Cat. No.: HY-17506A

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Azithromycin hydrate

(Synonyms: CP-62993 dihydrate)

45 Cited Publications

Customer Review

Based on 45 publication(s) in Google Scholar

Azithromycin hydrate is a macrolide antibiotic useful for the treatment of a number of bacterial infections.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 117772-70-0
Formula: C₃₈H₇₆N₂O₁₄
Molecular Weight:785.02

Storage:

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Azithromycin hydrate Chemical Structure

CAS No.: 117772-70-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (45)
Solvent & Solubility
- In Vitro
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Azithromycin hydrate is a macrolide antibiotic useful for the treatment of a number of bacterial infections.

IC₅₀ & Target

Macrolide

All Antibiotic Isoforms :

All Parasite Isoforms :

In Vitro

Azithromycin (2 μM) augments rhinovirus-induced IFNβ expression in primary bronchial epithelial cells from asthmatics, which is associated with over-expression of RIG-I like receptors and repression of viral replication. Knockdown of MDA5, but not knockdown of RIG-I, diminishes azithromycin (2 μM)-enhanced viral-induced IFNβ expression in asthmatic primary bronchial epithelial cells^[1]. Azithromycin specifically reduces MMP-9 mRNA and protein levels without affecting NF-κB in endotoxin-challenged monocytic THP-1 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Azithromycin (50 mg/kg) has no effect on bronchoalveolar lavage inflammatory parameters and LDH levels in a mouse model of asthma exacerbation. Azithromycin induces neither general inflammatory parameters nor LDH release in a mouse model of asthma exacerbation, and augments expression of interferon-stimulated genes and the pattern recognition receptor MDA5 but not RIG-I in exacerbating mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 117772-70-0
Appearance Solid
Molecular Weight 785.02
Formula C₃₈H₇₆N₂O₁₄
Color White to off-white
SMILES

C[C@@H]([C@@H]([C@@](C(O[C@@H]([C@@](C)(O)[C@@H]1O)CC)=O)([H])C)O[C@@](O[C@@H](C)[C@@H]2O)([H])C[C@@]2(C)OC)[C@H]([C@](O)(C[C@H](CN([C@@H]1C)C)C)C)O[C@@](O[C@H](C)C[C@@H]3N(C)C)([H])[C@@H]3O.O.O
Synonyms

CP-62993 dihydrate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (45)

All Microbiological AssayRT-PCRWBIn Vivo Efficacy StudyHistological Imaging/Staining

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Journal Impact Factor

Bioact Mater

A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming. [Abstract]

2025 Jun 5:52:73-91. PMID: 40530415
Acta Pharm Sin B

Repurposing carrimycin as an antiviral agent against human coronaviruses, including the currently pandemic SARS-CoV-2. [Abstract]

2021 Sep;11(9):2850-2858. PMID: 33723501

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Sep;11(9):2850-2858. [Abstract]

Antiviral activity of tested macrolide antibiotics against HCoV-229E and HCoV-OC43 in vitro.
Adv Sci (Weinh)

A Macromolecule Reversing Antibiotic Resistance Phenotype and Repurposing Drugs as Potent Antibiotics. [Abstract]

2020 Jul 21;7(17):2001374. PMID: 32995131

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jul 21;7(17):2001374. [Abstract]

Guanidin-functionalized polycarbonate pEt_20 with a degree of polymerization (DP) of 20 was more effective than colistin sulfate in reducing the MIC and MBC of Azithromycin against both strains.
Theranostics

Antibiotic Azithromycin inhibits brown/beige fat functionality and promotes obesity in human and rodents. [Abstract]

2022 Jan 1;12(3):1187-1203. PMID: 35154482

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2022 Jan 1;12(3):1187-1203. [Abstract]

mRNA levels of thermogenic and mitochondrial gene programs in immortal beige adipocytes treated with or without Azithromycin (AZI) at 5 μM for 24 and 48 h.

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2022 Jan 1;12(3):1187-1203. [Abstract]

UCP1 and PGC1α protein levels in immortal beige adipocytes treated with or without Azithromycin (AZI) at 5 μM for 24 and 48 h.

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2022 Jan 1;12(3):1187-1203. [Abstract]

Azithromycin treatment induced adiposity and metabolic dysfunctions in mice for HFD. Metabolic performances (relative changes in body weight and body fat) of HFD fed mice treated with or without Azithromycin via drinking water for 12 weeks. (ABX is given throughout the whole process) (ABX: 1.0 g/L; Azithromycin: 50 mg/kg/day) (n = 6 per group).

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2022 Jan 1;12(3):1187-1203. [Abstract]

Azithromycin treatment induced adiposity and metabolic dysfunctions in mice for HFD. Metabolic performances (relative glucose tolerance test (GTT) and area under the curve (AUC) analysis) of HFD fed mice treated with or without Azithromycin via drinking water for 12 weeks. (ABX is given throughout the whole process) (ABX: 1.0 g/L; Azithromycin: 50 mg/kg/day) (n = 6 per group).

