WC-27
WC-27 is a selective Wee1 inhibitor with an IC50 of 2.11 nM. WC-27 blocks Wee1-mediated inhibitory phosphorylation of CDK1 and disrupts the G2/M checkpoint. WC-27 induces S-phase cell cycle arrest and apoptosis, and inhibits cell migration and colony formation. WC-27 can be used for research on non-small cell lung cancer.
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- CAS No.: 3069940-08-2
- Formule: C22H25Cl2N7O2
- Masse moléculaire:490.39
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
[1]|
WEE1 2.11 nM (IC50) |
CDK1 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| NCI-H1299 | IC50 |
1.05 μM
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Antiproliferative activity against human NCI-H1299 non-small cell lung cancer cells assessed as viability reduction incubated 72 hrs by MTT assay.
Antiproliferative activity against human NCI-H1299 non-small cell lung cancer cells assessed as viability reduction incubated 72 hrs by MTT assay.
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42887067 |
| LoVo | IC50 |
0.38 μM
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Antiproliferative activity against human LOVO cells assessed as viability reduction incubated 72 hrs by MTT assay.
Antiproliferative activity against human LOVO cells assessed as viability reduction incubated 72 hrs by MTT assay.
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42887067 |
| HCC1569 | IC50 |
0.79 μM
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Antiproliferative activity against human HCC1569 cells assessed as viability reduction incubated 72 hrs by MTT assay.
Antiproliferative activity against human HCC1569 cells assessed as viability reduction incubated 72 hrs by MTT assay.
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42887067 |
In Vitro
WC-27 (72 h) inhibits LOVO and HCC1569 cell proliferation with IC50 values of 0.38 µM and 0.79 µM, respectively[1].
WC-27 (2.5 μM) inhibits phosphorylation of CDK1 at Tyr15 in NCI-H1299 cells[1].
WC-27 (0.625-5.0 μM; 24 h) inhibits the migration of NCI-H1299 cells in a concentration-dependent manner[1].
WC-27 (0.625-1.25 μM; 10 days) inhibits colony formation of NCI-H1299 cells in a concentration-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NCI-H1299 cells
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Concentration:0.625, 1.25, 2.5, and 5.0 µM
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Incubation Time:72 h
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Result:S-phase cells: 15.24% at 0.625 µM.
S-phase cells: 21.39% at 1.25 µM.
S-phase cells: 31.39% at 2.5 µM.
S-phase cells: 51.93% at 5.0 µM.
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Cell Line:NCI-H1299 cells
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Concentration:0.625-5.0 µM
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Incubation Time:24 h
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Result:Significantly inhibited wound closure of NCI-H1299 cells in a concentration-dependent manner.
Wound healing rate markedly decreased as concentration increased from 0.625 µM to 5.0 µM.
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Cell Line:NCI-H1299 cells
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Concentration:0.625 and 1.25 µM
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Incubation Time:10 days
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Result:Colony formation rates decreased significantly.
Near-complete inhibition of colony formation was observed at 0.625 µM.
Almost no colonies formed at 1.25 µM.
- Western Blot
- Scratch/Wound-Healing Migration Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Cell migration
- Neural Crest/Neuronal Cell Migration Explant Assay
- Protocol for Cell Cycle
- Colony Formation (Clonogenic) Assay
- Kinase activity and phosphorylation assays
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Zigmond Chamber Chemotaxis Assay
- Under-Agarose Cell Migration Assay
- Transwell/Boyden Chamber Migration Assay
- Apoptosis Solutions
- 3D Collagen/Hydrogel Matrix Migration Assay
- Protocol for Kinase activity and phosphorylation assays
- Soft Agar Colony Formation Assay
- Cell-Exclusion Zone Migration Assay
- Dunn/Insall Chamber Chemotaxis Assay
Parmacokinetics
Chemical Information
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CAS No. 3069940-08-2
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Masse moléculaire 490.39
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Formule C22H25Cl2N7O2
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SMILES
O=C(NC1=C(Cl)C=CC=C1Cl)C2=CN=C(NC3=CN(C4CCN(C)CC4)N=C3)N=C2OCC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
WC-27 Related Classifications
HY-190477 Related Classifications
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