Nogo-B-IN-1
Nogo-B-IN-1 ((S,R)-4v) is a covalent Nogo-B inhibitor. Nogo-B-IN-1 inhibits the progression of osteosarcoma (OS) by suppressing Nogo-B activity and the PI3K/AKT/NF-κB/Bcl-2 signaling pathway. Nogo-B-IN-1 inhibits the proliferation of OS 143B cells (IC50 = 0.28 µM) and induces apoptosis. Nogo-B-IN-1 can be used for OS targeting research.
For research use only. We do not sell to patients.
- Formula: C29H27Cl3N2O6
- Molecular Weight:605.89
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Nogo-B-IN-1 (0-3 μM, 24 h) inhibits the migration and proliferation, induces abnormal cell morphology, cellular shrinkage, disordered arrangement, apoptosis depends on Nogo-B in 143B cells[1].
Nogo-B-IN-1 (50 μM) inhibits Nogo-B expression, with inhibitory activity of 99% in 143B cells[1].
Nogo-B-IN-1 (0-3 μM, 24 h) can blunt Nogo-B, which in turn impairs PI3K/AKT and NF-κB activities in 143B cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:143B cells
-
Concentration:0 nM, 300 nM, 1000 nM
-
Incubation Time:24 h
-
Result:Decreased the percentage of intercellular wound healing distance, decreased the colony growth.
-
Cell Line:143B cells
-
Concentration:0 nM, 300 nM, 1000 nM
-
Incubation Time:24 h
-
Result:Induced apoptosis.
-
Cell Line:143B cells
-
Concentration:0, 0.3, 0.5, 1, 3 μM
-
Incubation Time:0, 24, 48, 72 h
-
Result:Downregulated Nogo-B protein level, enhanced the stability of Nogo-B.
Decreased p65, Bcl-2 protein, inhibited activation of PI3K, AKT, and p-AKT.
-
Cell Line:143B cells
-
Concentration:0, 0.3, 0.5, 1, 3 μM
-
Incubation Time:24 h
-
Result:Had no effect of Nogo-B mRNA level.
Nogo-B-IN-1 (30 mg/kg, i.p., once a day, 23 days) is weakened the inhibitory effect of tumor volume and tumor weight in 43B with Nogo-B knockdown xenograft BALB/c nude mice model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:143B (5 ×106) xenograft BALB/c nude mice (3-month-old) model[1]
-
Dosage:30 mg/kg
-
Administration:i.p., once a day, 23 days
-
Result:Suppressed tumor growth, had no discernible morphological changes in the heart, liver, spleen, or kidney tissues.
-
Animal Model:143B (5 ×106) with Nogo-B knockdown xenograft BALB/c nude mice (3-month-old) model[1]
-
Dosage:30 mg/kg
-
Administration:i.p., once a day, 23 days
-
Result:Was weakened the inhibitory effect of tumor volume and tumor weight.
Chemical Information
-
Molecular Weight 605.89
-
Formula C29H27Cl3N2O6
-
SMILES
O=C(OC)[C@@](C1=CC=C(Cl)C=C1Cl)(OCC2=CC=CC=C2Cl)[C@@H](NC3=CC=C(NC(C=C)=O)C=C3)C(OCC)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Nogo-B-IN-1 Related Classifications
HY-175317 Related Classifications
Powered by Bioz