Acetylspiramycin
Based on 2 publication(s) in Google Scholar
Acetylspiramycin (Spiramycin B) is an effective oral macrolide antibiotic produced by Streptomyces, It can inhibit the splenic lymphocyte transformation induced by phytohemagglutinin (PHA), LPS (HY-D1056) and antigen, reduce the procoagulant activity of macrophages, have good antibacterial effect on gram-positive bacteria, and is also an effective antigenic insect agent, which can be used to fight parasitic infection.
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- Pureza : 91.60%
- No. CAS: 24916-51-6
- Fòrmula: C45H76N2O15
- Peso molecular:885.09
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Acetylspiramycin
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Actividad biológica
Descripciòn
IC50 & Target
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Macrolide |
In Vitro
Acetylspiramycin (0-40 μg/mL, 0-72 h) can dose-dependently inhibit lymphocyte proliferation in spleen cell culture medium supplemented with plant hemagglutinin (PHA, 10 μg/mL), and the extent of its effect depends on the time of addition[3].
Acetylspiramycin (25-100 μg/ml, 18 h) can reduce the phagocytosis of mouse peritoneal macrophages and has reversible toxicity to macrophages in vitro[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Spleen cells were cultured with PHA (10 μg/mL)
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Concentration:0-40 μg/mL
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Incubation Time:0-72 h
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Result:Inhibited 3HT incorporation in a dose-dependent manner.
Inhibited the proliferation response of B lymphocytes to LPS.
Inhibited antigen-induced proliferation of SRBC-immunized mouse spleen cells.
In Vivo
Acetylspiramycin (50-200 mg/kg/day; Oral gavage (p.o.); 14 days) in CBA/H mouse models can increase the efficiency of phagocytosis in mice[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CBA/H mice
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Dosage:25-200 mg/kg/day
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Administration:Oral gavage (p.o.), 7 days
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Result:Decreased lymphokine activity in a dose-dependent manner
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Animal Model:CBA/H mice[4]
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Dosage:50-200 mg/kg/day
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Administration:Oral gavage (p.o.), 14 days
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Result:Increased the efficiency of phagocytosis in mice.
Chemical Information
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No. CAS 24916-51-6
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Appearance Solid
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Peso molecular 885.09
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Fòrmula C45H76N2O15
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Color White to off-white
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SMILES
O[C@H]1[C@](O[C@H](C)[C@@H](O[C@@](O[C@@H](C)[C@@H]2O)([H])C[C@]2(O)C)[C@@H]1N(C)C)([H])O[C@@H]([C@H](C[C@H]([C@H](/C=C/C=C/C3)O[C@](CC[C@@H]4N(C)C)([H])O[C@@H]4C)C)CC=O)[C@H]([C@](CC(O[C@@H]3C)=O)([H])OC(C)=O)OC
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Synonyms
Spiramycin B; Spiramycin II; Foromacidin B
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Repurposing carrimycin as an antiviral agent against human coronaviruses, including the currently pandemic SARS-CoV-2. [Abstract]2021 Sep;11(9):2850-2858. PMID: 33723501 -
Cell Prolif
Direct inhibitory effect on viral entry of influenza A and SARS-CoV-2 viruses by azithromycin. [Abstract]2021 Jan;54(1):e12953. PMID: 33211371
Solvente y solubilidad
In Vitro:
DMSO : ≥ 50 mg/mL (56.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Pureza y Documentación
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Ficha de datos (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Min-Zhu Huang, et al. Therapeutic effects of acetylspiramycin and garlicin on cryptosporidiosis among drug users. Int J Parasitol Drugs Drug Resist [Content Brief]
[2]. Acetylspiramycin
[3]. Li SY, et al. Acetylspiramycin and the immune system--II. Effects on lymphocyte proliferation, lymphokine production, delayed-type hypersensitivity and antibody production. Int J Immunopharmacol. 1986;8(6):657-664. [Content Brief]
[4]. Li SY, et al. Acetylspiramycin and the immune system. I. Effects of acetylspiramycin on phagocytosis by mouse macrophages in vitro and in vivo. Int J Immunopharmacol. 1985;7(6):881-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1298 mL | 5.6491 mL | 11.2983 mL | 28.2457 mL |
| 5 mM | 0.2260 mL | 1.1298 mL | 2.2597 mL | 5.6491 mL | |
| 10 mM | 0.1130 mL | 0.5649 mL | 1.1298 mL | 2.8246 mL | |
| 15 mM | 0.0753 mL | 0.3766 mL | 0.7532 mL | 1.8830 mL | |
| 20 mM | 0.0565 mL | 0.2825 mL | 0.5649 mL | 1.4123 mL | |
| 25 mM | 0.0452 mL | 0.2260 mL | 0.4519 mL | 1.1298 mL | |
| 30 mM | 0.0377 mL | 0.1883 mL | 0.3766 mL | 0.9415 mL | |
| 40 mM | 0.0282 mL | 0.1412 mL | 0.2825 mL | 0.7061 mL | |
| 50 mM | 0.0226 mL | 0.1130 mL | 0.2260 mL | 0.5649 mL |
Acetylspiramycin Related Classifications
HY-B1916 Related Classifications
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