Art. -Nr.: HY-17565A

Bleomycin hydrochloride

212 Cited Publications

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Bleomycin hydrochloride is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin hydrochloride is an antitumor antibiotic.

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Reinheit: 99.52%
CAS. Nr.: 67763-87-5

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Bleomycin hydrochloride Chemical Structure

CAS. Nr.: 67763-87-5

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Use of MCE Products

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
- Induced Disease Models
Chemical Information
Publications (212)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
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Calculators
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Biologische Aktivität

Beschreibung

Bleomycin hydrochloride is a DNA synthesis inhibitor. Bleomycin hydrochloride is a DNA damaging agent. Bleomycin hydrochloride is an antitumor antibiotic^[1].

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

Alle Antibiotic Isoform-spezifische Produkte anzeigen :

In Vitro

Bleomycin hydrochloride is chosen as the best-studied micronucleus inducers in human lymphocytes with different mechanisms of genotoxicity. The most frequent Bleomycin-induced DNA lesions are single and double strand breaks and single apuinic/apyrimidinic sites. At the same time Bleomycin is true radiomimetic compound, resembling almost completely the genetic effect of ionizing radiation^[1].
The IC₅₀ value of Bleomycin hydrochloride for UT-SCC-19A cell line is 4.0±1.3 nM. UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin; IC₅₀ values are 14.2±2.8 nM and 13.0±1.1 nM, respectively^[2].
Bleomycin hydrochloride (50, 100 μM; for 24, 48 h) induce pulmonary fibrosis in RLE-6TN cell (50 μM) and A549 cell (100 μM)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Animal Model:

Male Fischer 344 rats, 8-10 week old, weighing 150-250 g^[3]
Dosage:

3.5-4 mg/kg
Administration:

Intra-tracheal
Result:

Body weights decreased by day 4 then increased by Day 7 through the end of the study.

Induced Disease Models

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Bleomycin hydrochloride ((Bleomycin hydrochloride and Bleomycin sulfate are equivalent)) can be used to create animal models of pulmonary fibrosis. Intratracheal administration of Bleomycin sulfate (3.5-4.0 mg/kg) on day 0 elicits a treatment response, characterized by a decrease in body weight of the subjects on day 4, followed by recovery and continued growth until the end of the experiment. This treatment also significantly increases the levels of hydroxyproline in lung tissue and the mass of the right caudal lobe of the lung. In 8-week-old male BALB/c mice (weighing approximately 20-30 grams), intratracheal instillation of Bleomycin sulfate at a dose of 5.0 mg/kg/day induces pulmonary fibrosis and promotes the expression of α-SMA and type I collagen. Similarly, in 8-week-old male C57BL/6 mice (average weight around 24.5 grams), intratracheal injection of 2.5 mg/kg Bleomycin sulfate effectively induces pulmonary fibrosis. Bleomycin sulfate is rapidly absorbed after administration via intramuscular, subcutaneous, intraperitoneal, or intrapleural routes, reaching peak plasma concentrations within approximately 60 minutes. When given intravenously, less than 1% of the drug binds to plasma proteins, ensuring high bioavailability. The mean plasma clearance rate of Bleomycin sulfate is about 70 mL/min/m², indicating high plasma elimination and urinary excretion rates^[3]^[4]^[5]^[6].

Induction of Pulmonary Fibrosis^[7]

Background

Bleomycin sulfate can lead to lung patchy parenchymal inflammation, epithelial cell injury with reactive hyperplasia, epithelial-mesenchymal transition, activation and differentiation of fibroblasts to myofibroblasts, and basement membrane and alveolar epithelium injures. The experimental use of Bleomycin sulfate is to induce pulmonary fibrosis animal models.

Specific Modeling Methods

Mice: C57BL/6 • 12-week-old
Administration: 3-5 mg/kg • intratracheal administration • sprays on day one

Note

The mice were housed in separate stainless-steel cages (six mice per cage) in a temperature-controlled environment (20-24°C) on 12 h light-dark cycles with unrestricted access to food and water.

Modeling Indicators

Body quality changes: The appetite activity is reduced, with the fur less shiny, the spirits being lethargic, and the bodyweight decreasing. Showed shortness of breath, coughing, and noisy.
Lung changes: Increased fibrotic consolidations, non-aerated lung area, and high-density lung area. Pulmonary function decreased.
Molecular changes: Increased indicators: TGF-β1, TNF-α, IL-6, and GM-CSF in bronchoalveolar lavage fluid.

Correlated Product(s): Bleomycin hydrochloride (HY-17565A)

Opposite Product(s): Neotuberostemonine (HY-N3196)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 67763-87-5
Appearance Solid
Color White to light yellow
SMILES

O=C(C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C)N[C@H](C(N[C@H](C)[C@@H](O)[C@H](C)C(N[C@]([C@H](O)C)([H])C(NCCC2=NC(C3=NC(C(N[R])=O)=CS3)=CS2)=O)=O)=O)[C@H](C4=CN=CN4)O[C@H]5[C@H]([C@H]([C@@H]([C@@H](O5)CO)O)O)O[C@]6([H])[C@H]([C@H]([C@@H]([C@H](O6)CO)O)OC(N)=O)O.NC(NCCCC[R])=N.[R]CCC[S+](C)C.[H]Cl.[R=].[Bleomycin A2].[R=].[Bleomycin B2]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (212)

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Cell Res

Sensing of DNA double-strand breaks by the NHEJ system stabilizes RORγt transcriptional activity and shapes Th17 pathogenicity in autoimmunity. [Abstract]

2026 Jan 7. PMID: 41495483
Nat Metab

The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. [Abstract]

2021 Dec;3(12):1706-1726. PMID: 34873338
Nat Cell Biol

Full-length GSDME mediates pyroptosis independent from cleavage. [Abstract]

2024 Sep;26(9):1545-1557. PMID: 38997456
Drug Resist Updat

ZBP1 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in ovarian cancer. [Abstract]

2026 Jan:84:101319. PMID: 41192279
Nat Commun

Age mosaic of gut epithelial cells prevents aging. [Abstract]

