rerio. Environ Sci Pollut Res Int. 2020 Oct;27(28):35000-35007. [Content Brief]

[6]. Gomes DS, et al. Acute exposure to fungicide fluazinam induces cell death in the midgut, oxidative stress and alters behavior of the stingless bee Partamona helleri (Hymenoptera: Apidae). Ecotoxicol Environ Saf. 2024 Sep 1;282:116677. [Content Brief]

[7]. Hou YP, et al. Impact of fluazinam on morphological and physiological characteristics of Sclerotinia sclerotiorum. Pestic Biochem Physiol. 2019 Mar;155:81-89. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1501 mL	10.7506 mL	21.5012 mL	53.7530 mL
	5 mM	0.4300 mL	2.1501 mL	4.3002 mL	10.7506 mL
	10 mM	0.2150 mL	1.0751 mL	2.1501 mL	5.3753 mL
	15 mM	0.1433 mL	0.7167 mL	1.4334 mL	3.5835 mL
	20 mM	0.1075 mL	0.5375 mL	1.0751 mL	2.6877 mL
	25 mM	0.0860 mL	0.4300 mL	0.8600 mL	2.1501 mL
	30 mM	0.0717 mL	0.3584 mL	0.7167 mL	1.7918 mL
	40 mM	0.0538 mL	0.2688 mL	0.5375 mL	1.3438 mL
	50 mM	0.0430 mL	0.2150 mL	0.4300 mL	1.0751 mL
	60 mM	0.0358 mL	0.1792 mL	0.3584 mL	0.8959 mL
	80 mM	0.0269 mL	0.1344 mL	0.2688 mL	0.6719 mL
	100 mM	0.0215 mL	0.1075 mL	0.2150 mL	0.5375 mL

Keywords

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Fluazinam

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

MAPK Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Oxygen Sensing Compound Library

Antifungal Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Anti-Lung Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Mitochondria-Targeted Compound Library

Transcription Factor-Targeted Library

Osteogenesis Compound Library

Pain-Related Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Pesticide Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Environmental Pollutant Compound Library

Lactylation Compound Library

Alle p38 MAPK Isoform-spezifische Produkte anzeigen

Alle Caspase Isoform-spezifische Produkte anzeigen

Alle JNK Isoform-spezifische Produkte anzeigen

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Fluazinam Related Classifications

HY-B1839 Related Classifications