Cat. No.: HY-N2359

Chelerythrine

19 Cited Publications

고객리뷰

Based on 19 publication(s) in Google Scholar

Chelerythrine is a natural alkaloid, acts as a potent and selective Ca²⁺/phospholopid-dependent PKC antagonist, with an IC₅₀ of 0.7 μM. Chelerythrine has antitumor, antidiabetic and anti-inflammatory activity. Chelerythrine inhibits the BclXL-Bak BH3 peptide binding with IC₅₀ of 1.5 μM and displaces Bax from BclXL. Chelerythrine triggers apoptosis and autophagy.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No.: 34316-15-9
화학식: C₂₁H₁₈NO₄
분자량:348.37

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (277 KB)

Chelerythrine Chemical Structure

CAS No.: 34316-15-9

사이즈

가격

재고

수량

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (19)
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

Chelerythrine is a natural alkaloid, acts as a potent and selective Ca²⁺/phospholopid-dependent PKC antagonist, with an IC₅₀ of 0.7 μM^[1]. Chelerythrine has antitumor, antidiabetic and anti-inflammatory activity^[2]. Chelerythrine inhibits the BclXL-Bak BH3 peptide binding with IC₅₀ of 1.5 μM and displaces Bax from BclXL. Chelerythrine triggers apoptosis and autophagy^[3]^[4].

IC₅₀ & Target^[1]

PKC

0.7 μM (IC₅₀)

All Beta-lactamase Isoforms :

All PKC Isoforms :

All Bcl-2 Family Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780/Taxol	IC₅₀	0.4 μM Compound: 5	Antiproliferative activity against human A2780T cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human A2780T cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay	[PMID: 39213483]
A549	IC₅₀	7.73 μM Compound: Chelerythrine	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27561984]
A549/TR	IC₅₀	0.4 μM Compound: 5	Antiproliferative activity against human A549/Taxol cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human A549/Taxol cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay	[PMID: 39213483]
HUVEC	IC₅₀	4.93 μM Compound: Chelerythrine	Cytotoxicity against HUVEC assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against HUVEC assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27561984]
LoVo	IC₅₀	0.4 μM Compound: 5	Antiproliferative activity against human LoVo cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human LoVo cells assessed as reduction in cell viability incubated for 48 hrs by CCK-8 assay	[PMID: 39213483]
MKN-45	IC₅₀	12.7 μM Compound: Chelerythrine	Cytotoxicity against human MKN45 cells assessed as decrease in cell viability after 48 hrs by MTT assay Cytotoxicity against human MKN45 cells assessed as decrease in cell viability after 48 hrs by MTT assay	[PMID: 27887841]
NB-4	IC₅₀	1.49 μM Compound: Chelerythrine	Cytotoxicity against human NB4 cells assessed as decrease in cell viability after 48 hrs by MTT assay Cytotoxicity against human NB4 cells assessed as decrease in cell viability after 48 hrs by MTT assay	[PMID: 27887841]
NCI-H1299	IC₅₀	4.28 μM Compound: Chelerythrine	Cytotoxicity against human NCI-H1299 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human NCI-H1299 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27561984]
NCI-H292	IC₅₀	4.96 μM Compound: Chelerythrine	Cytotoxicity against human NCI-H292 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human NCI-H292 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27561984]
NCI-H460	IC₅₀	5.14 μM Compound: Chelerythrine	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27561984]
PC-3	IC₅₀	0.4 μM Compound: 5	Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay	[PMID: 39213483]

In Vitro

Chelerythrine (48 h) inhibits the growth of L-1210 cells (IC₅₀: 0.53 uM )^[1].
Chelerythrine (0-20 μM, 24 h) inhibits cell viability, induces apoptosis and autophagy in A549 and NCI-H1299 cells^[4].
Chelerythrine (0-5 μM, 24 or 48 h) induces apoptosis in BclXL-overexpressing SH-SY5Y cells^[3].
Chelerythrine (2.5-10 μM, 16 h) induces mitochondrial depolarization (decrease in mitochondrial potential) in SH-SY5Y cells, and stimulates release of CytC from isolated mitochondria^[4].
Chelerythrine (0-100 ng/mL, 24 h) reduces the LPS induced production of NO and TNF-α in primary macrophages^[5].
Chelerythrine (MIC: 0.156 mg/mL) shows antibacterial activities against Gram-positive bacteria, Staphylococcus aureus (SA), MRSA, and extended spectrum β-lactamase S. aureus (ESBLs-SA)^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

A549 and NCI-H1299 cells
Concentration:

10, 15, 20 μM
Incubation Time:

24 h
Result:

Induced expression of LC3-II in a beclin 1-dependent way.