2025 Jul 22;16(1):6734. PMID: 40695820
Nat Commun

A Glb1-2A-mCherry reporter monitors systemic aging and predicts lifespan in middle-aged mice. [Abstract]

2022 Nov 17;13(1):7028. PMID: 36396643
Exp Mol Med

GRIM-19-mediated induction of mitochondrial STAT3 alleviates systemic sclerosis by inhibiting fibrosis and Th2/Th17 cells. [Abstract]

2024 Dec;56(12):2739-2746. PMID: 39643607
ACS Nano

Dissecting Discoidin Domain Receptor 2 Dynamics in Fibrosis with a Programmable Collagen-Mimetic Probe. [Abstract]

2026 Jun 23;20(24):17518-17535. PMID: 42246507
ACS Nano

Targeted Collagen Degradation by an MRI Probe Facilitates siRNA Delivery for Sequential Theranostics in Pulmonary Fibrosis. [Abstract]

2025 Apr 2. PMID: 40173291
J Adv Res

Plasma apolipoprotein E protein attenuates pulmonary fibrosis through LRP1 and PLAU dual receptor-mediated TGF-β/Smad inhibition. [Abstract]

2025 Dec 29:S2090-1232(25)01035-5. PMID: 41475664
J Adv Res

Elevated lactate production exacerbates PM2.5-induced pulmonary fibrosis by stabilizing TGF-β1. [Abstract]

2025 Aug 5:S2090-1232(25)00587-9. PMID: 40759348
J Adv Res

Kynurenine acts as a signaling molecule to attenuate pulmonary fibrosis by enhancing the AHR-PTEN axis. [Abstract]

2024 Jun 19:S2090-1232(24)00254-6. PMID: 38906325
Redox Biol

Pharmaceutical targeting of succinate dehydrogenase in fibroblasts controls bleomycin-induced lung fibrosis. [Abstract]

2021 Oct:46:102082. PMID: 34343908
Mol Cell

Lactylation of XLF promotes non-homologous end-joining repair and chemoresistance in cancer. [Abstract]

2025 Jul 17;85(14):2654-2672.e7. PMID: 40680721
J Biomed Sci

Doublecortin-like kinase 1 promotes fibroblast activation and fibrotic progression through Smad3 binding in idiopathic pulmonary fibrosis. [Abstract]

2026 May 22;33(1):52. PMID: 42174588
MedComm (2020)

Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein. [Abstract]

2023 Jul 12;4(4):e319. PMID: 37457658
Cell Rep Med

Cross-species tropism of AAV.CPP.16 in the respiratory tract and its gene therapies against pulmonary fibrosis and viral infection. [Abstract]

2025 May 14:102144. PMID: 40409263
J Control Release

ROS-responsive microneedles loaded with integrin avβ6-blocking antibodies for the treatment of pulmonary fibrosis. [Abstract]

2023 Aug:360:365-375. PMID: 37331606
Cell Death Dis

Inhibition of Rho GEFs attenuates pulmonary fibrosis through suppressing myofibroblast activation and reprogramming profibrotic macrophages. [Abstract]

2025 Apr 11;16(1):278. PMID: 40216763
Cell Mol Biol Lett

Single-cell RNA sequencing identifies inherent abnormalities of adipose-derived stem cells from nonlesional sites of patients with localized scleroderma. [Abstract]

2024 Aug 30;29(1):115. PMID: 39215271
Cell Death Dis

DEPTOR is a direct p53 target that suppresses cell growth and chemosensitivity. [Abstract]

2020 Nov 12;11(11):976. PMID: 33184290
Cell Death Dis

Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. [Abstract]

2020 Nov 13;11(11):978. PMID: 33188176

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]

Bleomycin (BLM) (5 mg/kg; intratracheal instillation) significantly elevated the ratio of phosphorylated p65 to total p65 (p-p65/p65) and reduced the expression level of IκBα, indicating that NF-κB signaling pathway was activated in BLM-induced pulmonary fibrosis.

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]

Bleomycin (BLM) (5 mg/kg; intratracheal instillation) increases the expression of NLRP3 in BALB/c mice.

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Nov 13;11(11):978. [Abstract]

Bleomycin (BLM) (5 mg/kg; intratracheal instillation) upregulates the mRNA levels of NLRP3, ASC, IL-1β, and IL-18 in BALB/c mice.
Cell Death Dis

Oncogene PRR14 promotes breast cancer through activation of PI3K signal pathway and inhibition of CHEK2 pathway. [Abstract]

2020 Jun 15;11(6):464. PMID: 32541902
Cancer Lett

N6-methyadenosine modified SUV39H2 regulates homologous recombination through epigenetic repression of DUSP6 in gastric cancer. [Abstract]

2023 Apr 1:558:216092. PMID: 36806557
Cancer Lett

DAB2IP suppresses tumor malignancy by inhibiting GRP75-driven p53 ubiquitination in colon cancer. [Abstract]

2022 Apr 28:532:215588. PMID: 35150809
Small

Metal-Polyphenol-Network Coated Prussian Blue Nanoparticles for Synergistic Ferroptosis and Apoptosis via Triggered GPX4 Inhibition and Concurrent In Situ Bleomycin Toxification. [Abstract]

2021 Nov;17(47):e2103919. PMID: 34623753
Phytomedicine

Identification of key anti-pulmonary fibrosis components in Hyssopus cuspidatus Boriss. and its mechanism exploration. [Abstract]

2025 Dec 5:150:157676. PMID: 41389405
Phytomedicine

Network-guided discovery of Zeqi Decoction as a PPARγ-targeted therapy for pulmonary fibrosis. [Abstract]

2025 Dec 16:150:157717. PMID: 41447837
Phytomedicine

Rottlerin inhibits PKCδ to attenuate pulmonary fibrosis by suppressing NLRC4/ASC-mediated pyroptosis. [Abstract]

2025 Oct:146:157154. PMID: 40819638
Phytomedicine

Microbial signatures of areca nut chewing: Bridging the gap between oral dysbiosis and submucous fibrosis. [Abstract]

2025 Sep 4:147:157227. PMID: 40934758
Phytomedicine

Curcumin analogue EF24 prevents alveolar epithelial cell senescence to ameliorate idiopathic pulmonary fibrosis via activation of PTEN. [Abstract]

