Cat. No.: HY-126307

Urolithin B

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Urolithin B is one of Ellagitannins' slow microbial products, and has anti-inflammatory and anti-inflammatory effects. Urolithin B suppresses NF-κB activity. Urolithin B suppresses JNK, ERK and Akt's oxidation, and increases AMPK's oxidation. Urolithin B is also a quantitative change factor for bone and skin quality.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 1139-83-9
Formula: C₁₃H₈O₃
Molecular Weight:212.20

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Urolithin B Chemical Structure

CAS No.: 1139-83-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All NF-κB Isoforms :

All JNK Isoforms :

All ERK Isoforms :

All Akt Isoforms :

All AMPK Isoforms :

All Endogenous Metabolite Isoforms :

In Vitro

Urolithin B (30-100 μM; 24 h) shows anti-inflammatory effects in LPS-stimulated BV2 microglia by modulating pro- and anti-inflammatory molecule. Such like that suppressing NF-κB and AP-1 signaling in LPS-stimulated BV2 cells, and also upregulating AMPK and downregulating the Akt, JNK, and ERK signaling pathway^[1].
Urolithin B (15 μM; 24 h) enhances the growth and differentiation of C2C12 muscle ducts by increasing protein synthesis and inhibiting the ubiquitin-proteasome pathway^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Urolithin B (50 mg/kg; ip; once daily for 4 days) inhibits microglial activation in LPS-injected mouse brains, under neuroinflammatory condition^[1].
Urolithin B (10 μg/day; mini-osmotic pump delivery, 28 days) induces muscle hypertrophy and reduces muscle atrophy after sciatic nerve transection in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1139-83-9
Appearance Solid
Molecular Weight 212.20
Formula C₁₃H₈O₃
Color Off-white to light yellow
SMILES

O=C1C2=CC=CC=C2C3=CC=C(O)C=C3O1
Structure Classification
- Phenols
- Monophenols
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Proliferation/Viability AssayIFWBRT-PCR

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Journal Impact Factor

CNS Neurosci Ther

Alleviation of Microglia Mediating Hippocampal Neuron Impairments and Depression-Related Behaviors by Urolithin B via the SIRT1-FOXO1 Pathway. [Abstract]

2025 Apr;31(4):e70379. PMID: 40237232

Urolithin B purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Apr;31(4):e70379. [Abstract]

Cell viability assay of the conditional medium collected from the BV2 or HT22 cells with LPS or/and Urolithin B (UB) (0, 10, 20, 40 μmol/L) treatment.

Urolithin B purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Apr;31(4):e70379. [Abstract]

BV2 cells were pretreated with Urolithin B (UB) (0, 10, 20, 40 μmol/L) for 2 h, then treated with LPS (0.5 μg/mL) for 24 h, followed by collecting the medium as conditional medium. HT22 cells were incubated with conditional medium for 24 h, and detected the ratio of live and dead cells by staining the live cells (green) with calcein AM and dead cells (red) with PI.

Urolithin B purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Apr;31(4):e70379. [Abstract]

The protein levels of Bcl‐2 and Bax of HT22 cells after incubating with Urolithin B (UB) (0, 10, 20, 40 μmol/L) for 2 h, then treated with LPS (0.5 μg/mL) for 24 h.

Urolithin B purchased from MedChemExpress. Usage Cited in: CNS Neurosci Ther. 2025 Apr;31(4):e70379. [Abstract]

Relative mRNA levels of M1 type markers iNOS and CD86 treated with Urolithin B (UB) (0, 10, 20, 40 μmol/L).
Int Immunopharmacol

The ellagitannin metabolite urolithin C attenuated cognitive impairment by inhibiting neuroinflammation via downregulation of MAPK/NF-kB signaling pathways in aging mice. [Abstract]

2024 Sep 19;142(Pt B):113151. PMID: 39303538
Biochim Biophys Acta Mol Basis Dis

Intestinal metabolite UroB alleviates cerebral ischemia/reperfusion injury by promoting competition between TRIM65 and TXNIP for binding to NLRP3 inflammasome in response to neuroinflammation. [Abstract]

2024 Apr;1870(4):167056. PMID: 38360072
J Cell Mol Med

Urolithin B suppresses osteoclastogenesis via inhibiting RANKL-induced signalling pathways and attenuating ROS activities. [Abstract]

2022 Aug;26(16):4428-4439. PMID: 35781786
Science Essence Journal

2025.
Research Square Preprint

2021 Oct.

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (942.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.7125 mL

23.5627 mL

47.1254 mL

5 mM

0.9425 mL

4.7125 mL

9.4251 mL

10 mM

0.4713 mL

2.3563 mL

4.7125 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.80 mM); Suspended solution

This protocol yields a suspended solution of ≥ 2.08 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee G, et al. Anti-inflammatory and antioxidant mechanisms of urolithin B in activated microglia. Phytomedicine. 2019 Mar 1;55:50-57. [Content Brief]

[2]. Rodriguez J, et al. Urolithin B, a newly identified regulator of skeletal muscle mass. J Cachexia Sarcopenia Muscle. 2017 Aug;8(4):583-597. [Content Brief]

[3]. Lv MY, et al. Urolithin B suppresses tumor growth in hepatocellular carcinoma through inducing the inactivation of Wnt/β-catenin signaling. J Cell Biochem. 2019 Oct;120(10):17273-17282. [Content Brief]

[4]. Rodriguez J, et al. Urolithin B, a newly identified regulator of skeletal muscle mass. J Cachexia Sarcopenia Muscle. 2017 Aug;8(4):583-597. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.7125 mL	23.5627 mL	47.1254 mL	117.8134 mL
	5 mM	0.9425 mL	4.7125 mL	9.4251 mL	23.5627 mL
	10 mM	0.4713 mL	2.3563 mL	4.7125 mL	11.7813 mL
	15 mM	0.3142 mL	1.5708 mL	3.1417 mL	7.8542 mL
	20 mM	0.2356 mL	1.1781 mL	2.3563 mL	5.8907 mL
	25 mM	0.1885 mL	0.9425 mL	1.8850 mL	4.7125 mL
	30 mM	0.1571 mL	0.7854 mL	1.5708 mL	3.9271 mL
	40 mM	0.1178 mL	0.5891 mL	1.1781 mL	2.9453 mL
	50 mM	0.0943 mL	0.4713 mL	0.9425 mL	2.3563 mL
	60 mM	0.0785 mL	0.3927 mL	0.7854 mL	1.9636 mL
	80 mM	0.0589 mL	0.2945 mL	0.5891 mL	1.4727 mL
	100 mM	0.0471 mL	0.2356 mL	0.4713 mL	1.1781 mL

Keywords

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Urolithin B

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

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Mass Spectrometry Human Endogenous Metabolite Library

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Publications Citing Use of MedChemExpress (MCE) Urolithin B

All NF-κB Isoforms

All JNK Isoforms

All ERK Isoforms

All Akt Isoforms

All AMPK Isoforms

All Endogenous Metabolite Isoforms