Azithromycin hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2022 Jan 1;12(3):1187-1203. [Abstract]

Azithromycin treatment suppressed energy expenditure and thermogenic capacity in mice for HFD. Metabolic performances of HFD fed mice treated with or without Azithromycin via drinking water for 12 weeks (ABX is given throughout the whole process) (ABX: 1.0 g/L; Azithromycin: 50 mg/kg/day) (n = 6). Representative images of H&E staining of fat tissues.
Mol Ther

Nucleic acid aptamer controls mycoplasma infection for inhibiting the malignancy of esophageal squamous cell carcinoma. [Abstract]

2022 Jun 1;30(6):2224-2241. PMID: 35189346
Nano Today

2025 Apr.
J Exp Med

Antibiotic use in early life impairs MAIT cell-mediated immunity in adulthood. [Abstract]

2026 Mar 2;223(3):e20241287. PMID: 41400657
Cell Rep Med

Vesicle-mediated mitochondrial clearance presents an actionable metabolic vulnerability in triple-negative breast cancer. [Abstract]

2025 Dec 16;6(12):102478. PMID: 41406946
Emerg Microbes Infect

AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. [Abstract]

2024 Dec;13(1):2321981. PMID: 38422452
Cell Rep

Intratumor Mycoplasma promotes the initiation and progression of hepatocellular carcinoma. [Abstract]

2023 Dec 12;42(12):113563. PMID: 38088929
Biochem Pharmacol

Azithromycin modulates Teff/Treg balance in retinal inflammation via the mTOR signaling pathway. [Abstract]

2021 Nov:193:114793. PMID: 34600916
PLoS Pathog

Suppressing neutrophil itaconate production attenuates Mycoplasma pneumoniae pneumonia. [Abstract]

2024 Nov 5;20(11):e1012614. PMID: 39499730
Invest Ophthalmol Vis Sci

Hedgehog Signaling Pathway Regulates the Proliferation and Differentiation of Rat Meibomian Gland Epithelial Cells. [Abstract]

2021 Feb 1;62(2):33. PMID: 33616621
Antimicrob Agents Chemother

Stepwise evolution and clonal enrichment of gepotidacin resistance in Neisseria gonorrhoeae. [Abstract]

2026 Apr;70(4):e0170625. PMID: 41810969
mLife

Identification of a phenyl ester covalent inhibitor of caseinolytic protease and analysis of the ClpP1P2 inhibition in mycobacteria. [Abstract]

2025 Apr 15;4(2):155-168. PMID: 40313980
ACS Omega

Lapatinib Acts against Biofilm Formation and the Hemolytic Activity of Staphylococcus aureus. [Abstract]

2022 Mar 3;7(10):9004-9014. PMID: 35309438
iScience

Loratadine inhibits Staphylococcus aureus virulence and biofilm formation. [Abstract]

2022 Jan 5;25(2):103731. PMID: 35098100
Microbiol Spectr

Montelukast treats Streptococcus pneumoniae-induced sepsis via antibacterial and anti-inflammatory activities. [Abstract]

2025 Oct 15:e0122125. PMID: 41090944
Microbiol Spectr

Killing of colistin-resistant Aeromonas hydrophila by a synthetic peptide. [Abstract]

2025 Aug 29:e0059025. PMID: 40879403
Microbiol Spectr

Antimicrobial resistance of rapidly growing mycobacteria isolated from companion animals in Taiwan. [Abstract]

2025 May 19:e0307424. PMID: 40387379
ACS Infect Dis

Lusutrombopag as a Repurposing Drug in Combination with Aminoglycosides against Vancomycin-Resistant Enterococcus. [Abstract]

2024 Apr 12;10(4):1327-1338. PMID: 38567846
ACS Chem Biol

Investigate Natural Product Indolmycin and the Synthetically Improved Analogue Toward Antimycobacterial Agents. [Abstract]

2022 Jan 21;17(1):39-53. PMID: 34908399
Viruses

Broad-Spectrum Host-Based Antivirals Targeting the Interferon and Lipogenesis Pathways as Potential Treatment Options for the Pandemic Coronavirus Disease 2019 (COVID-19). [Abstract]

2020 Jun 10;12(6):628. PMID: 32532085
Viruses

Erythromycin Estolate Inhibits Zika Virus Infection by Blocking Viral Entry as a Viral Inactivator. [Abstract]

2019 Nov 15;11(11):1064. PMID: 31731598
Pathogens

2026 Feb;15(2):169.
Pathogens

N-Acetylcysteine as a Host-Directed Therapy Against Clarithromycin-Resistant Mycobacterium abscessus. [Abstract]

2025 Mar 21;14(4):302. PMID: 40333083
Pathogens

Mycoplasma bovis Invades Non-Phagocytic Cells by Clathrin-Dependent Endocytic Pathways and Escapes from Phagocytic Vesicles. [Abstract]

2024 Nov 15;13(11):1003. PMID: 39599557
Diabetes Metab Syndr Obes

A Network Pharmacology-Based Investigation into the Mechanism of Quercetin Combined with Rosuvastatin in Delaying Diabetic Nephropathy via Inhibiting NRK-52E Cell Ferroptosis. [Abstract]

2025 Aug 5:18:2681-2694. PMID: 40785752
Water

2025 Jun 6.
Vet Microbiol

Emergence of multidrug resistance and genomic analysis of azithromycin-resistant non-typhoidal Salmonella from diverse sources in China, 2006-2018. [Abstract]

2025 Jun 4:307:110582. PMID: 40482360
Immunol Invest

The Mechanism of Baicalin in the Treatment of Mycoplasma Pneumoniae Pneumonia by Regulating NLRP3/Caspase-1 Signaling Pathway. [Abstract]

2025 Jan 9:1-13. PMID: 39781905
Immunobiology

Dual-phase study of CD4+CXCR4+ T cells in Mycoplasma pneumoniae pneumonia: clinical correlations in children and therapeutic exploration with tofacitinib in mice. [Abstract]

2025 Nov;230(6):153136. PMID: 41192098
Biochem Biophys Res Commun

Combinatorial screening of a panel of FDA-approved drugs identifies several candidates with anti-Ebola activities. [Abstract]

2020 Feb 19;522(4):862-868. PMID: 31806372
Rapid Commun Mass Spectrom

Sensitive Detection of Salmonella Susceptibility to Ceftriaxone and Azithromycin Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. [Abstract]

2026 May 30;40(10):e70053. PMID: 41704149
COVID

2021, 1(1), 263-275.
bioRxiv

2025 Jun 11.
Res Sq

2025 Apr 16.
bioRxiv

2024 May 10.
bioRxiv

2024 Apr 4.
Research Square Preprint

2022 Jun.
Journal of Pharmaceutical Research International

33(47B): 274-279, 2021.
bioRxiv

2021 Jan 19.
Universidade de São Paulo

2020 Sep.
Charles University

2020 Jul.
Patent

US20200101105A1.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (127.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2739 mL

6.3693 mL

12.7385 mL

5 mM

0.2548 mL

1.2739 mL

2.5477 mL

10 mM

0.1274 mL

0.6369 mL

1.2739 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Protocol

Cell Assay^[2]

THP-1 cells (10⁶ cells in 1 mL RPMI medium, without antibiotics, growth factors or serum) are seeded in each well of 24-well plates and allowed to settle for 1 hour. Next, 50 μL of the test compound is added followed by 50 μL of LPS (final concentration of 10 μg/mL). After 24h (37°C and 5% CO₂) the supernatants and cell pellets are collected (1200 rpm, 5 min). THP-1 cell viability is tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). MTT is dissolved at 2 mg/mL in PBS and aliquots are stored at -20°C. The MTT assay is performed according to the suppliers instructions. Absorbance of MTT converted into formazan is measured at a wavelength of 570 nm with background subtraction at 630 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Menzel M, et al. Azithromycin augments rhinovirus-induced IFNβ via cytosolic MDA5 in experimental models of asthma exacerbation. Oncotarget. 2017 Mar 18. [Content Brief]

[2]. Vandooren J, et al. Differential inhibition of activity, activation and gene expression of MMP-9 in THP-1 cells by azithromycin and minocycline versus bortezomib: A comparative study. PLoS One. 2017 Apr 3;12(4):e0174853. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2739 mL	6.3693 mL	12.7385 mL	31.8463 mL
	5 mM	0.2548 mL	1.2739 mL	2.5477 mL	6.3693 mL
	10 mM	0.1274 mL	0.6369 mL	1.2739 mL	3.1846 mL
	15 mM	0.0849 mL	0.4246 mL	0.8492 mL	2.1231 mL
	20 mM	0.0637 mL	0.3185 mL	0.6369 mL	1.5923 mL
	25 mM	0.0510 mL	0.2548 mL	0.5095 mL	1.2739 mL
	30 mM	0.0425 mL	0.2123 mL	0.4246 mL	1.0615 mL
	40 mM	0.0318 mL	0.1592 mL	0.3185 mL	0.7962 mL
	50 mM	0.0255 mL	0.1274 mL	0.2548 mL	0.6369 mL
	60 mM	0.0212 mL	0.1062 mL	0.2123 mL	0.5308 mL
	80 mM	0.0159 mL	0.0796 mL	0.1592 mL	0.3981 mL
	100 mM	0.0127 mL	0.0637 mL	0.1274 mL	0.3185 mL

Keywords

Azithromycin hydrate

45 Cited Publications

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Azithromycin hydrate

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Publications Citing Use of MedChemExpress (MCE) Azithromycin hydrate

All Antibiotic Isoforms

All Parasite Isoforms

Biological Activity

Chemical Information

Publications (45)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Azithromycin hydrate Related Classifications

Cat. No.: HY-17506A