Cat. No.: HY-B0926

Diatrizoic acid

Name: Diatrizoic acid
Brand: MedChemExpress
SKU: HY-B0926
Price: 40.00 USD
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(Synonyms: Diatrizoate; Amidotrizoic acid)

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Diatrizoic acid (Diatrizoate) is an iodinated contrast agent. Diatrizoic acid induces intracellular calcium homeostasis dysregulation, activates calpain and caspase-12, and activates the executor caspase-3, inducing cell apoptosis. Diatrizoic acid induces mitophagy, oxidative stress, and TNFα secretion/inflammatory responses, while inhibiting mitochondrial respiratory function. Diatrizoic acid can be used for research on contrast-induced acute kidney injury (ci-aki).

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Pureté : 99.73%
CAS No.: 117-96-4
Formule: C₁₁H₉I₃N₂O₄
Masse moléculaire:613.91

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Diatrizoic acid Chemical Structure

CAS No.: 117-96-4

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- Essai clinique
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

^[1]

Caspase-12

calpain

Caspase-3

TNFα

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In Vitro

ApoptosisAutophagyLysosomal MetabolismCarbohydrate MetabolismCytokines & ChemokinesOxidative StressMitochondrial FunctionMitochondrial Oxidative PhosphorylationProtein/Amino Acid MetabolismOthers

Apoptosis

Diatrizoic acid (18-30 mg I/mL; 24 h) induces cytochrome c release from HK-2 mitochondria at 24 h^[1].
Diatrizoic acid (15-30 mg I/mL; 24 h) activates caspase 3 and caspase 12 in HK-2 cells starting from 28 mg I/mL at 24 h, but does not activate caspase 4^[1].

Autophagy

Lysosomal Metabolism

Diatrizoic acid (15-30 mg I/mL; 8-24 h) increases the LC3BII/LC3BI ratio in HK-2 cells at 8 h, suggesting increased mitophagy^[1].
Diatrizoic acid (15-30 mg I/mL; 8-24 h) increases 4HNE protein adduct formation in HK-2 cells starting from 18 mg I/mL at 24 h^[1].
Diatrizoic acid (30 mg I/mL; 24 h) induces LC3B conversion in HK-2 cells, which is prevented by intracellular calcium chelation and partially attenuated by calpain inhibition^[1].
Diatrizoic acid (30 mg I/mL; 24 h) induces caspase 12 activation in HK-2 cells, and this activation is attenuated by intracellular calcium chelation and calpain inhibition^[1].

Carbohydrate Metabolism

Diatrizoic acid (15-30 mg I/mL; 8-24 h) reduces HK-2 glycolysis and glycolytic capacity starting from 28 mg I/mL at 8 h and from 23 mg I/mL at 24 h^[1].

Cytokines & Chemokines

Oxidative Stress

Diatrizoic acid (15-30 mg I/mL; 24 h) decreases TNFα in HK-2 lysates and increases TNFα in the culture medium at high concentrations at 24 h, without altering NOX4 expression^[1].

Mitochondrial Function

Diatrizoic acid (2-30 mg I/mL; 2-24 h) reduces HK-2 mitochondrial viability as early as 2 h at 15 mg I/mL^[1].
Diatrizoic acid (15-30 mg I/mL; 8-24 h) impairs HK-2 mitochondrial respiration at 8 h, with maximal respiratory capacity and spare respiratory capacity decreasing from 15 mg I/mL, and a broader range of respiratory defects at 24 h^[1].
Diatrizoic acid (15-30 mg I/mL; 2-24 h) induces mitochondrial toxicity in HK-2 cells, which is prevented by intracellular calcium chelation and partially attenuated by extracellular calcium chelation or IP3 receptor antagonism^[1].

Mitochondrial Function

Mitochondrial Oxidative Phosphorylation

Carbohydrate Metabolism

Diatrizoic acid (15-30 mg I/mL; 24 h) decreases total ATP and mitochondrial ATP production in HK-2 cells starting from 18 mg I/mL at 24 h, without decreasing glycolytic ATP production^[1].

