Cat. No.: HY-112822

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ON-013100

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

ON-013100, an antineoplastic agent, acts a mitotic inhibitor that could inhibit Cyclin D1 expression.

For research use only. We do not sell to patients.

Purity: 99.66%
CAS No.: 865783-95-5
Formula: C₁₉H₂₂O₇S
Molecular Weight:394.44

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

ON-013100 Chemical Structure

CAS No.: 865783-95-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

ON-013100, an antineoplastic agent, acts a mitotic inhibitor that could inhibit Cyclin D1 expression.

IC₅₀ & Target^[1]

Cdk4/cyclin D1

All CDK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
2008	IC₅₀	0.005 μM Compound: 6aa	Antitumor activity against human 2008 cells by trypan blue exclusion assay Antitumor activity against human 2008 cells by trypan blue exclusion assay	[PMID: 18088089]
A549	IC₅₀	0.01 μM Compound: 6aa	Antitumor activity against human A549 cells by trypan blue exclusion assay Antitumor activity against human A549 cells by trypan blue exclusion assay	[PMID: 18088089]
AGS	IC₅₀	0.007 μM Compound: 6aa	Antitumor activity against human AGS cells by trypan blue exclusion assay Antitumor activity against human AGS cells by trypan blue exclusion assay	[PMID: 18088089]
BT-20	IC₅₀	0.01 μM Compound: 6aa	Antitumor activity against human BT20 cells by trypan blue exclusion assay Antitumor activity against human BT20 cells by trypan blue exclusion assay	[PMID: 18088089]
CEM/C2	IC₅₀	0.01 μM Compound: 6aa	Antitumor activity against human multidrug-resistant CEM/C2 cells by trypan blue exclusion assay Antitumor activity against human multidrug-resistant CEM/C2 cells by trypan blue exclusion assay	[PMID: 18088089]
COLO 205	IC₅₀	0.009 μM Compound: 6aa	Antitumor activity against human COLO205 cells by trypan blue exclusion assay Antitumor activity against human COLO205 cells by trypan blue exclusion assay	[PMID: 18088089]
Daudi	IC₅₀	0.007 μM Compound: 6aa	Antitumor activity against human Daudi cells by trypan blue exclusion assay Antitumor activity against human Daudi cells by trypan blue exclusion assay	[PMID: 18088089]
DLD-1	IC₅₀	0.003 μM Compound: 6aa	Antitumor activity against human DLD1 cells by trypan blue exclusion assay Antitumor activity against human DLD1 cells by trypan blue exclusion assay	[PMID: 18088089]
DU-145	IC₅₀	0.003 μM Compound: 6aa	Antitumor activity against AR-negative human DU145 cells by trypan blue exclusion assay Antitumor activity against AR-negative human DU145 cells by trypan blue exclusion assay	[PMID: 18088089]
HCT-116	IC₅₀	0.009 μM Compound: 6aa	Antitumor activity against human HCT116 cells by trypan blue exclusion assay Antitumor activity against human HCT116 cells by trypan blue exclusion assay	[PMID: 18088089]
HCT-15	IC₅₀	0.008 μM Compound: 6aa	Antitumor activity against human HCT15 cells by trypan blue exclusion assay Antitumor activity against human HCT15 cells by trypan blue exclusion assay	[PMID: 18088089]
K562	IC₅₀	0.004 μM Compound: 6aa	Antitumor activity against human K562 cells by trypan blue exclusion assay Antitumor activity against human K562 cells by trypan blue exclusion assay	[PMID: 18088089]
MCF7	IC₅₀	0.004 μM Compound: 6aa	Antitumor activity against ER-positive human MCF7 cells by trypan blue exclusion assay Antitumor activity against ER-positive human MCF7 cells by trypan blue exclusion assay	[PMID: 18088089]
MES-SA	IC₅₀	0.006 μM Compound: 6aa	Antitumor activity against human MES-SA cells by trypan blue exclusion assay Antitumor activity against human MES-SA cells by trypan blue exclusion assay	[PMID: 18088089]
MES-SA/Dx5	IC₅₀	0.005 μM Compound: 6aa	Antitumor activity against human multidrug-resistant MES-SA/DX5 cells by trypan blue exclusion assay Antitumor activity against human multidrug-resistant MES-SA/DX5 cells by trypan blue exclusion assay	[PMID: 18088089]
MIA PaCa-2	IC₅₀	0.004 μM Compound: 6aa	Antitumor activity against human MiaPaCa2 cells by trypan blue exclusion assay Antitumor activity against human MiaPaCa2 cells by trypan blue exclusion assay	[PMID: 18088089]
MOLT-4	IC₅₀	0.005 μM Compound: 6aa	Antitumor activity against human MOLT4 cells by trypan blue exclusion assay Antitumor activity against human MOLT4 cells by trypan blue exclusion assay	[PMID: 18088089]
NAMALVA	IC₅₀	0.006 μM Compound: 6aa	Antitumor activity against human Namalwa cells by trypan blue exclusion assay Antitumor activity against human Namalwa cells by trypan blue exclusion assay	[PMID: 18088089]
NCI-H187	IC₅₀	0.007 μM Compound: 6aa	Antitumor activity against human H187 cells by trypan blue exclusion assay Antitumor activity against human H187 cells by trypan blue exclusion assay	[PMID: 18088089]
OVCAR-3	IC₅₀	0.006 μM Compound: 6aa	Antitumor activity against human OVCAR-3 cells by trypan blue exclusion assay Antitumor activity against human OVCAR-3 cells by trypan blue exclusion assay	[PMID: 18088089]
PC-3	IC₅₀	0.008 μM Compound: 6aa	Antitumor activity against AR-positive human PC3 cells by trypan blue exclusion assay Antitumor activity against AR-positive human PC3 cells by trypan blue exclusion assay	[PMID: 18088089]
Raji	IC₅₀	0.004 μM Compound: 6aa	Antitumor activity against human Raji cells by trypan blue exclusion assay Antitumor activity against human Raji cells by trypan blue exclusion assay	[PMID: 18088089]
SK-MEL-28	IC₅₀	0.007 μM Compound: 6aa	Antitumor activity against human SK-MEL-28 cells by trypan blue exclusion assay Antitumor activity against human SK-MEL-28 cells by trypan blue exclusion assay	[PMID: 18088089]
SK-OV-3	IC₅₀	0.006 μM Compound: 6aa	Antitumor activity against human SKOV3 cells by trypan blue exclusion assay Antitumor activity against human SKOV3 cells by trypan blue exclusion assay	[PMID: 18088089]
SW480	IC₅₀	0.007 μM Compound: 6aa	Antitumor activity against human SW480 cells by trypan blue exclusion assay Antitumor activity against human SW480 cells by trypan blue exclusion assay	[PMID: 18088089]
T47D	IC₅₀	0.006 μM Compound: 6aa	Antitumor activity against ER-positive human T47D cells by trypan blue exclusion assay Antitumor activity against ER-positive human T47D cells by trypan blue exclusion assay	[PMID: 18088089]
U-87MG ATCC	IC₅₀	0.007 μM Compound: 6aa	Antitumor activity against human U87 cells by trypan blue exclusion assay Antitumor activity against human U87 cells by trypan blue exclusion assay	[PMID: 18088089]

