1. Stem Cell/Wnt
  2. Hedgehog Smo
  3. Jervine

Jervine  (Synonyms: 11-Ketocyclopamine)

Cat. No.: HY-N0836 Purity: 98.03%
COA Handling Instructions

Jervine (11-Ketocyclopamine) is a potent Hedgehog (Hh) inhibitor with an IC50 of 500-700 nM. Jervine is a natural teratogenic sterodial alkaloid from rhizomes of Veratrum nigrum. Jervine has anti-inflammatory and antioxidant properties.

For research use only. We do not sell to patients.

Jervine Chemical Structure

Jervine Chemical Structure

CAS No. : 469-59-0

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5 mg USD 81 In-stock
10 mg USD 150 In-stock
50 mg USD 685 In-stock
100 mg USD 1200 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

Jervine (11-Ketocyclopamine) is a potent Hedgehog (Hh) inhibitor with an IC50 of 500-700 nM[1]. Jervine is a natural teratogenic sterodial alkaloid from rhizomes of Veratrum nigrum. Jervine has anti-inflammatory and antioxidant properties[2].

IC50 & Target

IC50: 500-700 nM (Hedgehog)[1]

In Vitro

Jervine (40 μM; 6, 12 and 24 hours) inhibits Akt phosphorylation[3].
Jervine (40 μM; 2 hours) inhibits NF-jB activation decreased COX-2 overexpression and induced apoptosis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HEL and TF1a cells
Concentration: 40 μM
Incubation Time: 6, 12 and 24 hours
Result: Inhibited Akt phosphorylation.
In Vivo

Jervine (orally; 50-400 mg/kg) exerts 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague Dawley rats (180-200  g)[2]
Dosage: 50, 100, 200 and 400  mg/kg
Administration: Orally
Result: Exerted 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema.
Molecular Weight

425.60

Formula

C27H39NO3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@]2([H])[C@]3(C)C(C[C@@H](O)CC3)=CC[C@@]2([H])[C@]4([H])CC[C@]5(O[C@@]6([H])[C@@]([C@H]5C)([H])NC[C@@H](C)C6)C(C)=C41

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 1 mg/mL (2.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3496 mL 11.7481 mL 23.4962 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.03%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3496 mL 11.7481 mL 23.4962 mL 58.7406 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Jervine
Cat. No.:
HY-N0836
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