Oxidative Stress

Diatrizoic acid (15-30 mg I/mL; 8-24 h) increases protein carbonylation in HK-2 cells from 18 mg I/mL at 24 h^[1].
Diatrizoic acid (15-30 mg I/mL; 24 h) decreases total SOD and Cu/ZnSOD activity in HK-2 cells at 24 h, while MnSOD activity and expression remain unchanged^[1].
Diatrizoic acid (30 mg I/mL; 24 h) induces 4HNE adduct formation in HK-2 cells, and this formation depends on intracellular calcium and calpain activity^[1].

Protein/Amino Acid Metabolism

Diatrizoic acid (30 mg I/mL; 24 h) activates calpain in HK-2 cells^[1].

Others

Diatrizoic acid (2-30 mg I/mL; 2-24 h) reduces HK-2 cell viability only after 24 h of exposure, with an onset concentration of 23 mg I/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HK-2 cells
Concentration:

2, 5, 10, 15, 18, 23, 28, and 30 mg I/mL
Incubation Time:

2, 8, and 24 h
Result:

Concentration-dependent decreases were evident at 8 h and 24 h, and a time-dependent decrease was evident across 2, 8, and 24 h.\nNo decrease in cell viability was seen at 2 h or 8 h.
At 24 h, viability decreased at 23 mg I/mL and higher, with additional decline at 28 and 30 mg I/mL.

Western Blot Analysis^[2]

Cell Line:

HK-2 cells
Concentration:

15, 18, 23, 28, and 30 mg I/mL
Incubation Time:

8 and 24 h
Result:

LC3BI decreased at 8 h at 18-30 mg I/mL and at 24 h at 15-30 mg I/mL.
LC3BII trended upward at 8 h but was not different; LC3BII increased at 24 h.
The LC3BII/LC3BI ratio increased at both 8 h and 24 h.\n4HNE adduct formation was unchanged at 8 h.
At 24 h, 4HNE adduct formation increased at 18-30 mg I/mL.

Western Blot Analysis^[2]

Cell Line:

HK-2 cells
Concentration:

18 and 30 mg I/mL
Incubation Time:

24 h
Result:

Cytosolic cytochrome c increased at 18 and 30 mg I/mL.
Mitochondrial cytochrome c decreased at 30 mg I/mL.

Western Blot Analysis^[2]

Cell Line:

HK-2 cells
Concentration:

15, 18, 23, 28, and 30 mg I/mL
Incubation Time:

24 h
Result:

Cleaved caspase 3 and caspase 12 expression increased at 28 and 30 mg I/mL.
Caspase 4 expression was unchanged.

Related Protocols :

Essai clinique

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Chemical Information

CAS No. 117-96-4
Appearance Solid
Masse moléculaire 613.91
Formule C₁₁H₉I₃N₂O₄
Color White to off-white
SMILES

O=C(O)C1=C(I)C(NC(C)=O)=C(I)C(NC(C)=O)=C1I
Synonyms

Diatrizoate; Amidotrizoic acid
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (162.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6289 mL

8.1445 mL

16.2890 mL

5 mM

0.3258 mL

1.6289 mL

3.2578 mL

10 mM

0.1629 mL

0.8145 mL

1.6289 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (282 KB)
SDS (393 KB)
- English - EN (393 KB)
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- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Ward DB, et al. Radiocontrast Agent Diatrizoic Acid Induces Mitophagy and Oxidative Stress via Calcium Dysregulation. International journal of molecular sciences. 2019 Aug 21;20(17):4074. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6289 mL	8.1445 mL	16.2890 mL	40.7226 mL
	5 mM	0.3258 mL	1.6289 mL	3.2578 mL	8.1445 mL
	10 mM	0.1629 mL	0.8145 mL	1.6289 mL	4.0723 mL
	15 mM	0.1086 mL	0.5430 mL	1.0859 mL	2.7148 mL
	20 mM	0.0814 mL	0.4072 mL	0.8145 mL	2.0361 mL
	25 mM	0.0652 mL	0.3258 mL	0.6516 mL	1.6289 mL
	30 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3574 mL
	40 mM	0.0407 mL	0.2036 mL	0.4072 mL	1.0181 mL
	50 mM	0.0326 mL	0.1629 mL	0.3258 mL	0.8145 mL
	60 mM	0.0271 mL	0.1357 mL	0.2715 mL	0.6787 mL
	80 mM	0.0204 mL	0.1018 mL	0.2036 mL	0.5090 mL
	100 mM	0.0163 mL	0.0814 mL	0.1629 mL	0.4072 mL

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Diatrizoic acid

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