Cat. No.: HY-B0926A

Sodium diatrizoate

Name: Sodium diatrizoate
Brand: MedChemExpress
SKU: HY-B0926A
Price: 40.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Diatrizoic acid sodium salt; Sodium amidotrizoate)

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Sodium diatrizoate (Diatrizoic acid sodium salt) is an iodinated contrast agent. Sodium diatrizoate induces intracellular calcium homeostasis dysregulation, activates calpain and caspase-12, and activates the executor caspase-3, inducing cell apoptosis. Sodium diatrizoate induces mitophagy, oxidative stress, and TNFα secretion/inflammatory responses, while inhibiting mitochondrial respiratory function. Sodium diatrizoate can be used for research on contrast-induced acute kidney injury (ci-aki).

For research use only. We do not sell to patients.

Purity : 99.55%
CAS No.: 737-31-5
Formula: C₁₁H₈I₃N₂NaO₄
Molecular Weight:635.90

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Sodium diatrizoate Chemical Structure

CAS No.: 737-31-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

Caspase-12

calpain

Caspase-3

TNFα

All Caspase Isoforms :

All TNF Receptor Isoforms :

In Vitro

ApoptosisAutophagyLysosomal MetabolismCarbohydrate MetabolismCytokines & ChemokinesOxidative StressMitochondrial FunctionMitochondrial Oxidative PhosphorylationProtein/Amino Acid MetabolismOthers

Apoptosis

Diatrizoic acid (18-30 mg I/mL; 24 h) sodium salt induces cytochrome c release from HK-2 mitochondria at 24 h^[1].
Diatrizoic acid (15-30 mg I/mL; 24 h) sodium salt activates caspase 3 and caspase 12 in HK-2 cells starting from 28 mg I/mL at 24 h, but does not activate caspase 4^[1].

Autophagy

Lysosomal Metabolism

Diatrizoic acid (15-30 mg I/mL; 8-24 h) sodium salt increases the LC3BII/LC3BI ratio in HK-2 cells at 8 h, suggesting increased mitophagy^[1].
Diatrizoic acid (15-30 mg I/mL; 8-24 h) sodium salt increases 4HNE protein adduct formation in HK-2 cells starting from 18 mg I/mL at 24 h^[1].
Diatrizoic acid (30 mg I/mL; 24 h) sodium salt induces LC3B conversion in HK-2 cells, which is prevented by intracellular calcium chelation and partially attenuated by calpain inhibition^[1].
Diatrizoic acid (30 mg I/mL; 24 h) sodium salt induces caspase 12 activation in HK-2 cells, and this activation is attenuated by intracellular calcium chelation and calpain inhibition^[1].

Carbohydrate Metabolism

Diatrizoic acid (15-30 mg I/mL; 8-24 h) sodium salt reduces HK-2 glycolysis and glycolytic capacity starting from 28 mg I/mL at 8 h and from 23 mg I/mL at 24 h^[1].

Cytokines & Chemokines

Oxidative Stress

Diatrizoic acid (15-30 mg I/mL; 24 h) sodium salt decreases TNFα in HK-2 lysates and increases TNFα in the culture medium at high concentrations at 24 h, without altering NOX4 expression^[1].

Mitochondrial Function

Diatrizoic acid (2-30 mg I/mL; 2-24 h) sodium salt reduces HK-2 mitochondrial viability as early as 2 h at 15 mg I/mL^[1].
Diatrizoic acid (15-30 mg I/mL; 8-24 h) sodium salt impairs HK-2 mitochondrial respiration at 8 h, with maximal respiratory capacity and spare respiratory capacity decreasing from 15 mg I/mL, and a broader range of respiratory defects at 24 h^[1].
Diatrizoic acid (15-30 mg I/mL; 2-24 h) sodium salt induces mitochondrial toxicity in HK-2 cells, which is prevented by intracellular calcium chelation and partially attenuated by extracellular calcium chelation or IP3 receptor antagonism^[1].

Mitochondrial Function

Mitochondrial Oxidative Phosphorylation

Carbohydrate Metabolism

Diatrizoic acid (15-30 mg I/mL; 24 h) sodium salt decreases total ATP and mitochondrial ATP production in HK-2 cells starting from 18 mg I/mL at 24 h, without decreasing glycolytic ATP production^[1].

