Difopein
Difopein is a 14-3-3 protein inhibitor. Difopein acts as an apoptosis inducer, regulates apoptosis-related proteins, downregulates Bcl-2, upregulates Bax, activates caspase-9 and caspase-3, and induces nuclear fragmentation, membrane-enclosed apoptotic bodies and DNA ladder formation. Difopein serves as a tumor growth inhibitor, which inhibits the proliferation of glioma cells and induces their apoptosis in in vivo nude mouse models. Difopein is applicable to glioma-related research.
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- CAS No.: 396834-58-5
- Formule: C273H424N76O89S6
- Masse moléculaire:6387.13
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Activité biologique
Description
IC50 & Target
[1]|
Caspase-9 |
Caspase-3 |
Bax |
In Vitro
Difopein (24-48 h) induces time-dependent apoptosis in human U251 and U87 glioma cells, causes time-dependent increases in both early and late apoptosis, and triggers apoptotic DNA fragmentation in U251 cells[1].
Difopein (24-48 h) alters the cell cycle distribution of U251 cells, causing time-dependent cell arrest at the G1 phase, while the proportions of cells in the S and G2 phases decrease; at 24 h and 48 h post-transfection, the proportions of G1-phase cells reach 73.9% and 84.8%, respectively[1].
Difopein (24-48 h) downregulates the anti-apoptotic protein Bcl-2 and upregulates the pro-apoptotic protein Bax at both mRNA and protein levels, and activates caspase-9 and caspase-3 in human U251 glioma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- Nuclear DNA counterstaining and nuclear morphology staining
- Patient-Derived Xenograft (PDX)
- Apoptosis Solutions
- Patient-Derived Orthotopic Xenograft (PDOX)
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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CAS No. 396834-58-5
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Masse moléculaire 6387.13
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Formule C273H424N76O89S6
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Sequence
Ser-Ala-Asp-Gly-Ala-Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala-Asn-Met-Cys-Leu-Pro-Gly-Ala-Ala-Gly-Leu-Asp-Ser-Ala-Asp-Gly-Ala-Pro-His-Cys-Val-Pro-Arg-Asp-Leu-Ser-Trp-Leu-Asp-Leu-Glu-Ala-Asn-Met-Cys-Leu-Pro-Gly-Ala-Ala-Gly-Leu-Glu
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Sequence Shortening
SADGAPHCVPRDLSWLDLEANMCLPGAAGLDSADGAPHCVPRDLSWLDLEANMCLPGAAGLE
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Difopein Related Classifications
HY-P1380 Related Classifications
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