Equisetin
Based on 1 Customer Validation
Equisetin is an N-methylserine-derived acyl tetramic acid, quorum sensing inhibitor (QSI), herbicides and antibiotics. Equisetin specifically inhibits the anionic carriers of substrates in the inner mitochondrial membrane. Equisetin inhibits the activity of HIV-1 integrase, 11β-HSD1, and 2,4-dinitrophenol (Dnp)-stimulated ATPase (IC50 = ~8 nmol per mg of protein). Equisetin exhibits growth inhibition of bacteria, anti-inflammatory, amelioration of lipid-associated disorders, and cytotoxic effects.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.40%
- CAS 番号: 57749-43-6
- 分子式: C22H31NO4
- 分子量:373.49
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
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STAT3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | GI50 |
144 nM
Compound: 5, NSC 772378
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Cytotoxicity against human CCRF-CEM cells assessed as growth inhibition by SRB assay
Cytotoxicity against human CCRF-CEM cells assessed as growth inhibition by SRB assay
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[PMID: 24422636] |
Equisetin (50-300 μM, 6 h) inhibit the production of virulence factor, biofilm formation, and swarming motility of Pseudomonas aeruginosa PAO1[4].
Equisetin shows cytotoxicity against human CCRF-CEM cells assessed as growth inhibition by SRB assay, with a GI50 of 144 nM[7].
Equisetin (1-6 μg/mL, 0-6 h) removes Staphylococcus aureus from IEC-6 cells by enhancing host autophagy and inducing mitochondria-mediated ROS generation[8].
Equisetin (20 μM, 7 days) inhibits adiposity through AMPK-dependent regulation of brown adipocyte (BA) differentiation[9].
Equisetin (0.01-100 μM, pre-stimulates 12 h) attenuates lipid droplet accumulation and foam cell formation in macrophages[10].
Equisetin (0.01-10 μM) inhibits LPS and oxLDL-induced inflammatory responses in macrophages (RAW264.7 and BMDM)[10].
Equisetin (4 µg/mL) can potentiate Colistin (HY-113678) activity against multi-drug resistant gram-negative bacteria including Colistin -resistant strains[11].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:3T3-L1
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Concentration:20 μM
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Incubation Time:7 days
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Result:Increased the specific BA markers Prdm16 and the uncoupling protein Ucp1, the mitochondrial markers Pgc1α, and Tfam.
Increased AMPK and phosphorylated AMPK (pAMPK).
Equisetin (10 mg/kg, i.p., 6 h) shows significant anti-infective activity in a mouse model of Staphylococcus aureus ATCC 29213 infection[8].
Equisetin (5-20 mg/kg, p.o., every two days until 12 weeks) prevents atherosclerosis in mice by binding to and inhibiting the activity of STAT3[10].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Atherosclerosis model (eight-week-old male ApoE-/- mice were fed with HFD for 12 weeks)[10]
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Dosage:5, 20 mg/kg
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Administration:Oral gavage (p.o.), every two days until 12 weeks
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Result:Attenuated HFD-induced lipid area in the en face prepared aorta and aortic root.
Attenuated atherosclerotic lesion area (HFD versus HFD+5 mg/kg Equisetin: 31.71 versus 16.95; HFD versus HFD+20 mg/kg Equisetin: 31.71 versus 10.12).
Increased collagen content in the aortic plaques.
Increased α-SMA-positive areas in the plaques in aortic root.
化学情報
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CAS 番号 57749-43-6
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性状 Solid
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分子量 373.49
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分子式 C22H31NO4
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Color White to off-white
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SMILES
O=C(/C1=C(O)\[C@]2(C)[C@H](/C=C/C)C=C[C@]3([H])C[C@H](C)CC[C@@]23[H])N(C)[C@@H](CO)C1=O
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Structure Classification
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Initial Source
marine fungus Fusarium sp. Z10
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 1 mg/mL (2.68 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (287 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Burmeister HR, et al. Antibiotic produced by Fusarium equiseti NRRL 5537. Antimicrob Agents Chemother. 1974 Jun;5(6):634-9. [Content Brief]
[2]. Vesonder RF, et al. Equisetin, an antibiotic from Fusarium equiseti NRRL 5537, identified as a derivative of N-methyl-2, 4-pyrollidone. J Antibiot (Tokyo). 1979 Jul;32(7):759-61. [Content Brief]
[3]. Lee J, et al. The hierarchy quorum sensing network in Pseudomonas aeruginosa. Protein Cell. 2015 Jan;6(1):26-41. [Content Brief]
[4]. Zhang M, et al. Equisetin as potential quorum sensing inhibitor of Pseudomonas aeruginosa. Biotechnol Lett. 2018 May;40(5):865-870. [Content Brief]
[5]. Xu Z, et al. Equisetin is an anti-obesity candidate through targeting 11β-HSD1[J]. Acta Pharmaceutica Sinica B, 2022, 12(5): 2358-2373. [Content Brief]
[6]. König T, Kapus A, Sarkadi B. Effects of equisetin on rat liver mitochondria: evidence for inhibition of substrate anion carriers of the inner membrane. J Bioenerg Biomembr. 1993 Oct;25(5):537-45. [Content Brief]
[7]. Whitt J, et al. Tetramic acid analogues produced by coculture of Saccharopolyspora erythraea with Fusarium pallidoroseum. J Nat Prod. 2014 Jan 24;77(1):173-7. [Content Brief]
[8]. Tian J, et al. Equisetin Targets Intracellular Staphylococcus aureus through a Host Acting Strategy. Mar Drugs. 2022 Oct 22;20(11):656. [Content Brief]
[9]. Zhong Q, et al.Equisetin inhibits adiposity through AMPK-dependent regulation of brown adipocyte differentiation. Heliyon. 2024 Feb 1;10(3):e25458. [Content Brief]
[10]. Yang Y, et al. Equisetin protects from atherosclerosis in vivo by binding to STAT3 and inhibiting its activity. Pharmacol Res. 2024 Aug;206:107289. [Content Brief]
[11]. Zhang Q, et al. Equisetin Restores Colistin Sensitivity against Multi-Drug Resistant Gram-Negative Bacteria. Antibiotics (Basel). 2021 Oct 18;10(10):1263. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6774 mL | 13.3872 mL | 26.7745 mL | 66.9362 mL |
Equisetin 関連分類
HY-N6711 関連分類
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