2. PI3K/Akt/mTOR Apoptosis Metabolic Enzyme/Protease
  3. mTOR Apoptosis Mitochondrial Metabolism PI3K Akt
  4. COH-19

COH-19 is a potent mTOR inhibitor. COH-19 binds to the ATP-binding pocket of mTOR, reduces mTOR protein levels, and suppresses the PI3K/Akt/mTOR signaling pathway. COH-19 induces apoptosis via the mitochondrial pathway. COH-19 causes G0/G1 phase cell cycle arrest and suppresses cancer cell proliferation. COH-19 can be used for the research of breast cancer.

For research use only. We do not sell to patients.

COH-19

COH-19 Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

COH-19 Related Antibodies

Top Publications Citing Use of Products

View All mTOR Isoform Specific Products:

View All Isoforms
mTOR mTORC1 mTORC2

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

COH-19 is a potent mTOR inhibitor. COH-19 binds to the ATP-binding pocket of mTOR, reduces mTOR protein levels, and suppresses the PI3K/Akt/mTOR signaling pathway. COH-19 induces apoptosis via the mitochondrial pathway. COH-19 causes G0/G1 phase cell cycle arrest and suppresses cancer cell proliferation. COH-19 can be used for the research of breast cancer[1].

In Vitro

COH-19 (24 h) potently inhibits the viability of C6, MG-63, A549, MCF-7, MDA-MB-231 and MCF-10A cells with IC50 values of 18.52, 19.02, 29.02, 17.02, 26.35, and 40.39 μg/mL, respectively[1].
COH-19 (5-25 μg/mL; 24 h) dose-dependently reduces mTOR protein levels in MCF-7 cells[1].
COH-19 (5-25 μg/mL) dose-dependently disrupts membrane integrity in MCF-7 cells, resulting in low cytotoxicity at 5 μg/mL, medium-high cytotoxicity at 10 μg/mL, and high cytotoxicity (80.6%) at 25 μg/mL[1].
COH-19 (15 μg/mL) upregulates pro-apoptotic and tumor suppressor genes (BRCA1, BRCA2, PTEN, TP53, BAX, CASPASE-3, BRAD1) and downregulates PI3K/AKT pathway genes (PI3K, AKT) in MCF-7 cells, while having no significant effect on BCL-2 expression[1].
COH-19 exerts pro-apoptotic effects in MCF-7 cells by increasing BAX and p53 protein levels and decreasing BCL-2 protein levels, while leaving caspase-3 levels unchanged[1].
COH-19 (10-20 μg/mL) induces chromatin decondensation in MCF-7 cells, indicating altered gene expression profiles[1].
COH-19 (10-25 μg/mL) dose-dependently induces apoptosis in MCF-7 cells凋亡[1].
COH-19 binds stably to the ATP-binding pocket of mTOR (PDB ID: 4JT5) with a binding energy of -7.65 kcal/mol[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

COH-19 Related Antibodies

ELISA Assay[1]

Cell Line: MCF-7
Concentration: 5, 15, 25 μg/mL
Incubation Time: 24 h
Result: Resulted in mTOR protein levels of 0.4341 ng/mL at 5 μg/mL, 0.2740 ng/mL at 15 μg/mL, and 0.1219 ng/mL at 25 μg/mL.
Caused a statistically significant dose-dependent decrease in mTOR protein levels across the three doses.
Reduced mTOR expression by approximately 86% at the IC50 dose.
Molecular Weight

458.43

Formula

C27H17F3N2O2
SMILES

O=C(C1=CC=C2N=C(C3=CC=C(C4=CC=CC=C4)C=C3)OC2=C1)NC5=CC=C(C(F)(F)F)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

COH-19 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

COH-19COH19COH 19mTORApoptosisMitochondrial MetabolismPI3KAktMammalian target of RapamycinPhosphoinositide 3-kinasePKBProtein kinase BPI3K/Akt/mTOR signaling pathwayMG-63breast cancerMDA-MB-231C6A549MCF-7metastatic cancer cellsmitochondrial (intrinsic) pathwayInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
COH-19
Cat. No.:
HY-184167
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.