Oxidative Stress

Diatrizoic acid (15-30 mg I/mL; 8-24 h) sodium salt increases protein carbonylation in HK-2 cells from 18 mg I/mL at 24 h^[1].
Diatrizoic acid (15-30 mg I/mL; 24 h) sodium salt decreases total SOD and Cu/ZnSOD activity in HK-2 cells at 24 h, while MnSOD activity and expression remain unchanged^[1].
Diatrizoic acid (30 mg I/mL; 24 h) sodium salt induces 4HNE adduct formation in HK-2 cells, and this formation depends on intracellular calcium and calpain activity^[1].

Protein/Amino Acid Metabolism

Diatrizoic acid (30 mg I/mL; 24 h) sodium salt activates calpain in HK-2 cells^[1].

Others

Diatrizoic acid (2-30 mg I/mL; 2-24 h) sodium salt reduces HK-2 cell viability only after 24 h of exposure, with an onset concentration of 23 mg I/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HK-2 cells
Concentration:

2, 5, 10, 15, 18, 23, 28, and 30 mg I/mL
Incubation Time:

2, 8, and 24 h
Result:

Concentration-dependent decreases were evident at 8 h and 24 h, and a time-dependent decrease was evident across 2, 8, and 24 h.\nNo decrease in cell viability was seen at 2 h or 8 h.
At 24 h, viability decreased at 23 mg I/mL and higher, with additional decline at 28 and 30 mg I/mL.

Western Blot Analysis^[2]

Cell Line:

HK-2 cells
Concentration:

15, 18, 23, 28, and 30 mg I/mL
Incubation Time:

8 and 24 h
Result:

LC3BI decreased at 8 h at 18-30 mg I/mL and at 24 h at 15-30 mg I/mL.
LC3BII trended upward at 8 h but was not different; LC3BII increased at 24 h.
The LC3BII/LC3BI ratio increased at both 8 h and 24 h.\n4HNE adduct formation was unchanged at 8 h.
At 24 h, 4HNE adduct formation increased at 18-30 mg I/mL.

Western Blot Analysis^[2]

Cell Line:

HK-2 cells
Concentration:

18 and 30 mg I/mL
Incubation Time:

24 h
Result:

Cytosolic cytochrome c increased at 18 and 30 mg I/mL.
Mitochondrial cytochrome c decreased at 30 mg I/mL.

Western Blot Analysis^[2]

Cell Line:

HK-2 cells
Concentration:

15, 18, 23, 28, and 30 mg I/mL
Incubation Time:

24 h
Result:

Cleaved caspase 3 and caspase 12 expression increased at 28 and 30 mg I/mL.
Caspase 4 expression was unchanged.

Related Protocols :

Chemical Information

CAS No. 737-31-5
Appearance Solid
Molecular Weight 635.90
Formula C₁₁H₈I₃N₂NaO₄
Color White to off-white
SMILES

O=C(O[Na])C1=C(I)C(NC(C)=O)=C(I)C(NC(C)=O)=C1I
Synonyms

Diatrizoic acid sodium salt; Sodium amidotrizoate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (157.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (78.63 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5726 mL

7.8629 mL

15.7257 mL

5 mM

0.3145 mL

1.5726 mL

3.1451 mL

10 mM

0.1573 mL

0.7863 mL

1.5726 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 33.33 mg/mL (52.41 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ward DB, et al. Radiocontrast Agent Diatrizoic Acid Induces Mitophagy and Oxidative Stress via Calcium Dysregulation. International journal of molecular sciences. 2019 Aug 21;20(17):4074. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	1.5726 mL	7.8629 mL	15.7257 mL	39.3144 mL
	5 mM	0.3145 mL	1.5726 mL	3.1451 mL	7.8629 mL
	10 mM	0.1573 mL	0.7863 mL	1.5726 mL	3.9314 mL
	15 mM	0.1048 mL	0.5242 mL	1.0484 mL	2.6210 mL
	20 mM	0.0786 mL	0.3931 mL	0.7863 mL	1.9657 mL
	25 mM	0.0629 mL	0.3145 mL	0.6290 mL	1.5726 mL
	30 mM	0.0524 mL	0.2621 mL	0.5242 mL	1.3105 mL
	40 mM	0.0393 mL	0.1966 mL	0.3931 mL	0.9829 mL
	50 mM	0.0315 mL	0.1573 mL	0.3145 mL	0.7863 mL
	60 mM	0.0262 mL	0.1310 mL	0.2621 mL	0.6552 mL
DMSO	80 mM	0.0197 mL	0.0983 mL	0.1966 mL	0.4914 mL
DMSO	100 mM	0.0157 mL	0.0786 mL	0.1573 mL	0.3931 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sodium diatrizoate

All Caspase Isoforms

All TNF Receptor Isoforms