2024 Jul 27:133:155882. PMID: 39096545
Phytomedicine

Physalis Calyx seu Fructus inhibited pulmonary fibrosis through regulating Wnt/β-catenin signaling pathway. [Abstract]

2024 Jun 3:131:155797. PMID: 38878326

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797. [Abstract]

PCF-EtOH diminished BLM (Bleomycin sulfate)-induced increase of fibronectin, vimentin and α-SMA. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 3:131:155797. [Abstract]

PCF-EtOH blocked BLM (Bleomycin sulfate)-induced upregulation of β-catenin protein. All mice, except those in the control group (nebulization with saline), are nebulised with BLM/saline (1 mg/mL, 8 mL) for 30 min on days 1, 4 and 7, respectively.
Phytomedicine

Vincamine as an agonist of G protein-coupled receptor 40 effectively ameliorates pulmonary fibrosis in mice. [Abstract]

2023 Sep:118:154919. PMID: 37392673
Phytomedicine

Qimai Feiluoping decoction inhibits mitochondrial complex I-mediated oxidative stress to ameliorate bleomycin-induced pulmonary fibrosis. [Abstract]

2023 Apr:112:154707. PMID: 36805483
Adv Healthc Mater

Bioinspired Inhalable Nanoparticles Combining Chinese Herbal Tetrandrine with Pirfenidone Overcome Idiopathic Pulmonary Fibrosis via Coordinated Collagen Modulation and Fibroblast Autophagy Regulation. [Abstract]

2025 Aug 21:e01916. PMID: 40842231
Acta Biomater

Inhaled biomimetic nanoparticles enhance pulmonary delivery of tetrandrine and pirfenidone for the treatment of idiopathic pulmonary fibrosis. [Abstract]

2025 Dec 19:S1742-7061(25)00946-8. PMID: 41423167
J Transl Med

Vitamin D attenuates the progression of pulmonary fibrosis via inhibiting thymidine kinase 1/PFKFB3-driven glycolysis. [Abstract]

2026 Apr 22;24(1):571. PMID: 42021376
J Transl Med

Ficolin-1 ameliorates pulmonary fibrosis via directly binding to TGF-β1. [Abstract]

2024 Nov 21;22(1):1051. PMID: 39574172
J Transl Med

Deciphering the cellular and molecular landscape of pulmonary fibrosis through single-cell sequencing and machine learning. [Abstract]

2025 Jan 2;23(1):3. PMID: 39748378
J Transl Med

MicroRNA-21a-5p inhibition alleviates systemic sclerosis by targeting STAT3 signaling. [Abstract]

2024 Apr 1;22(1):323. PMID: 38561750
Sci China Life Sci

Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization. [Abstract]

2023 Nov;66(11):2571-2586. PMID: 37340175

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Nov;66(11):2571-2586. [Abstract]

Bleomycin (1 mg/kg; single intratracheal injection). Results for time-course Western blot analysis of Plekhf1 and Arg1 (a macrophage M2 marker) and Col 1α (a fibrotic marker) expression in lung macrophages isolated from BLM-treated mice. Left panel: Representative Western blot images. Right panel: Bar graphs showing the data from three replicates.

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Nov;66(11):2571-2586. [Abstract]

Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in lung sections.

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2023 Nov;66(11):2571-2586. [Abstract]

Bleomycin (1 mg/kg; single intratracheal injection). Representative results of coimmunostaining for Plekhf1 and F4/80 in BALF cells from BLM-treated mice.
Int J Biol Macromol

Role of ionizing radiation activated NRF2 in lung cancer radioresistance. [Abstract]

2023 Jun 30:241:124476. PMID: 37076059
JACC Basic Transl Sci

Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling. [Abstract]

2025 Jul;10(7):101255. PMID: 40310323
Sens Actuators B Chem

2025 Sep 4;446:138682.
Mol Med

Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein. [Abstract]

2025 Jun 9;31(1):222. PMID: 40484931
Mol Med

LncRNA GAS5 suppresses TGF-β1-induced transformation of pulmonary pericytes into myofibroblasts by recruiting KDM5B and promoting H3K4me2/3 demethylation of the PDGFRα/β promoter. [Abstract]

2023 Mar 14;29(1):32. PMID: 36918759
Antioxidants (Basel)

Pitavastatin Calcium Confers Fungicidal Properties to Fluconazole by Inhibiting Ubiquinone Biosynthesis and Generating Reactive Oxygen Species. [Abstract]

2024 May 29;13(6):667. PMID: 38929106
Phytother Res

Bryodulcosigenin attenuates bleomycin-induced pulmonary fibrosis via inhibiting AMPK-mediated mesenchymal epithelial transition and oxidative stress. [Abstract]

2022 Oct;36(10):3911-3923. PMID: 35794782
Stem Cell Res Ther

A scalable platform for EPSC-Induced MSC extracellular vesicles with therapeutic potential. [Abstract]

2025 Aug 5;16(1):426. PMID: 40765003
Stem Cell Res Ther

hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1. [Abstract]

2025 Jan 5;16(1):2. PMID: 39757225
ACS Appl Mater Interfaces

The Clearance Effect of Tetrahedral DNA Nanostructures on Senescent Human Dermal Fibroblasts. [Abstract]

2019 Jan 16;11(2):1942-1950. PMID: 30562007
Cell Rep

Fibroblast MrgprX2/B2 signaling drives hypertrophic scar fibrosis. [Abstract]

2026 Jun 25;45(7):117616. PMID: 42360879
Aging Cell

Pck1 Deficiency Drives Mitochondrial Dysfunction and Cellular Senescence in Adipocytes. [Abstract]

2026 Apr;25(4):e70462. PMID: 41910356
Cell Rep

Lactylation-driven nuclear RIG-I promoted by lactate transporter inhibitor suppresses DNA damage repair through inhibiting PARP1 activity. [Abstract]

2026 Jan 13;45(1):116854. PMID: 41538326
Cell Rep

Phosphatase PHLPP1 is an alveolar-macrophage-intrinsic transcriptional checkpoint controlling pulmonary fibrosis. [Abstract]

