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Cat. No.: HY-190703

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CAIX/XII-IN-20

CAIX/XII-IN-20 is a carbonic anhydrase IX/XII inhibitor with IC₅₀ values of 25 nM and 20 nM, respectively. CAIX/XII-IN-20 shows weak inhibition against the cytosolic CA I and CA II, with IC₅₀ values of 1.27 μM and 0.95 μM, respectively. CAIX/XII-IN-20 inhibits tumor-associated membrane carbonic anhydrases involved in extracellular acidification and pH regulation. CAIX/XII-IN-20 induces mitochondrial apoptosis by upregulating BAX and p53, downregulating Bcl-2, and activating caspase. CAIX/XII-IN-20 can be used for research on colorectal cancer, breast cancer, and kidney cancer.

For research use only. We do not sell to patients.

Formula: C₂₁H₂₁ClN₄O₄S₂
Molecular Weight:493.00

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (281 KB)

CAIX/XII-IN-20 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]

CA IX

25 nM (IC₅₀)

CA XII

20 nM (IC₅₀)

CA I

1.27 μM (IC₅₀)

CA Ⅱ

0.95 μM (IC₅₀)

Bax

Bcl-2

p53

Caspase

All Carbonic Anhydrase Isoforms :

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	IC50	0.575 μM	Antiproliferative activity against human 786-O renal carcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay. Antiproliferative activity against human 786-O renal carcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay.	42896302
HT-29	IC50	0.52 μM	Antiproliferative activity against human HT-29 colorectal adenocarcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay. Antiproliferative activity against human HT-29 colorectal adenocarcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay.	42896302
MCF7	IC50	0.87 μM	Antiproliferative activity against human MCF-7 breast adenocarcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay. Antiproliferative activity against human MCF-7 breast adenocarcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay.	42896302
WI-38	IC50	23.71 μM	Cytotoxicity against normal human WI-38 lung fibroblast cells assessed as viability reduction incubated 48 hrs by MTT assay. Cytotoxicity against normal human WI-38 lung fibroblast cells assessed as viability reduction incubated 48 hrs by MTT assay.	42896302

In Vitro

Anti-cancerApoptosisOthers

Anti-cancer

CAIX/XII-IN-20 (compound 7b) (48 h) shows antiproliferative activity against HT-29 (IC₅₀ = 0.52 μM), 786-O (IC₅₀ = 0.575 μM), and MCF-7 (IC₅₀ = 0.87 μM) cells, while exhibiting lower cytotoxicity toward WI-38 fibroblasts (IC₅₀ = 23.71 μM)^[1].

Anti-cancer

Apoptosis

CAIX/XII-IN-20 upregulates BAX and p53 and downregulates Bcl-2 in HT-29 cells^[1].
CAIX/XII-IN-20 activates caspase-3 and caspase-9 in HT-29 cells^[1].

Others

CAIX/XII-IN-20 potently inhibits CA IX (IC₅₀ = 0.025 μM) and CA XII (IC₅₀ = 0.020 μM), with weaker activity against CA I and CA II^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

Molecular Weight 493.00
Formula C₂₁H₂₁ClN₄O₄S₂
SMILES

NS(C(C=C1)=CC=C1NC(CSC2=NC(C3=CC=C(Cl)C=C3)C(C(C)=O)=C(C)N2)=O)(=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Saleem BAA, et al. Novel sulfonamide-containing dihydropyrimidine derivatives as potent inhibitors of tumor-associated carbonic anhydrase isoforms IX/XII: Design, synthesis, and anticancer evaluation. Bioorganic chemistry. 2026 Oct 10;184:110619. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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CAIX/XII-IN-20

All Carbonic Anhydrase Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms