2. Metabolic Enzyme/Protease
  3. MMP
  4. NSC 405020

NSC 405020 is a specific MMP14 inhibitor. NSC 405020 can directly interact with the hemopexin domain of MMP14. NSC 405020 reduces the expression of full length and active cleaved Notch3 (NICD3). NSC 405020 can be used to research vestibular schwannoma, hemostasis and thrombosis.

For research use only. We do not sell to patients.

CAS No. : 7497-07-6

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Based on 3 publication(s) in Google Scholar

NSC 405020 Related Antibodies

Top Publications Citing Use of Products
In Vivo Efficacy Study
Cell Proliferation/Viability Assay
WB

    NSC 405020 purchased from MedChemExpress. Usage Cited in: Exp Hematol Oncol. 2025 Mar 22;14(1):43.  [Abstract]

    A-D. AML cell lines, Kasumi-1 and Molm-13, were co-cultured with MSCs and treated for 48 hours with the inhibitor (NSC 405020; 10, 20 μM), Ara-C (0.5, 1 μM), or their combination. Apoptosis rates were measured by flow cytometry, andthe combination index (CI) was calculated using CompuSyn software, revealing synergistic effects (CI < 1) between the inhibitor and Ara-C.

    NSC 405020 purchased from MedChemExpress. Usage Cited in: Exp Hematol Oncol. 2025 Mar 22;14(1):43.  [Abstract]

    AML cell lines, Kasumi-1 and Molm-13, were co-cultured with MSCs and treated for 48 hours with the inhibitor (NSC 405020; 20 μM), Ara-C (1 μM), or their combination. Western blot analysis of STAT3, P-STAT3, STAT5, and P-STAT5 protein expression in Kasumi-1 and Molm-13 cells co-cultured with MSCs.

    NSC 405020 purchased from MedChemExpress. Usage Cited in: Exp Hematol Oncol. 2025 Mar 22;14(1):43.  [Abstract]

    MMP14 inhibitor (NSC 405020) combined with cytarabine group showed highest survival rate and the proportion of bone marrow and splenic leukemia cells in mice treated with the combination of MMP14 inhibitor and cytarabine was significantly lower than in the control and single-agent groups.

    NSC 405020 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2018 Nov 20;25(8):2163-2176.  [Abstract]

    NSC-405020 (10 mg/kg; ip; every 3 days during the 2 weeks) in fib-mdx mice showed a significant improvement in the muscle, including reduction in necrosis (60%), reduction in fibrosis (11%).

    NSC 405020 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2018 Nov 20;25(8):2163-2176.  [Abstract]

    NSC-405020 (10 mg/kg; ip; every 3 days during the 2 weeks) in fib-mdx mice showed a myofiber CSA distribution shift to larger myofibers.

    View All MMP Isoform Specific Products:

    View All Isoforms
    MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    NSC 405020 is a specific MMP14 inhibitor. NSC 405020 can directly interact with the hemopexin domain of MMP14. NSC 405020 reduces the expression of full length and active cleaved Notch3 (NICD3). NSC 405020 can be used to research vestibular schwannoma, hemostasis and thrombosis[1][2][3].

    IC50 & Target

    MMP14[1]
    In Vitro

    NSC 405020 (100 and 1000 μM; 0-30 min) inhibits MMP-14 levels by over 85% with a dose-dependent manner in the plasma sample from vestibular schwannoma group[1].
    ? NSC 405020 (50 μM) reduces expression of the full length and active cleaved Notch3 (NICD3) by 42% in the WM852 cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    NSC 405020 Related Antibodies
    Molecular Weight

    260.16

    Formula

    C12H15Cl2NO
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NC(C)CCC)C1=CC=C(Cl)C(Cl)=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 260 mg/mL (999.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8438 mL 19.2189 mL 38.4379 mL
    5 mM 0.7688 mL 3.8438 mL 7.6876 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (9.61 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.8438 mL 19.2189 mL 38.4379 mL 96.0947 mL
    5 mM 0.7688 mL 3.8438 mL 7.6876 mL 19.2189 mL
    10 mM 0.3844 mL 1.9219 mL 3.8438 mL 9.6095 mL
    15 mM 0.2563 mL 1.2813 mL 2.5625 mL 6.4063 mL
    20 mM 0.1922 mL 0.9609 mL 1.9219 mL 4.8047 mL
    25 mM 0.1538 mL 0.7688 mL 1.5375 mL 3.8438 mL
    30 mM 0.1281 mL 0.6406 mL 1.2813 mL 3.2032 mL
    40 mM 0.0961 mL 0.4805 mL 0.9609 mL 2.4024 mL
    50 mM 0.0769 mL 0.3844 mL 0.7688 mL 1.9219 mL
    60 mM 0.0641 mL 0.3203 mL 0.6406 mL 1.6016 mL
    80 mM 0.0480 mL 0.2402 mL 0.4805 mL 1.2012 mL
    100 mM 0.0384 mL 0.1922 mL 0.3844 mL 0.9609 mL
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    NSC 405020 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    NSC 4050207497-07-6NSC405020NSC-405020MMPMatrix metalloproteinasesvestibular schwannomahemostasisthrombosisNotch3NICD3WM852 cellsInhibitorinhibitorinhibit

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