2025 Mar 12;44(3):115399. PMID: 40085643
Cell Rep

Mitotic ER-mitochondria contact enhances mitochondrial Ca2+ influx to promote cell division. [Abstract]

2024 Sep 28;43(10):114794. PMID: 39342616
Chin Med

TMT proteomics analysis reveals the mechanism of bleomycin-induced pulmonary fibrosis and effects of Ginseng honeysuckle superfine powdered tea. [Abstract]

2023 May 24;18(1):60. PMID: 37221600
J Med Chem

Discovery and Evaluation of a PROTAC Degrader Targeting SAMHD1 for the Treatment of Pulmonary Fibrosis. [Abstract]

2026 Apr 23;69(8):9363-9385. PMID: 41932858
Anal Chem

Sequential Labeling-Assisted Precise and Multitarget Analysis of Surface Proteins on Extracellular Vesicles. [Abstract]

2025 Jun 3;97(21):11073-11081. PMID: 40388194
J Med Chem

Identification of Nitric Oxide Donating Dasatinib Derivatives with Intraocular Pressure Lowering and Senolytic Activities. [Abstract]

2025 Apr 14. PMID: 40228166
J Invest Dermatol

Excess KLHL24 impairs skin wound healing by degradation of vimentin. [Abstract]

2023 Jul;143(7):1289-1298.e15. PMID: 36716923
Fundam Res

Pharmacological regulation of tissue fibrosis by targeting the mechanical contraction of myofibroblasts. [Abstract]

2021 Dec 23;2(1):37-47. PMID: 38933917
Drug Deliv Transl Res

Propellant-free intrawound antibiotic foam for intraoperative antimicrobial prophylaxis. [Abstract]

2026 Jul;16(7):2514-2526. PMID: 42050315
Redox Rep

Prophylactic quercetin administration attenuates pulmonary fibrosis via ferroptosis-resistant priming of alveolar epithelial cells. [Abstract]

2026 Dec;31(1):2632434. PMID: 41697789
J Ethnopharmacol

Study on the anti-pulmonary fibrosis mechanism of Renshen Pingfei formula by inhibiting M2 macrophage polarization and regulating TGF - β/SMAD signaling pathway. [Abstract]

2026 May 23:363:121465. PMID: 41780616
J Ethnopharmacol

A novel polysaccharide from Qamgur (Brassica rapa L.) mitigates pulmonary fibrosis by regulating inflammatory, epithelial-mesenchymal transition, and fibrotic pathways. [Abstract]

2026 Apr 6:360:121174. PMID: 41500353
J Ethnopharmacol

Mimosa pudica L. extract ameliorates pulmonary fibrosis via modulation of MAPK signaling pathways and FOXO3 stabilization. [Abstract]

2024 Aug 10:330:118226. PMID: 38670401
Ecotoxicol Environ Saf

PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]

2025 Nov 15:307:119433. PMID: 41273832
Biochem Pharmacol

Targeting PBK in the fibroblast-to-myofibroblast transition: A novel therapeutic strategy for IPF. [Abstract]

2025 Aug 28;242(Pt 2):117283. PMID: 40885317
Biochem Pharmacol

NLRP3 inflammasome activation contributes to the development of the pro-fibrotic phenotype of lung fibroblasts. [Abstract]

2024 Aug 17:116496. PMID: 39159876
Cell Mol Life Sci

Role of transient receptor potential ankyrin 1 in idiopathic pulmonary fibrosis: modulation of M2 macrophage polarization. [Abstract]

2024 Apr 18;81(1):187. PMID: 38635081

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187. [Abstract]

Bleomycin sulfate (3 mg/kg; nebulized) significantly upregulates TRPA1 protein levels in mice.

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2024 Apr 18;81(1):187. [Abstract]

Bleomycin sulfate (3 mg/kg; nebulized) significantly increases TRPA1 mRNA levels in mouse lung tissues.
Mech Ageing Dev

Calcium transferring from ER to mitochondria via miR-129/ITPR2 axis controls cellular senescence in vitro and in vivo. [Abstract]

2024 Apr:218:111902. PMID: 38218462

Bleomycin hydrochloride purchased from MedChemExpress. Usage Cited in: Mech Ageing Dev. 2024 Apr:218:111902. [Abstract]

miR-129 delays bleomycin-induced cellular senescence. MRC5 cells were transfected with miR-Ctrl or miR-129 and then treated with bleomycin (1 µg/ml) where indicated. Four days after transfection, the p-H2AX, ITPR2, p16, p21 and GAPDH protein levels were quantified by western blot.
Biochem Pharmacol

Nrf2 in keratinocytes protects against skin fibrosis via regulating epidermal lesion and inflammatory response. [Abstract]

2020 Apr;174:113846. PMID: 32032580
Life Sci

Autophagy-related protein EI24 delays the development of pulmonary fibrosis by promoting autophagy. [Abstract]

2021 Jan 1;264:118664. PMID: 33127511
Mol Metab

DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes. [Abstract]

2022 Aug:62:101524. PMID: 35660116
OncoImmunology

Pirfenidone facilitates immune infiltration and enhances the antitumor efficacy of PD-L1 blockade in mice. [Abstract]

2020 Sep 22;9(1):1824631. PMID: 33457101
Drug Des Devel Ther

Integrated Serum Pharmacochemistry, Network Pharmacology, and Transcriptomics Reveal the Mechanisms and Active Constituents of Qingfei Huoxue Decoction Against Bleomycin-Induced Pulmonary Fibrosis. [Abstract]

2026 Apr 7:20:592770. PMID: 41970211
Transl Res

Dach1 deficiency drives alveolar epithelium apoptosis in pulmonary fibrosis via modulating C-Jun/Bim activity. [Abstract]

2023 Jul:257:54-65. PMID: 36754276
J Cell Biol

DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair. [Abstract]

2021 Feb 1;220(2):e201911025. PMID: 33404607
Cells

ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1-BLM Interaction. [Abstract]

2022 Dec 29;12(1):145. PMID: 36611939
Cells

Beyond the Warburg Effect: Oxidative and Glycolytic Phenotypes Coexist within the Metabolic Heterogeneity of Glioblastoma. [Abstract]

