Negundoside

Negundoside (5-400 mg/L; 1-24 h) shows no toxicity to HuH-7 cells and antagonizes CCl₄-induced cell death, with a PC₅₀ of 24.46 mg/L^[2].
Negundoside (30-100 mg/L; 25 h) protects HuH-7 cells from CCl₄-induced morphological damage to cells and nuclei, including apoptosis-like changes^[2].
Negundoside (5-100 mg/L; 1 h) dose-dependently stabilizes human erythrocyte membranes against Triton X-100-induced hemolysis, with a protective rate of 91% at 100 mg/L^[2].
Negundoside (5-100 mg/L; 20 min-24 h) dose-dependently inhibits lipid peroxidation (LPO) in rat liver microsomes treated with FeSO₄/H₂O₂ and HuH-7 cells treated with CCl₄, with maximum inhibition rates of 69% and 131% at the concentration of 100 mg/L, respectively^[2].
Negundoside (10-100 mg/L; 25 h) dose-dependently inhibits CCl₄-induced ROS and H₂O₂ production in HuH-7 cells, with a maximum inhibition rate of 104% for ROS and 143% for H₂O₂ at 100 mg/L^[2].
Negundoside (10-100 mg/L; 25 h) dose-dependently reduces CCl₄-induced intracellular Ca²⁺ elevation in HuH-7 cells, with an inhibition rate of up to 106% at 100 mg/L^[2].
Negundoside (10-100 mg/L; 25 h) inhibits the activation of caspase-3 in HuH-7 cells in a dose-dependent manner, with an inhibition rate of 139% at the concentration of 100 mg/L^[2].
Negundoside (50-100 mg/L; 40 min) inhibits cytochrome C release in isolated rat liver mitochondria, with an inhibition rate of up to 105% at 100 mg/L^[2].
Negundoside (50-100 mg/L; 25 h) protects HuH-7 cells against CCl₄-induced loss of mitochondrial membrane potential, reducing the proportion of damaged mitochondria to 20% and 11%, respectively^[2].
Negundoside (25-100 mg/L; 25 h) inhibits CCl₄-induced apoptosis in HuH-7 cells, as evidenced by reduced DNA fragmentation and a decreased proportion of cells in the sub-G₀/G₁ phase (down to 11% at 100 mg/L)^[2].
Negundoside (10-100 mg/L; 25 h) restores the reduced cAMP level in HuH-7 cells treated with CCl₄^[2].
Negundoside (10-100 mg/L; 25 h) dose-dependently inhibits CCl₄-induced activation of cPLA₂ in HuH-7 cells, with an inhibition rate reaching 304% at 50 mg/L^[2].
Negundoside (5-100 mg/L; 25 h) dose-dependently restores the depleted intracellular GSH levels in HuH-7 cells treated with CCl₄, with a recovery rate of up to 147% at 100 mg/L^[2].
Negundoside (12.5 μg/mL) inhibits the growth of Bacillus subtilis with an MIC of 12.5 μg/mL; it produces an 18 mm inhibition zone against Staphylococcus aureus, Micrococcus pyogenes, Pseudomonas aeruginosa and Escherichia coli in vitro^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.