In Vivo

Chelerythrine (5 mg/kg, i.p., daily) attenuates partial unilateral ureteral obstruction (UUO) induced kidney injury, and restores renal function in neonatal rats^[2].
Chelerythrine (1-10 mg/kg, i.p., at 24 and 1 h before injection of 100 μg/kg LPS) shows anti-inflammatory effects (increased survival rate, decreased serum nitrite and TNF-α level) in LPS induced mice endotoxic shock model^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Unilateral ureteral obstruction (UUO) induced neonatal rats^[2]
Dosage:

5 mg/kg
Administration:

i.p., daily
Result:

Attenuated kidney injury (Increased kidney weight and restored renal function).
Inhibited UUO-induced upregulated kidney injury molecule-1 expression, apoptosis, and renal fibrosis.

Chemical Information

CAS No. 34316-15-9
분자량 348.37
화학식 C₂₁H₁₈NO₄
SMILES

C[N+]1=CC2=C(OC)C(OC)=CC=C2C(C=CC3=C4)=C1C3=CC5=C4OCO5
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (19)

All WBHistological Imaging/Staining

Sort by:

Journal Impact Factor

Cell Commun Signal

Zinc-mediated activation of CREB pathway in proliferation of pulmonary artery smooth muscle cells in pulmonary hypertension. [Abstract]

2021 Oct 11;19(1):103. PMID: 34635097
J Headache Pain

Calcitonin gene-related peptide receptor antagonist BIBN4096BS regulates synaptic transmission in the vestibular nucleus and improves vestibular function via PKC/ERK/CREB pathway in an experimental chronic migraine rat model. [Abstract]

2022 Mar 8;23(1):35. PMID: 35260079
Phytother Res

Chelerythrine chloride inhibits the progression of colorectal cancer by targeting cancer-associated fibroblasts through intervention with WNT10B/β-catenin and TGFβ2/Smad2/3 axis. [Abstract]

2023 Oct;37(10):4674-4689. PMID: 37402476
J Pineal Res

Melatonin Enhances the Myometrial Contraction Through MT2-PKC-NRF2/MAFF Signaling Pathway in Sows. [Abstract]

2026 Mar;78(2):e70135. PMID: 41854048
Cancer Cell Int

High FAAP24 expression reveals poor prognosis and an immunosuppressive microenvironment shaping in AML. [Abstract]

2023 Jun 17;23(1):117. PMID: 37328842
Nutrients

Dietary Copper on the Onset of Puberty in Rats: Possible Mechanism. [Abstract]

2025 Nov 12;17(22):3534. PMID: 41305585

Chelerythrine purchased from MedChemExpress. Usage Cited in: Nutrients. 2025 Nov 12;17(22):3534. [Abstract]

The PKC protein expression in the Chelerythrine (0-8μM)-treated groups gradually decreased with increasing dose.
Front Pharmacol

Propofol Inhibits Ischemia/Reperfusion-Induced Cardiotoxicity Through the Protein Kinase C/Nuclear Factor Erythroid 2-Related Factor Pathway. [Abstract]

2021 May 13:12:655726. PMID: 34054535
Sci Rep

Neuroprotective Effects of CGP3466B on Apoptosis Are Modulated by Protein-L-isoaspartate (D-aspartate) O-methyltransferase/Mst1 Pathways after Traumatic Brain Injury in Rats. [Abstract]

2017 Aug 23;7(1):9201. PMID: 28835703

Chelerythrine purchased from MedChemExpress. Usage Cited in: Sci Rep. 2017 Aug 23;7(1):9201. [Abstract]

CGP3466B upregulates PCMT1 expression and inhibits cleaved-Mst1 activation at 24 h after TBI. Chelerythrine activates the Mst1 without regulating PCMT1 protein level. (a) Western blot assay for the expression of PCMT1. (b) Western blot assay for the expression of Mst1 and cleaved-Mst1.
J Inflamm Res

FLI1 Induces Plaque Psoriasis and Its Inhibition Attenuates Disease Progression. [Abstract]

2025 Mar 20:18:4213-4231. PMID: 40129872

Chelerythrine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Mar 20:18:4213-4231. [Abstract]

CLT (Chelerythrine chloride, 5 and 8 μM) downregulated the expression of FLI1 in HEL cells, as determined via Western blotting.