2021 Jan 20;10(2):202. PMID: 33498369
Commun Biol

Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. [Abstract]

2025 Jan 14;8(1):51. PMID: 39809890
Eur J Pharmacol

Miltefosine attenuates silicosis involving inhibiting the Akt/mTOR signaling pathway. [Abstract]

2026 May 27:178931. PMID: 42203065
Respir Res

Dehydrocorydaline attenuates bleomycin-induced pulmonary fibrosis by inhibiting fibroblast activation. [Abstract]

2025 Apr 12;26(1):136. PMID: 40221718
Pharmaceuticals (Basel)

Comparison of the Results of Modeling Pulmonary Fibrosis in Sprague Dawley Rats by Intratracheal Administration of Bleomycin in the Form of Sulfate and Chloride at a Dose of 3 mg/kg. [Abstract]

2024 Oct 11;17(10):1360. PMID: 39459000
Respir Res

MiR-125b-5p alleviates pulmonary fibrosis by inhibiting TGFβ1-mediated epithelial-mesenchymal transition via targeting BAK1. [Abstract]

2024 Oct 19;25(1):382. PMID: 39427175
Eur J Pharmacol

Carbon monoxide attenuates cellular senescence-mediated pulmonary fibrosis via modulating p53/PAI-1 pathway. [Abstract]

2024 Jul 26:176843. PMID: 39068977
Clin Epigenetics

Histone deacetylase 3 deletion in alveolar type 2 epithelial cells prevents bleomycin-induced pulmonary fibrosis. [Abstract]

2023 Nov 11;15(1):182. PMID: 37951958
Respir Res

Long non-coding RNA H19 deficiency ameliorates bleomycin-induced pulmonary inflammation and fibrosis. [Abstract]

2020 Nov 2;21(1):290. PMID: 33138822
Respir Res

Curdione ameliorates bleomycin-induced pulmonary fibrosis by repressing TGF-β-induced fibroblast to myofibroblast differentiation. [Abstract]

2020 Feb 19;21(1):58. PMID: 32075634
Biomolecules

Notch Signaling Exacerbates Pulmonary Fibrosis by Regulating the Differentiation of CD4+ Tissue-Resident Memory T Cells. [Abstract]

2026 Feb 20;16(2):328. PMID: 41750395
Int J Mol Sci

Integrating Network Pharmacology, Machine Learning, and Experimental Validation to Elucidate the Mechanism of Cardamonin in Treating Idiopathic Pulmonary Fibrosis. [Abstract]

2025 Dec 25;27(1):249. PMID: 41516127
Int Immunopharmacol

BI-2536 attenuates IPF progression by inhibiting the PLK2/JNK/SP1 Signaling pathway in AT2 cells. [Abstract]

2026 Jan 1;168(Pt 1):115812. PMID: 41237696
Int Immunopharmacol

JTE-013 reduces inflammation and improves pulmonary fibrosis by regulating the TRAF2/NF - κB/IGF-1 signaling pathway and macrophage polarization. [Abstract]

2025 Oct 30:167:115727. PMID: 41172817
Int Immunopharmacol

Six1 promotes alveolar epithelium senescence in pulmonary fibrosis through regulating Tp53. [Abstract]

2025 Sep 23:166:115554. PMID: 40991995
Int Immunopharmacol

CCTα and GVI iPLA2-induced aberrant phosphatidylcholine metabolism contributes to pulmonary inflammation and fibrosis. [Abstract]

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Int Immunopharmacol

SLAMF7-expressing B cells as crucial mediators in the pathogenesis of rheumatoid arthritis-interstitial lung disease. [Abstract]

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Int Immunopharmacol

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Int Immunopharmacol

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Int Immunopharmacol

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2023 Jul:120:110369. PMID: 37224650
Int Immunopharmacol

Effects of Ruxolitinib on fibrosis in preclinical models of systemic sclerosis. [Abstract]

2023 Mar:116:109723. PMID: 36696855
Int Immunopharmacol

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2022 Dec;113(Pt A):109409. PMID: 36461602
Inflammation

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2024 Oct;47(5):1744-1761. PMID: 38498270
Inflammation

Nintedanib Ameliorates Bleomycin-Induced Pulmonary Fibrosis, Inflammation, Apoptosis, and Oxidative Stress by Modulating PI3K/Akt/mTOR Pathway in Mice. [Abstract]

2023 Aug;46(4):1531-1542. PMID: 37160579
Front Pharmacol

TRAF6 Suppresses the Development of Pulmonary Fibrosis by Attenuating the Activation of Fibroblasts. [Abstract]

2022 May 20;13:911945. PMID: 35668944
Am J Respir Cell Mol Biol

DOCK2 Promotes Pleural Fibrosis by Modulating Mesothelial to Mesenchymal Transition. [Abstract]

2022 Feb;66(2):171-182. PMID: 34710342
Am J Respir Cell Mol Biol

Clioquinol Attenuates Pulmonary Fibrosis through Inactivation of Fibroblasts via Iron Chelation. [Abstract]

2021 Aug;65(2):189-200. PMID: 33861690
Am J Respir Cell Mol Biol

Kindlin-2 Acts as a Key Mediator of Lung Fibroblast Activation and Pulmonary Fibrosis Progression. [Abstract]

2021 Jul;65(1):54-69. PMID: 33761308
Molecules

Cepharanthine Ameliorates Pulmonary Fibrosis by Inhibiting the NF-κB/NLRP3 Pathway, Fibroblast-to-Myofibroblast Transition and Inflammation. [Abstract]

2023 Jan 11;28(2):753. PMID: 36677811
Biochim Biophys Acta Mol Basis Dis

Integrated bioinformatics and machine learning analysis identify CROT as a regulator of immunological features in idiopathic pulmonary fibrosis. [Abstract]

2026 Feb;1872(2):168100. PMID: 41192648
Biochim Biophys Acta Mol Basis Dis

MBD2 promotes epithelial-to-mesenchymal transition (EMT) and ARDS-related pulmonary fibrosis by modulating FZD2. [Abstract]

