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Cat. No.: HY-190477

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WC-27

WC-27 is a selective Wee1 inhibitor with an IC₅₀ of 2.11 nM. WC-27 blocks Wee1-mediated inhibitory phosphorylation of CDK1 and disrupts the G2/M checkpoint. WC-27 induces S-phase cell cycle arrest and apoptosis, and inhibits cell migration and colony formation. WC-27 can be used for research on non-small cell lung cancer.

For research use only. We do not sell to patients.

CAS No.: 3069940-08-2
Formula: C₂₂H₂₅Cl₂N₇O₂
Molecular Weight:490.39

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

WC-27 Chemical Structure

CAS No.: 3069940-08-2

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Parmacokinetics
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

WEE1

2.11 nM (IC₅₀)

CDK1

All Wee1 Isoforms :

PKMYT1

All CDK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
NCI-H1299	IC50	1.05 μM	Antiproliferative activity against human NCI-H1299 non-small cell lung cancer cells assessed as viability reduction incubated 72 hrs by MTT assay. Antiproliferative activity against human NCI-H1299 non-small cell lung cancer cells assessed as viability reduction incubated 72 hrs by MTT assay.	42887067
LoVo	IC50	0.38 μM	Antiproliferative activity against human LOVO cells assessed as viability reduction incubated 72 hrs by MTT assay. Antiproliferative activity against human LOVO cells assessed as viability reduction incubated 72 hrs by MTT assay.	42887067
HCC1569	IC50	0.79 μM	Antiproliferative activity against human HCC1569 cells assessed as viability reduction incubated 72 hrs by MTT assay. Antiproliferative activity against human HCC1569 cells assessed as viability reduction incubated 72 hrs by MTT assay.	42887067

In Vitro

Anti-cancerCell Cycle Regulation

Anti-cancer

WC-27 (72 h) inhibits NCI-H1299 cell proliferation with an IC₅₀ of 1.05 µM^[1].
WC-27 (72 h) inhibits LOVO and HCC1569 cell proliferation with IC₅₀ values of 0.38 µM and 0.79 µM, respectively^[1].
WC-27 (2.5 μM) inhibits phosphorylation of CDK1 at Tyr15 in NCI-H1299 cells^[1].
WC-27 (0.625-5.0 μM; 24 h) inhibits the migration of NCI-H1299 cells in a concentration-dependent manner^[1].
WC-27 (0.625-1.25 μM; 10 days) inhibits colony formation of NCI-H1299 cells in a concentration-dependent manner^[1].

Anti-cancer

Cell Cycle Regulation

WC-27 (0.625-5.0 μM; 72 h) induces S-phase arrest in NCI-H1299 cells in a concentration-dependent manner^[1].

Cell Cycle Regulation

WC-27 is a potent Wee1 inhibitor with an IC₅₀ of 2.11 nM^[1] in the cell-free Wee1 enzymatic assay.
WC-27 potently inhibits Wee1 in a 100-kinase panel (97.22% inhibition at 500 nM), while showing weak inhibition of PLK1 and several cell cycle kinases^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:

NCI-H1299 cells
Concentration:

0.625, 1.25, 2.5, and 5.0 µM
Incubation Time:

72 h
Result:

S-phase cells: 15.24% at 0.625 µM.
S-phase cells: 21.39% at 1.25 µM.
S-phase cells: 31.39% at 2.5 µM.
S-phase cells: 51.93% at 5.0 µM.

Cell Migration Assay ^[1]

Cell Line:

NCI-H1299 cells
Concentration:

0.625-5.0 µM
Incubation Time:

24 h
Result:

Significantly inhibited wound closure of NCI-H1299 cells in a concentration-dependent manner.
Wound healing rate markedly decreased as concentration increased from 0.625 µM to 5.0 µM.

Cell Proliferation Assay^[1]

Cell Line:

NCI-H1299 cells
Concentration:

0.625 and 1.25 µM
Incubation Time:

10 days
Result:

Colony formation rates decreased significantly.
Near-complete inhibition of colony formation was observed at 0.625 µM.
Almost no colonies formed at 1.25 µM.

Related Protocols :

Parmacokinetics

Species	Dose	Route	AUC_0-t	T_1/2	Clearance (CL)	MRT_0-last	V_dss	C_max	T_max	F
Rat^[1]	10 mg/kg	i.g.	2370 ng·h/mL	3.13 h	/	6.05 h	/	208 ng/mL	4.00 h	49.5 %
Rat^[1]	1 mg/kg	i.v.	412 ng·h/mL	3.07 h	35.2 mL/min/kg	2.35 h	7.93 L/kg	/	/	/

Chemical Information

CAS No. 3069940-08-2
Molecular Weight 490.39
Formula C₂₂H₂₅Cl₂N₇O₂
SMILES

O=C(NC1=C(Cl)C=CC=C1Cl)C2=CN=C(NC3=CN(C4CCN(C)CC4)N=C3)N=C2OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Chen F, et al. Design, synthesis, and biological evaluation of 2-aminopyrimidine-5-carboxamide derivatives as potent selective Wee1 inhibitors. European journal of medicinal chemistry. 2026 Oct 07;321(Pt 2):119402.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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WC-27

All Wee1 Isoforms

All CDK Isoforms