Chelerythrine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Mar 20:18:4213-4231. [Abstract]

The effect of Chelerythrine (CLT, 5 and 8 μM) on FLI1 downregulation was significantly diminished in the scrambled control cells versus FLI1 knockdown HEL cells.

Chelerythrine purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2025 Mar 20:18:4213-4231. [Abstract]

IMQ administration. Images in the top showed significant attenuation in IMQ-induced psoriasis following CLT (Chelerythrine chloride, 4 mg/kg) treatment. H&E staining analysis of the dorsal skin of the IMQ group on day 7 revealed significant epidermal hyperplasia, increased acanthosis, parakeratosis, telangiectasia, and inflammatory cell infiltration. CLT injection significantly reversed these histological changes caused by IMQ, showing less parakeratosis and less epidermal thickening than the IMQ group.
Front Physiol

Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs. [Abstract]

2021 Oct 28;12:770430. PMID: 34777026
FASEB J

EphrinB2/ephB2-mediated myenteric synaptic plasticity: mechanisms underlying the persistent muscle hypercontractility and pain in postinfectious IBS. [Abstract]

2019 Dec;33(12):13644-13659. PMID: 31601124
Cytokine

Chelerythrine ameliorates Aspergillus fumigatus keratitis through suppressing the LOX-1/p38 MAPK signaling pathway. [Abstract]

2024 Oct:182:156717. PMID: 39067394
Fishes

2022, 7(5), 229.
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biochem Biophys Res Commun

Chelerythrine inhibits NR2B NMDA receptor independent of PKC activity. [Abstract]

2024 Dec 20:739:150914. PMID: 39536412
Planta Med

Novel Approaches for the Analysis and Isolation of Benzylisoquinoline Alkaloids in Chelidonium majus. [Abstract]

2024 Jun;90(7-08):523-533. PMID: 38843792
Bull Sib Med

2025.
Heliyon

Pan-cancer investigation of RFX family and associated genes identifies RFX8 as a therapeutic target in leukemia. [Abstract]

2024 Jul 28;10(15):e35368. PMID: 39170430
Research Square Preprint

2020 Oct.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Herbert JM, et al. Chelerythrine is a potent and specific inhibitor of protein kinase C. Biochem Biophys Res Commun. 1990 Nov 15;172(3):993-9. [Content Brief]

[2]. Shi B, et al. Protein kinase C inhibitor chelerythrine attenuates partial unilateral ureteral obstruction induced kidney injury in neonatal rats. Life Sci. 2019 Jan 1;216:85-91. [Content Brief]

[3]. Chan SL, et al.Identification of chelerythrine as an inhibitor of BclXL function.J Biol Chem. 2003 Jun 6;278(23):20453-6. [Content Brief]

[4]. Tang ZH, et al.Induction of reactive oxygen species-stimulated distinctive autophagy by chelerythrine in non-small cell lung cancer cells.Redox Biol. 2017 Aug;12:367-376. [Content Brief]

[5]. Li W, et al. Effect of chelerythrine against endotoxic shock in mice and its modulation of inflammatory mediators in peritoneal macrophages through the modulation of mitogen-activated protein kinase (MAPK) pathway. Inflammation. 2012 Dec;35(6):1814-24. [Content Brief]

[6]. He N, et al. Antibacterial mechanism of chelerythrine isolated from root of Toddalia asiatica (Linn) Lam. BMC Complement Altern Med. 2018 Sep 26;18(1):261. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Chelerythrine

19 Cited Publications

100 mg 견적 받기

Subscribe to the

MedChemExpress

Newsletter

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

Chelerythrine

Publications Citing Use of MedChemExpress (MCE) Chelerythrine

All Beta-lactamase Isoforms

All PKC Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (19)

순도&문서

References

Calculators

Help & FAQs

Chelerythrine Related Classifications

HY-N2359 Related Classifications