2025 Mar 12;1871(5):167798. PMID: 40081619
Sci Rep

Targeting colorectal cancer with pyrazolo[4,3-e][1,2,4]triazine and pyrazolo[4,3-e]tetrazolo[1,5-b][1,2,4]triazine sulfonamides: comprehensive in vitro evaluation. [Abstract]

2026 May 31. PMID: 42225755
Sci Rep

Rubiadin-1-methyl ether alleviates bleomycin induced pulmonary fibrosis. [Abstract]

2026 Jan 8;16(1):4864. PMID: 41507413
Cell Calcium

2025 Oct 22.
Biol Proced Online

Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening. [Abstract]

2025 Jun 16;27(1):21. PMID: 40524168
Sci Rep

Amifostine attenuates bleomycin-induced pulmonary fibrosis in mice through inhibition of the PI3K/Akt/mTOR signaling pathway. [Abstract]

2023 Jun 28;13(1):10485. PMID: 37380638
Mediators Inflamm

Interleukin-19 Aggravates Pulmonary Fibrosis via Activating Fibroblast through TGF- β/Smad Pathway. [Abstract]

2022 Mar 3:2022:6755407. PMID: 35281428
Eur J Med Res

Tetrathiomolybdate alleviates bleomycin-induced pulmonary fibrosis by reducing copper concentration and suppressing EMT. [Abstract]

2025 May 19;30(1):394. PMID: 40390111
Cancers (Basel)

IOS-1002, a Stabilized HLA-B57 Open Format, Exerts Potent Anti-Tumor Activity. [Abstract]

2024 Aug 21;16(16):2902. PMID: 39199672
J Cell Mol Med

Salvianolic Acid B Inhibited LH2 Expression to Reduce Collagen Synthesis in Pulmonary Fibrosis. [Abstract]

2026 May;30(10):e71168. PMID: 42179136
J Cell Mol Med

Cinobufotalin Ameliorates the Development of Pulmonary Fibrosis by Suppressing the TGF-β/Smad Pathway via Regulating PI15. [Abstract]

2025 Aug;29(16):e70788. PMID: 40845143
Cell Signal

hMOB2 deficiency impairs homologous recombination-mediated DNA repair and sensitises cancer cells to PARP inhibitors. [Abstract]

2021 Nov:87:110106. PMID: 34363951
J Inflamm Res

Qimai Feiluoping Decoction Inhibits EndMT to Alleviate Pulmonary Fibrosis by Reducing PI3K/AKT/mTOR Pathway-Mediated the Restoration of Autophagy. [Abstract]

2025 Jun 26:18:8447-8475. PMID: 40589610
J Inflamm Res

VISTA Deficiency Exacerbates the Development of Pulmonary Fibrosis by Promoting Th17 Differentiation. [Abstract]

2024 Jun 19:17:3983-3999. PMID: 38911987
iScience

LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis. [Abstract]

2026 Jun 4;29(6):116212. PMID: 42305620
iScience

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2026 Mar 30;29(5):115495. PMID: 42004028
Biomedicines

Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects. [Abstract]

2023 Feb 5;11(2):463. PMID: 36830999
FASEB J

Hesperetin Alleviates Bleomycin-Induced Pulmonary Fibrosis by Modulating Cellular Senescence and Promoting Impaired Autophagy in a CISD2-Dependent Manner. [Abstract]

2025 Oct 15;39(19):e71083. PMID: 41001804
J Pharmacol Exp Ther

Disulfiram activation of prostaglandin E2 synthesis: a novel antifibrotic mechanism in pulmonary fibrosis. [Abstract]

2025 Jun;392(6):103588. PMID: 40359874
FEBS J

TRPM7 controls skin keratinocyte senescence by targeting intracellular calcium signaling. [Abstract]

2024 Nov;291(21):4680-4695. PMID: 39185948
Am J Physiol Endocrinol Metab

Secreted GDF15 maintains transcriptional responses during DNA damage-mediated senescence in human beta cells. [Abstract]

2024 Oct 1;327(4):E552-E562. PMID: 39196800
Lab Invest

Senescent Epithelial Cells Serve as Invasive Growth Drivers in Ameloblastoma. [Abstract]

2025 Aug 6;105(11):104227. PMID: 40780542
Front Biosci (Landmark Ed)

CD5L Deficiency Protects Mice Against Bleomycin-Induced Pulmonary Fibrosis. [Abstract]

2023 Sep 22;28(9):209. PMID: 37796694
Naunyn Schmiedebergs Arch Pharmacol

Identification of potential mechanisms of Schisandrin B in the treatment of idiopathic pulmonary fibrosis by integrating network pharmacology and experimental validation. [Abstract]

2025 May;398(5):5389-5403. PMID: 39549058
Naunyn Schmiedebergs Arch Pharmacol

Zerumbone alleviated bleomycin-induced pulmonary fibrosis in mice via SIRT1/Nrf2 pathway. [Abstract]

2024 Nov;397(11):8979-8992. PMID: 38874804
Pathol Res Pract

Combined treatment of tacrolimus and pirfenidone effectively improves BLM-induced pulmonary fibrosis in mice by inhibiting the TGFβ1/Smad2/3 signaling. [Abstract]

2025 Aug:272:156095. PMID: 40617191
Basic Clin Pharmacol Toxicol

7,8-Dihydroxyflavone Suppresses Experimental Pulmonary Fibrosis by Inhibiting Fibroblast-to-Myofibroblast Transformation and Epithelial-Mesenchymal Transition. [Abstract]

2026 Jan;138(1):e70158. PMID: 41347324
Toxicol Appl Pharmacol

Mitochondrial dysfunction-associated cellular senescence is partially involved in bleomycin-induced pulmonary fibrosis in mice. [Abstract]

2025 Aug 19:504:117524. PMID: 40840615
Cytokine

Role of FGF19 in regulating mitochondrial dynamics and macrophage polarization through FGFR4/AMPKα-p38/MAPK Axis in bleomycin-induced pulmonary fibrosis. [Abstract]

2025 Jun 22:193:156978. PMID: 40550190
Stem Cells Int

Transcriptome and Proteome Analysis Identify Decorin as a Principal Antifibrotic Component Trapping TGF- β 1 Within Adipose-Derived Stem Cell Secretome. [Abstract]