In Vitro

ON-013100 induces apoptosis, and has activity against most human cancer cell lines in vitro. ON013100 significantly alters the cell cycle profile, dramatically increasing the number of cells in G2/M phase. Treatment of the cells with even 0.1 μM of ON013100 results in an arrest of the G2/M phase of the cell cycle^[2]. ON 013100 inhibits the proliferation of MCL (JEKO-1 and MINO), breast (MCF7 and MDA-MB-231), gastric (AGS), and esophageal (OE19, OE33, and FLO-1) cancer cell lines at a nanomolar concentration (ON 013100 GI₅₀=6.7-11.2 nM) ^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 865783-95-5
Appearance Solid
Molecular Weight 394.44
Formula C₁₉H₂₂O₇S
Color White to off-white
SMILES

OC1=CC(CS(=O)(/C=C/C2=C(OC)C=C(OC)C=C2OC)=O)=CC=C1OC
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (2)

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Journal Impact Factor

JHEP Rep

MZF1 promotes tumour progression and resistance to anti-PD-L1 antibody treatment in hepatocellular carcinoma. [Abstract]

2023 Oct 25;6(1):100939. PMID: 38074509
Exp Cell Res

NLRP6 suppresses gastric cancer growth via GRP78 ubiquitination. [Abstract]

2020 Oct 1;395(1):112177. PMID: 32682010

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (63.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5352 mL

12.6762 mL

25.3524 mL

5 mM

0.5070 mL

2.5352 mL

5.0705 mL

10 mM

0.2535 mL

1.2676 mL

2.5352 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 1.25 mg/mL (3.17 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 1.25 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 1.25 mg/mL (3.17 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cho SY, et al. Determination of the glucuronide metabolite of ON 013100, a benzylstyrylsulfone antineoplastic drug, in colon cancer cells using LC/MS/MS. J Pharm Biomed Anal. 2013 Mar 5;75:138-44. [Content Brief]

[2]. Park IW, et al. Evaluation of novel cell cycle inhibitors in mantle cell lymphoma. Oncogene. 2007 Aug 16;26(38):5635-42. [Content Brief]

[3]. Neel Jasani, et al. Potent anticancer activity of an orally bioavailable small molecule, ON 013100, and its water soluble derivative, briciclib, a clinical-stage eIF4E-targeted agent. AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5352 mL	12.6762 mL	25.3524 mL	63.3810 mL
	5 mM	0.5070 mL	2.5352 mL	5.0705 mL	12.6762 mL
	10 mM	0.2535 mL	1.2676 mL	2.5352 mL	6.3381 mL
	15 mM	0.1690 mL	0.8451 mL	1.6902 mL	4.2254 mL
	20 mM	0.1268 mL	0.6338 mL	1.2676 mL	3.1690 mL
	25 mM	0.1014 mL	0.5070 mL	1.0141 mL	2.5352 mL
	30 mM	0.0845 mL	0.4225 mL	0.8451 mL	2.1127 mL
	40 mM	0.0634 mL	0.3169 mL	0.6338 mL	1.5845 mL
	50 mM	0.0507 mL	0.2535 mL	0.5070 mL	1.2676 mL
	60 mM	0.0423 mL	0.2113 mL	0.4225 mL	1.0563 mL

Keywords

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ON-013100

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Cysteine Targeted Covalent Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

High-Efficiency Gene Editing Compound Library

Publications Citing Use of MedChemExpress (MCE) ON-013100

All CDK Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ON-013100

Cell Cycle/DNA Damage Compound Library

Kinase Inhibitor Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Cysteine Targeted Covalent Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

High-Efficiency Gene Editing Compound Library

Publications Citing Use of MedChemExpress (MCE) ON-013100

All CDK Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ON-013100 Related Classifications

Cat. No.: HY-112822