NSC647889
Based on 1 Customer Validation
NSC647889 is an apoptosis and autophagy inducer. NSC647889 induces apoptosis, inhibits mTOR pathway and abrogates DNA synthesis. NSC647889 triggers LC3-positive vesicle formation, modulates AKT and 4EBP1 phosphorylation and shows heightened caspase-3 activation in multicellular spheroids. NSC647889 can be used for the research of solid cancer tumour, head-neck carcinoma, and colorectal cancer.
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- Pureté: 99.28%
- CAS No.: 1033931-92-8
- Formule: C33H48N4OS
- Masse moléculaire:548.83
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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Caspase-3 |
NSC-647889 (2-20 μM; 4-10 h) potently induces apoptosis in ~80% of HCT116 colon carcinoma multicellular spheroid cells (excluding hypoxic core cells) at 10 μM, reduces spheroid cell clonogenicity to ~1% at 20 μM, and shows a ~2.5-fold lower viability reduction potency in spheroids compared to monolayer cultures[2].
NSC-647889 (10 μM; 1 h) increases Ca2+/calmodulin-dependent calcineurin activity in HCT116 colon carcinoma cells[2].
NSC-647889 (1.25-10 μM; 1 h) increases intracellular calcium levels in HCT116 colon carcinoma cells in a dose-dependent manner, with an 8-fold increase over control at 7.5 μM after 1 h incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 colon carcinoma multicellular spheroids (MCS) and monolayer cultures
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Concentration:2 μM; 5 μM; 10 μM; 20 μM
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Incubation Time:4 h; 6 h; 8 h; 10 h
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Result:Induced apoptosis in ~80% of HCT116 spheroid cells, with apoptosis restricted to cells 0-100 µm from the spheroid surface.
Reduced clonogenicity of cells dispersed from treated spheroids to <5% of solvent-treated controls at 10 μM, and to ~1% at 20 μM.
Showed a ~2.5-fold difference in viability reduction potency between monolayer and spheroid cultures, with spheroids showing lower sensitivity.
Increased apoptosis induction (ccK18 levels) with higher concentrations in both culture systems, with monolayer cultures showing stronger apoptosis at lower doses.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID mice[2]
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Dosage:5 mg/kg
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Administration:s.c.; single dose
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Result:Increased plasma caspase-cleaved K18 (ccK18) levels significantly compared to vehicle control.
Increased total K18 levels significantly compared to vehicle control.
Chemical Information
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CAS No. 1033931-92-8
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Appearance Solid
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Masse moléculaire 548.83
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Formule C33H48N4OS
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](CCC1C[C@H](CC2)NC(NC(C4=NC=CS4)CC5=CC=CC=C5)=O)([H])[C@@]6([H])[C@](CC3)([C@H](CC6)N(C)C)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 2.2 mg/mL (4.01 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8221 mL | 9.1103 mL | 18.2206 mL | 45.5514 mL |
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