2025 May 9:2025:1416567. PMID: 40386129
Appl Biochem Biotechnol

CSE Induces Lung Epithelial Cell-derived Exosomal LINC01133-mediated FOXA1 Activity Through SRSF6 to Affect MRC-5 Cellular Lipid Metabolism and Autophagy. [Abstract]

2026 May;198(5):3954-3974. PMID: 41793647
FEMS Yeast Res

Characterization of oncohistone H2B variants in Schizosaccharomyces pombe reveals a key role of H2B monoubiquitination deficiency in genomic instability by altering gene expression. [Abstract]

2025 May 22:foaf027. PMID: 40402811
Bioorg Med Chem

Design, synthesis, and evaluation of a novel TRAIL-activated HDAC6 inhibitor for the treatment of pulmonary fibrosis. [Abstract]

2024 Nov 1:113:117924. PMID: 39321740
BMC Pharmacol Toxicol

New tricks for old drugs- praziquantel ameliorates bleomycin-induced pulmonary fibrosis in mice. [Abstract]

2024 Feb 14;25(1):18. PMID: 38355586
Immunobiology

HMGB1 regulates the activation of dendritic cells and CD4+ T cell responses through the modulation of autophagy in bleomycin-induced pulmonary fibrosis. [Abstract]

2025 Apr 23;230(3):152906. PMID: 40311346
BMC Pulm Med

A selective CB2R agonist (JWH133) protects against pulmonary fibrosis through inhibiting FAK/ERK/S100A4 signaling pathways. [Abstract]

2023 Nov 13;23(1):440. PMID: 37957604
Toxicol Lett

The MIR100HG/miR-29a-3p/Tab1 axis modulates TGF-β1-induced fibrotic changes in type II alveolar epithelial cells BLM-caused lung fibrogenesis in mice. [Abstract]

2022 Jun 15:363:45-54. PMID: 35472619
Immunopharmacol Immunotoxicol

Atractylenolide III attenuates bleomycin-induced experimental pulmonary fibrosis and oxidative stress in rat model via Nrf2/NQO1/HO-1 pathway activation. [Abstract]

2020 Oct;42(5):436-444. PMID: 32762376
J Gene Med

The CHST11 gene is linked to lung cancer and pulmonary fibrosis. [Abstract]

2022 Sep 30;e3451. PMID: 36181245
Fitoterapia

ZhuYang TongBi decoction ameliorates pulmonary fibrosis by degrading TGFBR via CAV1. [Abstract]

2026 May 21:192:107300. PMID: 42173173
Medicina (Kaunas)

Saroglitazar Ameliorates Pulmonary Fibrosis Progression in Mice by Suppressing NF-κB Activation and Attenuating Macrophage M1 Polarization. [Abstract]

2025 Jun 26;61(7):1157. PMID: 40731787
Biomed Res Int

Protective Effects of the Wenfei Buqi Tongluo Formula on the Inflammation in Idiopathic Pulmonary Fibrosis through Inhibiting the TLR4/MyD88/NF- κ B Pathway. [Abstract]

2022 Feb 7;2022:8752325. PMID: 35178456
Mutat Res Genet Toxicol Environ Mutagen

Role of exposure/recovery schedule in micronuclei induction by several promutagens in V79-derived cells expressing human CYP2E1 and SULT1A1. [Abstract]

2016 Sep 15:808:27-37. PMID: 27637483
Ther Adv Chronic Dis

Scutellarein inhibits BLM-mediated pulmonary fibrosis by affecting fibroblast differentiation, proliferation, and apoptosis. [Abstract]

2020 Jul 30;11:2040622320940185. PMID: 32843954
J Mol Histol

Echinacoside ameliorates bleomycin-induced idiopathic pulmonary fibrosis by regulating macrophage polarization. [Abstract]

2025 Nov 24;57(1):4. PMID: 41284091
Biochem Biophys Res Commun

Macrophage erythropoietin receptor signaling drives macrophage-myofibroblast transition via JAK2/STAT3 and TGF-β1/Smad3 pathways to exacerbate pulmonary fibrosis in mice. [Abstract]

2026 Apr 9:808:153453. PMID: 41719615
Biochem Biophys Res Commun

The hematopoietic activity of EPO is unfavorable to the treatment of bleomycin-induced pulmonary fibrosis in mice. [Abstract]

2024 Nov 4:739:150951. PMID: 39547119
Biochem Biophys Res Commun

Asiaticoside attenuates bleomycin-induced pulmonary fibrosis in A2aR-/- mice by promoting the BMP7/Smad1/5 signaling pathway. [Abstract]

2020 Jun 30;527(3):662-667. PMID: 32423816
Biochem Cell Biol

A novel mouse model of pulmonary fibrosis: twice-repeated oropharyngeal bleomycin administration mimicking human pathology. [Abstract]

2025 Jan 1:103:1-7. PMID: 39878201
Exp Ther Med

MicroRNA‑203a‑3p improves bleomycin and pingyangmycin sensitivity by inactivating the PI3K/AKT pathway in hemangioma. [Abstract]

2024 Jan 4;27(2):80. PMID: 38274341
Can Respir J

Cortex Mori Radicis Mitigates Inflammation and Fibrosis in Pulmonary Fibrosis Through PI3K/AKT Pathway Suppression. [Abstract]

2026;2026(1):e8459298. PMID: 42365453
Curr Med Sci

Therapeutic Effects of Qifangfeixian Granules on Pulmonary Fibrosis in Patients with Interstitial Lung Disease. [Abstract]

2025 Sep 30. PMID: 41026340
In Vivo

Antagonistic Effect of Erythropoietin Receptor Signaling in Macrophages and Type II Alveolar Epithelial Cells on Pulmonary Fibrosis in Mice. [Abstract]

2026 May-Jun;40(3):1517-1529. PMID: 42049419
Rheumatol Autoimmun

2024 Jun 28.
Int J Neurosci

Thymic stromal lymphopoietin modification gates chronic pain via regulation of transient receptor potential vanilloid type 1-caspase1. [Abstract]

