2. Apoptosis Cell Cycle/DNA Damage Epigenetics MAPK/ERK Pathway Stem Cell/Wnt
  3. Apoptosis Caspase CDK PARP Bcl-2 Family ERK
  4. Calactin

Calactin is a glycoside that can be isolated from Asclepias curassavica L.. Calactin activates caspase-3, caspase-8, caspase-9, and phosphorylates ERK. Calactin induces DNA damage, apoptosis, PARP cleavage, G2/M phase cell cycle arrest, shifts Bax/Bcl-2 expression, and shows anti-proliferation effects in leukemia cells. Calactin can be used for the research of leukemia.

For research use only. We do not sell to patients.

Calactin

Calactin Chemical Structure

CAS No. : 20304-47-6

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Calactin Related Antibodies

Top Publications Citing Use of Products

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All CDK Isoform Specific Products:

View All Isoforms
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

View All PARP Isoform Specific Products:

View All Isoforms
PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

View All ERK Isoform Specific Products:

View All Isoforms
ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Calactin is a glycoside that can be isolated from Asclepias curassavica L.. Calactin activates caspase-3, caspase-8, caspase-9, and phosphorylates ERK. Calactin induces DNA damage, apoptosis, PARP cleavage, G2/M phase cell cycle arrest, shifts Bax/Bcl-2 expression, and shows anti-proliferation effects in leukemia cells. Calactin can be used for the research of leukemia[1].

IC50 & Target[1]

Caspase-3

 

Caspase-8

 

Caspase-9

 

CDK1/cyclinB1

 

Bcl-2

 

Bax

 

In Vitro

Calactin (0.065-1 μM; 0-24 h) exhibits dose-dependent and time-dependent cytotoxicity against human leukemia K562 and Molt-4 cells[1].
Calactin (0.15 μM; 0-24 h) induces G2/M phase cell cycle arrest in human leukemia K562 and Molt-4 cells by reducing the expression of Cyclin B1, Cdk1, and Cdc25C[1].
Calactin (0.15 μM; 0-24 h) induces DNA damage in human leukemia K562 and Molt-4 cells[1].
Calactin (0.15 μM; 0-24 h) induces apoptosis in human leukemia K562 and Molt-4 cells, and this apoptosis proceeds independently of caspase-3 activity[1].
Calactin (0.15 μM; 0-24 h) induces apoptosis in human leukemia K562 and Molt-4 cells through both intrinsic and extrinsic apoptotic pathways[1].
Calactin (0.15 μM; 12 h) upregulates c-Fos mRNA expression in K562 and Molt-4 cells via the ERK signaling pathway[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Calactin Related Antibodies

Cell Viability Assay[1]

Cell Line: human leukemia K562 and Molt-4 cells
Concentration: 0.065, 0.125, 0.15, 0.25, 0.5, 1 μM
Incubation Time: 0, 6, 12, 24 h
Result: Reduced cell viability in a dose-dependent and time-dependent manner.
Exhibited a LD50 of 0.161 μM for K562 cells after 12 h treatment.
Exhibited a LD50 of 0.142 μM for Molt-4 cells after 12 h treatment.
Caused progressively decreased cell viability relative to untreated controls when used at 0.15 μM for 6, 12, 18, and 24 h.

Cell Cycle Analysis[1]

Cell Line: human leukemia K562 and Molt-4 cells
Concentration: 0.15 μM
Incubation Time: 0, 6, 12, 24 h
Result: Induced a significant increase in the percentage of K562 cells arrested at the G2/M phase, from 12.8% at 0 h to 34.4% at 24 h.
Reduced the expression levels of Cyclin B1, Cdk1, and Cdc25C in both cell lines over the 24 h treatment period.

Western Blot Analysis[1]

Cell Line: human leukemia K562 and Molt-4 cells
Concentration: 0.15 μM
Incubation Time: 0, 6, 12, 24 h
Result: Increased phosphorylation levels of Chk2 and H2AX in a time-dependent manner.
Showed no change in total Chk2 or H2AX protein expression.

Apoptosis Analysis[1]

Cell Line: human leukemia K562 and Molt-4 cells
Concentration: 0.15 μM
Incubation Time: 0, 6, 12, 24 h
Result: Caused a significant increase in apoptotic cells after treatment.
Did not block calactin-induced phosphatidylserine exposure when cells were pretreated with Z-DEVD-FMK, indicating no reduction in apoptotic cell levels.

Western Blot Analysis[1]

Cell Line: human leukemia K562 and Molt-4 cells
Concentration: 0.15 μM
Incubation Time: 0, 6, 12, 24 h
Result: Induced PARP cleavage in both cell lines over the 24 h treatment period.
Increased levels of active caspase-3, caspase-8, caspase-9, and Bax in both cell lines over the 24 h treatment period.
Decreased levels of procaspase-3, procaspase-8, procaspase-9, and Bcl-2 in both cell lines over the 24 h treatment period.
Increased phosphorylation levels of ERK and Elk-1 in a time-dependent manner, with no change in total ERK or Elk-1 protein expression.
Molecular Weight

532.63

Formula

C29H40O9
CAS No.
SMILES

C(=O)[C@@]12[C@@]3([C@]([C@]4(O)[C@](C)(CC3)[C@H](CC4)C5=CC(=O)OC5)(CC[C@]1(C[C@@]6([C@@](C2)(O[C@]7(O)[C@](O6)(O[C@H](C)C[C@H]7O)[H])[H])[H])[H])[H])[H]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Calactin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calactin20304-47-6ApoptosisCaspaseCDKPARPBcl-2 FamilyERKCyclin dependent kinasepoly ADP ribose polymerase Extracellular signal regulated kinasescaspase-3Chk2caspase-8K562 cellsleukemia cellscaspase-9H2AXDNA damage response signalingapoptotic signalingInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Calactin
Cat. No.:
HY-167237
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.