Cat. No.: HY-N2518

Agnuside

(Synonyms: Agnoside)

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Agnuside is used in the study of asthma, inflammation, and angiogenic diseases. Agnuside is an orally active compound that can be extracted from Vitex negundo.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 11027-63-7
Formula: C₂₂H₂₆O₁₁
Molecular Weight:466.44

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Agnuside Chemical Structure

CAS No.: 11027-63-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Agnuside is used in the study of asthma, inflammation, and angiogenic diseases. Agnuside is an orally active compound that can be extracted from Vitex negundo^[1]^[2]^[3]^[4].

IC₅₀ & Target

All NO Synthase Isoforms :

All COX Isoforms :

All Caspase Isoforms :

All HIF/HIF Prolyl-Hydroxylase Isoforms :

All ASCT Isoforms :

ASCT2

Cellular Effect

Cell Line	Type	Value	Description	References
HUVEC	EC₅₀	1.376 μg/mL Compound: Agnuside	Pro-angiogenic activity in HUVEC assessed as increase in cell proliferation after 96 hrs by DAPI staining-based spectrofluorometric analysis Pro-angiogenic activity in HUVEC assessed as increase in cell proliferation after 96 hrs by DAPI staining-based spectrofluorometric analysis	[PMID: 30340142]

In Vitro

Agnuside (100 μM, 12-20 h) decreases the expression of iNOS, COX-2 and IL-8 proteins, and has anti-inflammatory effect in RAW264.7 and HT-29 cells stimulated by LPS (1 μg/mL/100 ng/mL) ^[2].
Agnuside (0.1-2500 ng/mL, 20-96 h) promotes angiogenesis in HUVEC by promoting cell proliferation (EC₅₀= 1.376 µg/mL) in a time- and dose-dependent manner^[3].
Agnuside (3 μM, 4 h) significantly reduces the levels of caspase-1, ASC, NLRP3, HIF-1α, IL-1β and IL-18 to inhibit inflammation in LPS (10 μg/ml) -stimulated fibroblast-like synoviocytes (FLSs)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Agnuside (6.25 mg/kg; Oral administration; Single dose) reduces the levels of allergic inflammatory mediators in a dose-dependent manner and can inhibit allergic inflammation in Balb/C mice^[1]. Agnuside (6.25 mg/kg; Oral administration; Single dose) can inhibit autophagy in allergic asthma in Balb/C mice ^[1]. Agnuside (6.25 mg/kg; Oral administration; single dose) can reduce synovitis and fibrosis in knee osteoarthritis (KOA) in MIA-induced KOA mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Balb/C female mice model ^[1]
Dosage:

30 mg/kg, 60 mg/kg
Administration:

Oral gavage (p.o.); Single dose;
Result:

Decreased the expression of LC3B and increased the expression of Beclin1/p62 (LC3B and Beclin1/p62 are autophagy markers).
Decreased the levels of IgE and IL-4/ IL-10 in a dose-dependent manner.( IgE and IL-4/ IL-10 are allergic inflammatory mediators)

Animal Model:

KAO rat model^[4]
Dosage:

6.25 mg Monosodium iodoacetate (MIA): 1 mg
Administration:

Oral gavage (p.o.); Single dose
Result:

Alleviated the degree of local hypoxia in the synovial tissue of rats and significantly reduced the level of pro-fibrotic substances in the synovial tissue.
Inhibited the accumulation of HIF-1α and activation of NLRP3 inflammasome.

Chemical Information

CAS No. 11027-63-7
Appearance Solid
Molecular Weight 466.44
Formula C₂₂H₂₆O₁₁
Color White to yellow
SMILES

OC[C@H]([C@@H](O)[C@H](O)[C@H]1O)O[C@@]1([H])O[C@H]2[C@@]3([H])[C@](C=CO2)([H])[C@H](O)C=C3COC(C4=CC=C(O)C=C4)=O
Synonyms

Agnoside
Structure Classification
- Terpenoids
- Iridoids
- Phenols
- Monophenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Journal Impact Factor

Autophagy

ESRRA-ATG5-Mediated mitophagy enhances arginine metabolism to alleviate diabetic kidney disease. [Abstract]

2025 Dec 23:1-25. PMID: 41376268

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (214.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1439 mL

10.7195 mL

21.4390 mL

5 mM

0.4288 mL

2.1439 mL

4.2878 mL

10 mM

0.2144 mL

1.0719 mL

2.1439 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tirpude NV, et al. Agnuside mitigates OVA-LPS induced perturbed lung homeostasis via modulating inflammatory, autophagy, apoptosis-fibrosis response and myeloid lineages in mice model of allergic asthma. Int Immunopharmacol. 2022 May;106:108579. [Content Brief]

[2]. Le DD, et al. Iridoid derivatives from Vitex rotundifolia L. f. with their anti-inflammatory activity. Phytochemistry. 2023 Jun;210:113649. [Content Brief]

[3]. Pillarisetti P, Myers KA. Identification and characterization of agnuside, a natural proangiogenic small molecule. Eur J Med Chem. 2018 Dec 5;160:193-206. [Content Brief]

[4]. Zhang L, et al. Agnuside Alleviates Synovitis and Fibrosis in Knee Osteoarthritis through the Inhibition of HIF-1α and NLRP3 Inflammasome. Mediators Inflamm. 2021 Mar 16;2021:5534614. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1439 mL	10.7195 mL	21.4390 mL	53.5975 mL
	5 mM	0.4288 mL	2.1439 mL	4.2878 mL	10.7195 mL
	10 mM	0.2144 mL	1.0719 mL	2.1439 mL	5.3597 mL
	15 mM	0.1429 mL	0.7146 mL	1.4293 mL	3.5732 mL
	20 mM	0.1072 mL	0.5360 mL	1.0719 mL	2.6799 mL
	25 mM	0.0858 mL	0.4288 mL	0.8576 mL	2.1439 mL
	30 mM	0.0715 mL	0.3573 mL	0.7146 mL	1.7866 mL
	40 mM	0.0536 mL	0.2680 mL	0.5360 mL	1.3399 mL
	50 mM	0.0429 mL	0.2144 mL	0.4288 mL	1.0719 mL
	60 mM	0.0357 mL	0.1787 mL	0.3573 mL	0.8933 mL
	80 mM	0.0268 mL	0.1340 mL	0.2680 mL	0.6700 mL
	100 mM	0.0214 mL	0.1072 mL	0.2144 mL	0.5360 mL

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Agnuside

Apoptosis Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

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Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

RNA Binding Bioactive Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Agnuside

All NO Synthase Isoforms

All COX Isoforms

All Caspase Isoforms

All HIF/HIF Prolyl-Hydroxylase Isoforms

All ASCT Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Agnuside Related Classifications

HY-N2518 Related Classifications