2025 Aug 7:1-9. PMID: 40758369
Nat Prod Commun

2024 Mar 29.
STAR Protoc

Protocol for high-throughput viability screening and gene expression of human EndoC-βH5 β cells and pancreatic islets. [Abstract]

2025 Jul 18;6(3):103955. PMID: 40682782
Mol Cell Toxicol

2025 May 4.
Genes Cells

SRPKs Homolog Dsk1 Regulates Homologous Recombination Repair in Schizosaccharomyces pombe. [Abstract]

2025 Jan;30(1):e13192. PMID: 39789818
J Vis Exp

Refined Murine Model of Idiopathic Pulmonary Fibrosis. [Abstract]

2025 Jun 17:(220). PMID: 40622990
J Vis Exp

Unilateral Lung Volume Analysis Using Micro-CT for Enhanced Assessment of Pulmonary Fibrosis in Preclinical Models. [Abstract]

2025 Jun 20:(220). PMID: 40552821
J Vis Exp

Establishment and Validation of a Rat Model of Pulmonary Arterial Hypertension Associated with Pulmonary Fibrosis. [Abstract]

2025 May 23:(219). PMID: 40489445
Gene Rep

2023 Jul 22, 101823.
Res Sq

2026 Jun 16.
SSRN

2026 Jun 18.
SSRN

2026 May 16.
bioRxiv

2026 Apr 30.
Res Sq

2026 Apr 14.
medRxiv

2025 Nov 28.
SSRN

2025 Dec 12.
Res Sq

2025 Oct 30.
SSRN

2025 Oct 21.
SSRN

2025 Sep 3.
Int J Exp Pathol

Porcine respiratory disease complex induces pulmonary fibrosis related to the aberrant sphingolipid metabolism. [Abstract]

2025 Sep;106(5):e70005. PMID: 40884045
bioRxiv

2025 Jul 26.
Libyan International Journal of Natural Sciences

2025 May 26.
bioRxiv

2025 May 09.
Res Sq

2025 May 16.
Research Square Print

2025 May 28.
bioRxiv

2025 March 19.
bioRxiv

2025 March 11.
Res Sq

2024 Oct 31.
bioRxiv

2024 Nov 06.
Heliyon

Integrative analysis of the efficacy and pharmacological mechanism of Xuefu Zhuyu decoction in idiopathic pulmonary fibrosis via evidence-based medicine, bioinformatics, and experimental verification. [Abstract]

2024 Sep 20;10(19):e38122. PMID: 39416822
MedComm Futur Med

2024 Jul 19.
bioRxiv

2024 July 27.
Heliyon

Sinomenine ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the differentiation of fibroblast into myofibroblast. [Abstract]

2024 Jun 24;10(13):e33314. PMID: 39050413
Biomed Pharmacother

Alpinetin ameliorates bleomycin-induced pulmonary fibrosis by repressing fibroblast differentiation and proliferation. [Abstract]

2024 Feb:171:116101. PMID: 38228032
Research Square Preprint

2023 Nov 23.
Heliyon

Cellular and molecular mechanisms of fibrosis and resolution in bleomycin-induced pulmonary fibrosis mouse model revealed by spatial transcriptome analysis. [Abstract]

2023 Nov 20;9(12):e22461. PMID: 38125541
Research Square Preprint

2023 Aug 30.
Research Square Preprint

2023 Aug 4.
Research Square Preprint

2023 Aug 3.
Research Square Print

November 28th, 2022.
Biomed Pharmacother

Iguratimod ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the EMT process and NLRP3 inflammasome activation. [Abstract]

2022 Sep:153:113460. PMID: 36076570
Universidad de las Illes Balears

Facultad de Ciencias, 2022 Feb.
Chinese Journal of Plastic and Reconstructive Surgery

23 December 2021.
Oxid Med Cell Longev

Astragaloside IV Inhibits Bleomycin-Induced Ferroptosis in Human Umbilical Vein Endothelial Cells by Mediating LPC. [Abstract]

2021 Nov 1:2021:6241242. PMID: 34760046
Oxid Med Cell Longev

Gasotransmitter CO Attenuates Bleomycin-Induced Fibroblast Senescence via Induction of Stress Granule Formation. [Abstract]

2021 Jun 29:2021:9926284. PMID: 34306316
Biomed Pharmacother

Dopamine receptor agonists ameliorate bleomycin-induced pulmonary fibrosis by repressing fibroblast differentiation and proliferation. [Abstract]

2021 Jul:139:111500. PMID: 33901873
Patent

US20190282565A1.
Patent

US20190255042A1.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 100 mg/mL (Need ultrasonic)

DMSO : 50 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

In Vivo:

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Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL; Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

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Verweise

[1]. Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49. [Content Brief]

[2]. Jaaskela-Saari HA, et al. Squamous cell cancer cell lines: sensitivity to bleomycin and suitability for animal xenograft studies. Acta Otolaryngol Suppl. 1997;529:241-4. [Content Brief]

[3]. Corboz MR, et al. Therapeutic administration of inhaled INS1009, a LRX-15 prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103. [Content Brief]

[4]. Kang Miao, et al. Scutellarein inhibits BLM-mediated pulmonary fibrosis by affecting fibroblast differentiation, proliferation, and apoptosis. Ther Adv Chronic Dis. 2020 Jul 30;11:2040622320940185. [Content Brief]

[5]. Ling Peng, et al. Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial-mesenchymal transition and inflammation. Cell Death Dis. 2020 Nov 13;11(11):978. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Bleomycin hydrochloride

212 Cited Publications

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Bleomycin hydrochloride

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Biologische Aktivität

Chemical Information

Publications (212)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Bleomycin hydrochloride

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

FDA-Approved Drug Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Drug Repurposing Compound Library

NMPA-Approved Drug Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Anti-Pancreatic Cancer Compound Library

Animal Disease Model Inducer Library

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EMA-Approved Drug Library

FDA-Approved Anticancer Drug Library

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High-Throughput Bioactive Compound Library

Anti-Cancer Approved Drug Library

Publications Citing Use of MedChemExpress (MCE) Bleomycin hydrochloride

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (212)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

Bleomycin hydrochloride Related Classifications

HY-17565A Related Classifications